Measurements of Unsaturated Vitamin B<sub>12</sub>-Binding Capacity and Myeloperoxidase as Indices of Severity of Acute Inflammation in Serial Colonoscopy Biopsy Specimens from Patients with Inflammatory Bowel Disease

Chadwick, V. S.; Schlup, Martin; Ferry, Dianne M.; Chang, A.; Butt, T. J.

doi:10.3109/00365529008998554

Cited by 17 publications

(7 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Forty‐two intestinal biopsy samples from 11 patients with CD, 59 from 18 patients with UC, and 121 from 37 non‐IBD patients were studied (Table 1). The diagnosis of CD and UC was made on the basis of established clinical, radiological, endoscopic and pathological criteria 5,21 . None of the patients were on antibiotic treatment at the time of colonoscopy.…”

Section: Methodsmentioning

confidence: 99%

Detection of Chlamydia pneumoniae by polymerase chain reaction–enzyme immunoassay in intestinal mucosal biopsies from patients with inflammatory bowel disease and controls

Chen

Liu

Wilson

et al. 2002

J of Gastro and Hepatol

View full text Add to dashboard Cite

Background and Aim : It has been suggested that Chlamydia is an organism that may have the potential to cause inflammatory bowel disease (IBD) in susceptible individuals. Chlamydia pneumoniae has emerged as an important human pathogen in the last decade. The objective of the present study was to investigate the frequency of the presence of C. pneumoniae DNA in intestinal biopsies from patients with IBD and from non-IBD controls. Methods : The DNA was extracted from 222 colonoscopic biopsies, which were obtained from 11 patients with Crohn's disease (CD), 18 patients with ulcerative colitis (UC) and from 37 non-IBD control patients. The presence of the C. pneumoniae omp1 gene and C. trachomatis 16S rRNA gene was determined using a rapid and sensitive polymerase chain reaction-enzyme immunoassay (PCR-EIA).Results : The C. pneumoniae -specific DNA was detected in 32 (14.4%) of 222 endoscopic biopsies. Among them, C. pneumoniae DNA were found in nine of 42 (21.4%) biopsies from patients with CD, nine of 59 (15.3%) biopsies from patients with UC, and 14 out of 122 (11.4%) biopsies from non-IBD control patients, respectively. Moreover, the percentage of patients with at least one biopsy positive for C. pneumoniae was higher, although not statistically significant, in CD (36.4%) and UC patients (38.9%) compared to non-IBD controls (16.2%). In contrast, C. trachomatis DNA was detected in only two of 222 (0.9%) biopsy samples. Conclusion : The C. pneumoniae DNA was detected in the intestine of both patients with IBD and in non-IBD control patients, probably reflecting the high prevalence of this organism in the environment. The moderate yield of positive biopsies in our IBD patients and the fact that the detection rate of C. pneumoniae DNA was similar in endoscopic biopsies from IBD patients and non-IBD controls does not support a direct role for this organism in the pathogenesis of IBD.

show abstract

Section: Methodsmentioning

confidence: 99%

Detection of Chlamydia pneumoniae by polymerase chain reaction–enzyme immunoassay in intestinal mucosal biopsies from patients with inflammatory bowel disease and controls

Chen

Liu

Wilson

et al. 2002

J of Gastro and Hepatol

View full text Add to dashboard Cite

show abstract

“…It is found in the secondary granules of mature neutrophils. Its role in psoriasis has not been emphasized, although levels of TCN1 protein are correlated with the severity of intestinal inflammation in Crohn's disease and RA (51)(52)(53). Elevation of TCN1 has been thought to be responsible for the elevation of vitamin B12 in a number of chronic myeloproliferative disorders (54).…”

Section: Transcobalamin 1 Up-regulationmentioning

confidence: 99%

Insights into psoriasis and other inflammatory diseases from large-scale gene expression studies

Bowcock

Shannon

et al. 2001

Human Molecular Genetics

241

199

View full text Add to dashboard Cite

Approximately 2% of the Caucasian population is affected by psoriasis (PS); a chronic inflammatory skin disease triggered by both genetic and environmental risk factors. In addition to a major contribution from the HLA class I region, PS susceptibility loci have been mapped to a number of regions including 1q21, 3q21, 4qter, 14q31-q32, 17q24-q25, 19p13.3 and 20p. Some of these overlap with loci implicated in other autoimmune/inflammatory diseases. Global gene expression studies are beginning to provide insights into the etiology of these and other complex diseases. We used Affymetrix oligonucleotide arrays comprising approximately 12 000 known genes to initiate a more comprehensive analysis of the transcriptional changes that occur in involved and uninvolved skin of 15 psoriatic patients versus six normal controls. Expression levels of the transcripts detected on the arrays were first used to determine the relationship of samples to each other using hierarchical clustering. This analysis clearly differentiated involved psoriatic skin from uninvolved and normal skin. Clusters of differentially expressed genes with similar expression patterns in the same samples were then identified. Six out of 32 clusters contained a total of 177 transcripts that were differentially expressed in involved psoriatic skin versus normal skin. These differences were independent of the gender, age, skin site and HLA class I status of the patient. Ten of the 177 genes were also differentially expressed in uninvolved skin, and several mapped to regions previously shown to harbor psoriasis susceptibility loci.

show abstract

“…MPO is an important peroxidase that exists mainly in neutrophils: its level reflects the degree of neutrophil infiltration and acts as an indirect measure of the presence of tissue inflammation [15]. Several studies demonstrated that MPO activity is related to the severity of intestinal inflammation [16,17]. Our measurements of MPO activity indicate that infiltration of inflammatory cells, especially neutrophil granulocytes, did indeed occur during the period of acute colitis induced by TNBS, since MPO levels were significantly increased in the TNBS-induced colitis group.…”

mentioning

confidence: 99%

Curcumin Improves TNBS-Induced Colitis in Rats by Inhibiting IL-27 Expression via the TLR4/NF-κB Signaling Pathway

et al. 2012

View full text Add to dashboard Cite

Curcumin is a widely used spice with anti-inflammatory and anticancer properties. It has been reported to have beneficial effects in experimental colitis. This study explored whether curcumin improves colonic inflammation in a rat colitis model through inhibition of the TLR4/NF-κB signaling pathway and IL-27 expression. After induction of colitis with 2,4,6-trinitrobenzene sulfonic acid, rats were intragastrically administered with curcumin or sulfasalazine daily for one week. Rat intestinal mucosa was collected for evaluation of the disease activity index, colonic mucosa damage index, and histological score. Myeloperoxidase activity was detected by immunohistochemistry, and mRNA and protein expression levels of TLR4, NF-κB, and IL-27 in colonic mucosa were detected by RT-PCR and Western blot. Compared with the untreated colitis group, the curcumin-treated group showed significant decreases in the disease activity index, colonic mucosa damage index, histological score, myeloperoxidase activity, and expressions of NF-κB mRNA, IL-27 mRNA, TLR4 protein, NF-κB p65 protein, and IL-27 p28 protein (p < 0.05). TLR4 mRNA expression did not differ between groups. Disease activity index decreased more rapidly in the curcumin-treated group than in the sulfasalazine-treated group (p < 0.05). There was no significant difference in TLR4, NF-κB, and IL-27 mRNA and proteins between curcumin-treated and sulfasalazine-treated groups. Curcumin shows significant therapeutic effects on 2,4,6-trinitrobenzene sulfonic acid-induced colitis that are comparable to sulfasalazine. The anti-inflammatory actions of curcumin on colitis may involve inhibition of the TLR4/NF-κB signaling pathway and of IL-27 expression.

show abstract

Measurements of Unsaturated Vitamin B₁₂-Binding Capacity and Myeloperoxidase as Indices of Severity of Acute Inflammation in Serial Colonoscopy Biopsy Specimens from Patients with Inflammatory Bowel Disease

Cited by 17 publications

References 7 publications

Detection of Chlamydia pneumoniae by polymerase chain reaction–enzyme immunoassay in intestinal mucosal biopsies from patients with inflammatory bowel disease and controls

Detection of Chlamydia pneumoniae by polymerase chain reaction–enzyme immunoassay in intestinal mucosal biopsies from patients with inflammatory bowel disease and controls

Insights into psoriasis and other inflammatory diseases from large-scale gene expression studies

Curcumin Improves TNBS-Induced Colitis in Rats by Inhibiting IL-27 Expression via the TLR4/NF-κB Signaling Pathway

Contact Info

Product

Resources

About

Measurements of Unsaturated Vitamin B12-Binding Capacity and Myeloperoxidase as Indices of Severity of Acute Inflammation in Serial Colonoscopy Biopsy Specimens from Patients with Inflammatory Bowel Disease

Cited by 17 publications

References 7 publications

Detection of Chlamydia pneumoniae by polymerase chain reaction–enzyme immunoassay in intestinal mucosal biopsies from patients with inflammatory bowel disease and controls

Detection of Chlamydia pneumoniae by polymerase chain reaction–enzyme immunoassay in intestinal mucosal biopsies from patients with inflammatory bowel disease and controls

Insights into psoriasis and other inflammatory diseases from large-scale gene expression studies

Curcumin Improves TNBS-Induced Colitis in Rats by Inhibiting IL-27 Expression via the TLR4/NF-κB Signaling Pathway

Contact Info

Product

Resources

About

Measurements of Unsaturated Vitamin B₁₂-Binding Capacity and Myeloperoxidase as Indices of Severity of Acute Inflammation in Serial Colonoscopy Biopsy Specimens from Patients with Inflammatory Bowel Disease