Mechanism for the generation of superoxide anion and singlet oxygen during heme compound-catalyzed linoleic acid hydroperoxide decomposition

Koga, Satoshi; Nakano, Masahiro; Uehara, Kiyoshi

doi:10.1016/0003-9861(91)90465-u

Cited by 33 publications

(6 citation statements)

References 33 publications

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“…Dimole 1 O 2 emissions at 634 nm and 704 nm have often been used for the identification of 1 O 2 in biological systems, but unfortunately the red emission is extremely inefficient in water (10). On the other hand, most of the generated 1 O 2 could be trapped by MCLA at the rate constant of 2.9 Â 10 9 (mol/L)/s to produce an excited dioxetane analogue, which then emits light at 465 nm with high efficiency (11).…”

Section: Discussionmentioning

confidence: 99%

Rapid determination of rice seed vigour by spontaneous chemiluminescence and singlet oxygen generation during early imbibition

et al. 2002

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Using a highly sensitive single photon counter, a spontaneous chemiluminescence (CL) study on rice (Oryza sativa L.) seeds stored in different years was carried out. We first observed that the degree of ageing in rice seeds was related to the intensity of spontaneous CL during early imbibition (0-30 min). Rice seeds stored for a shorter time had a stronger intensity of CL in early imbibition. The germination rate of rice seeds showed an obvious positive correlation with the intensity of spontaneous CL. Singlet oxygen ((1)O(2)) in rice seeds during early imbibition was investigated by a CL method using a cypridina luciferin analogue, 2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo [1,2alpha] pyrazin-3-one (MCLA), as a selective CL probe. Additional experimental evidence for the formation of (1)O(2) came from the quenching effect of sodium azide (NaN(3)) on MCLA-mediated rice seeds' CL. Analysis based on the experimental results demonstrated that spontaneous CL in rice seeds during early imbibition was mainly contributed by singlet oxygen ((1)O(2)).

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Section: Discussionmentioning

confidence: 99%

Rapid determination of rice seed vigour by spontaneous chemiluminescence and singlet oxygen generation during early imbibition

et al. 2002

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“…In type I reaction, electron transfer produces free radicals. In type II [18,19]. In model system, faint emission was observed when either HpD or FCLA was omitted.…”

Section: Discussionmentioning

confidence: 93%

Localization of tumor by chemiluminescence probe during photosensitization action

Wang

Xing

et al. 2002

Cancer Letters

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“…1 O 2 concentration in the standard mixture was determined by MCLA‐dependent luminescence. 15 μM MCLA was added to the generation mixture and 1 O 2 concentration was calculated from a calibration line obtained using the HOCl+H 2 O 2 +MCLA as a standard 1 O 2 ‐mediated luminescence assay [16]. PC micelles were obtained by mixing PC in ethanol with carotenoids or α‐tocopherol (in acetone) in acetate buffer.…”

Section: Methodsmentioning

confidence: 99%

Quenching of singlet oxygen by carotenoids produced in Escherichia coli – attenuation of singlet oxygen‐mediated bacterial killing by carotenoids

et al. 2000

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We examined the viability of Escherichia coli transformants harboring various carotenoids synthesizing genes in a medium containing an enzymatic singlet oxygen generating system, which contained myeloperoxidase, hydrogen peroxide and Br 3 at pH 4.5. Singlet oxygen quenching activities of various carotenoids in phosphatidyl choline micelles in aqueous media were also studied using the same enzymatic singlet oxygen generating system. Viability of the transformants producing carotenoids was higher than that of the wild type E. coli in the singlet oxygen generation mixture. Of the transformants tested, the viability of zeaxanthin-diglucoside producing transformant was the highest. Carotenoids in increasing order of k q values were L L-carotene, a cyclic carotene 6 6 zeaxanthin with hydroxy groups 9 9 lycopene, an acyclic carotene 6 6 canthaxanthin and astaxanthin with keto groupsI Izeaxanthin-diglucoside. The k q value of zeaxanthin-diglucoside was 3.5 times higher than that of L L-carotene. These results suggest that orientation of the carotenoids in lipid layers of micelles and also in phospholipid membrane of bacteria is important for quenching of singlet oxygen. Furthermore, the viability of transformants producing lycopene and phytoene was almost as high as that of the transformant producing zeaxanthin-glucoside. ß

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Mechanism for the generation of superoxide anion and singlet oxygen during heme compound-catalyzed linoleic acid hydroperoxide decomposition

Cited by 33 publications

References 33 publications

Rapid determination of rice seed vigour by spontaneous chemiluminescence and singlet oxygen generation during early imbibition

Rapid determination of rice seed vigour by spontaneous chemiluminescence and singlet oxygen generation during early imbibition

Localization of tumor by chemiluminescence probe during photosensitization action

Quenching of singlet oxygen by carotenoids produced in Escherichia coli – attenuation of singlet oxygen‐mediated bacterial killing by carotenoids

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