Mechanical zona pellucida removal of vitrified-warmed human blastocysts does not affect the clinical outcome

Кириенко, К. В.; Apryshko, V.P.; Naumova, Anna; Kharitonova, Margarita A.; Клепуков, А. А.; Bolt, Anton I.; Ermilova, I. Yu.; Миронова, А. Г.; Bozina, Yana V.; Lebedeva, Elena B.; Simonenko, E.Y.; Vajta, Gábor; Yakovenko, S.A.

doi:10.1016/j.rbmo.2019.06.003

Cited by 10 publications

(5 citation statements)

References 18 publications

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“…Recent studies [22,23] suggest a superior implantation potential of frozen-thawed blastocysts with complete zona-pellucida removal, compared to partial zona-pellucida removal. Another study [24] reported similar clinical outcomes (i.e., not signi cantly detrimental or bene cial) on comparing complete zona-pellucida removal and intact zona blastocyst transfer. Whether the mechanism improved clinical outcomes of hatching and/or hatched blastocysts, compared to early blastocysts with intact zonapellucida, is still unclear.…”

Section: Discussionmentioning

confidence: 82%

In vitro fertilization outcomes of frozen-thawed embryo transfer are better with hatched blastocysts than with hatching blastocysts

Kim¹,

Park²,

Yoon³

et al. 2022

Preprint

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Purpose The aim of this study was to elucidate the effect of hatching status on in vitro fertilization outcomes in frozen–thawed blastocyst transfer cycles. Methods Frozen–thawed embryo transfer (FET) cycles performed at a single fertility center between 2016 and 2021 were retrospectively assessed. Analyses were restricted to 6821 frozen–thawed blastocyst transfers in women aged 24–47 years. For optimal comparability, double embryo transfer cycles consisting of one hatching and one hatched blastocyst were excluded. Implantation and pregnancy rates were evaluated and compared between subgroups, based on patients’ age (≤ 38 years vs. >38 years), blastocyst grade (good vs. bad grade) and number of transferred embryos (single embryo transfer [SET] vs. double embryo transfer [DET]). Results Hatched blastocyst transfer was associated with higher implantation and clinical pregnancy rates in the SET group (15.7% and 15.6%, respectively; p < 0.05). The transfer of two hatched blastocysts had a trend of higher implantation and clinical pregnancy rates, compared to the transfer of two hatching blastocysts (19.5% and 20.4%, respectively; p < 0.05) in the DET group. In the hatched blastocyst transfer group, the clinical pregnancy and implantation rates were higher, regardless of a woman’s age and embryo quality. Conclusion The IVF treatment outcomes improved when the blastocysts were hatched during FET cycles. Hence, hatched blastocyst transfer in FET cycles shall be considered as a superior method in IVF practice.

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Section: Discussionmentioning

confidence: 82%

In vitro fertilization outcomes of frozen-thawed embryo transfer are better with hatched blastocysts than with hatching blastocysts

Kim¹,

Park²,

Yoon³

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…It remains to be determined which of the components of defragmentation procedure is of a decisive importance for improving the clinical outcomes of IVF programs: directly defragmentation or previous manipulations with the zona pellucida, ie, assissted hatching. Despite the fact that assisted hatching or complete removal of the zona pellucida in good quality blastocysts does not lead to increase of effectiveness of IVF programs [23], it can have a certain positive effect in case of embryos with low morphological assessment. Thus, further comparative prospective studies are needed to assess the role of embryo defragmentation in order to increase the ongoing pregnancy rate among patients undergoing IVF infertility treatment.…”

Section: Resultsmentioning

confidence: 99%

A successful pregnancy and live birth after defragmented low quality blastocysts transfer (review of 20 clinical cases)

Kirienko¹,

Apryshko²,

Voloshanenko³

et al. 2021

Clin Obstet Gynecol Reprod Med

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“…It would be the great advantage of this study, and our practices of vitrification/warming and LAH did not affect embryonic development. Kirienko et al 37 evaluated the quality of post‐warmed blastocysts and concluded that the mechanical removal of ZP did not affect the clinical outcomes after vitrified‐warmed blastocyst transfer. However, it seems to have the potential bias of embryo quality because the good quality rate of transferred blastocyst was approximately 40% or less.…”

Section: Discussionmentioning

confidence: 99%

Laser‐assisted hatching on clinical and neonatal outcomes in patients undergoing single vitrified Blastocyst transfer: A propensity score–matched study

Endo

Mitsuhata

Hayashi

et al. 2021

Reprod Medicine & Biology

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Purpose This study determined the effect of laser‐assisted hatching on the clinical and neonatal outcomes of single vitrified blastocyst transfer. Methods From June 2014 to March 2018, 289 matched pairs after propensity score matching were analyzed. During the blastocyst warming procedure, a small section of the zona pellucida area in the empty perivitelline space was sliced off using multiple laser beams. The clinical and neonatal outcomes of the laser‐treated group and non‐treatment control were analyzed. Results In the laser‐assisted hatching group, significantly higher rates of clinical pregnancy (40.8% vs 29.4%, P < .01) and live delivery (34.3% vs 22.5%, P < .01) were observed compared to the control group. Other variables such as the average gestational weeks, the sex of the baby, birthweight, or congenital malformations were found to have no significant differences in neonatal outcomes. Moreover, all babies were singleton live births. Conclusions Single vitrified blastocyst transfer treated with laser‐assisted hatching increases the live birth rate and has no adverse effects on neonatal outcomes.

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Mechanical zona pellucida removal of vitrified-warmed human blastocysts does not affect the clinical outcome

Cited by 10 publications

References 18 publications

In vitro fertilization outcomes of frozen-thawed embryo transfer are better with hatched blastocysts than with hatching blastocysts

In vitro fertilization outcomes of frozen-thawed embryo transfer are better with hatched blastocysts than with hatching blastocysts

A successful pregnancy and live birth after defragmented low quality blastocysts transfer (review of 20 clinical cases)

Laser‐assisted hatching on clinical and neonatal outcomes in patients undergoing single vitrified Blastocyst transfer: A propensity score–matched study

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