A microwave microstrip sensor incorporating a split ring resonator is presented in this paper for microsphere detection and dielectric characterization within a microfluidic channel. Split ring resonator (SRR) sensor of three different radii, but with approximately equal gap dimensions to change their sensitivity, were designed and fabricated, of resonance frequencies 2.5, 5.0 and 7.5 GHz. To validate the SRR sensors, two sizes of polystyrene microspheres were tested, of diameters 15 and 25 . Measurements of changes in resonance frequency and insertion loss of the odd SRR mode were related to the dielectric contrast provided by the microspheres and their host solvent, here water. COMSOL Multiphysics was used to model the sensors, and good agreements were found between the simulated and measured results.