Mechanism of sulfite cytotoxicity in isolated rat hepatocytes

Niknahad, Hossein; O’Brien, Peter J.

doi:10.1016/j.cbi.2008.05.032

Cited by 88 publications

(66 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A fast (within 3 h) glutathione decrease was evident in Arabidopsis wild-type and SiR-impaired leaves in response to application of sulfite (Yarmolinsky et al, 2013) and in rat hepatocytes where sulfite cytotoxicity was accompanied by a rapid disappearance of oxidized glutathione followed by a slow depletion of glutathione (Niknahad and O'Brien, 2008). Accordingly, tobacco (Nicotiana tabacum) and poplar (Populus spp.)…”

Section: Sulfite Homeostasis In Sir-impaired Tomato Plantsmentioning

confidence: 99%

Impairment in Sulfite Reductase Leads to Early Leaf Senescence in Tomato Plants

et al. 2014

View full text Add to dashboard Cite

Sulfite reductase (SiR) is an essential enzyme of the sulfate assimilation reductive pathway, which catalyzes the reduction of sulfite to sulfide. Here, we show that tomato (Solanum lycopersicum) plants with impaired SiR expression due to RNA interference (SIR Ri) developed early leaf senescence. The visual chlorophyll degradation in leaves of SIR Ri mutants was accompanied by a reduction of maximal quantum yield, as well as accumulation of hydrogen peroxide and malondialdehyde, a product of lipid peroxidation. Interestingly, messenger RNA transcripts and proteins involved in chlorophyll breakdown in the chloroplasts were found to be enhanced in the mutants, while transcripts and their plastidic proteins, functioning in photosystem II, were reduced in these mutants compared with wild-type leaves. As a consequence of SiR impairment, the levels of sulfite, sulfate, and thiosulfate were higher and glutathione levels were lower compared with the wild type. Unexpectedly, in a futile attempt to compensate for the low glutathione, the activity of adenosine-59-phosphosulfate reductase was enhanced, leading to further sulfite accumulation in SIR Ri plants. Increased sulfite oxidation to sulfate and incorporation of sulfite into sulfoquinovosyl diacylglycerols were not sufficient to maintain low basal sulfite levels, resulting in accumulative leaf damage in mutant leaves. Our results indicate that, in addition to its biosynthetic role, SiR plays an important role in prevention of premature senescence. The higher sulfite is likely the main reason for the initiation of chlorophyll degradation, while the lower glutathione as well as the higher hydrogen peroxide and malondialdehyde additionally contribute to premature senescence in mutant leaves.

show abstract

Section: Sulfite Homeostasis In Sir-impaired Tomato Plantsmentioning

confidence: 99%

Impairment in Sulfite Reductase Leads to Early Leaf Senescence in Tomato Plants

et al. 2014

View full text Add to dashboard Cite

show abstract

“…in the lung). The enzymatic oxidation of sulfite proceeds via a two-electron oxidation, but recent studies suggest that the cytotoxicity of bisulfite is mediated by free radicals (8). In fact, free radicals have been demonstrated to be produced by enzymatic initiation of the oxidation of bisulfite by prostaglandin H synthase (9) and horseradish peroxidase (10,11), with formation of the ⅐ SO 3 Ϫ anion radical as follows: where k Ͼ 2 ϫ 10 9 M Ϫ1 s Ϫ1 (13).…”

mentioning

confidence: 99%

Protein Radical Formation Resulting from Eosinophil Peroxidase-catalyzed Oxidation of Sulfite

Ranguelova

Chatterjee

Ehrenshaft

et al. 2010

Journal of Biological Chemistry

View full text Add to dashboard Cite

“…In S. meliloti 1021, the expression of the YedY-related gene SMc01281 was highly induced during growth on taurine and thiosulfate, indicating a possible role for this enzyme in sulfur metabolism (46). It has been reported that sulfite may cause cytotoxicity by increasing free radical formation and lipid peroxidation and disabling the cellular antioxidant defense system by depleting reduced glutathione (GSH) and lowering the activities of catalase and glutathione peroxidase (47)(48)(49). Therefore, we speculated that a yedYZ deletion may influence S. meliloti CCNWSX0020 sulfite metabolism and potentially further impair the antioxidant defense which copes with reactive oxygen species (ROS) generated by excess heavy metals.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Response to Heavy Metals in Sinorhizobium meliloti CCNWSX0020 Reveals New Metal Resistance Determinants That Also Promote Bioremediation by Medicago lupulina in Metal-Contaminated Soil

Jiao

Gao

et al. 2017

Appl Environ Microbiol

View full text Add to dashboard Cite

The symbiosis of the highly metal-resistant Sinorhizobium meliloti CCNWSX0020 and Medicago lupulina has been considered an efficient tool for bioremediation of heavy metal-polluted soils. However, the metal resistance mechanisms of S. meliloti CCNWSX00200 have not been elucidated in detail. Here we employed a comparative transcriptome approach to analyze the defense mechanisms of S. meliloti CCNWSX00200 against Cu or Zn exposure. Six highly upregulated transcripts involved in Cu and Zn resistance were identified through deletion mutagenesis, including genes encoding a multicopper oxidase (CueO), an outer membrane protein (Omp), sulfite oxidoreductases (YedYZ), and three hypothetical proteins (a CusA-like protein, a FixH-like protein, and an unknown protein), and the corresponding mutant strains showed various degrees of sensitivity to multiple metals. The Cu-sensitive mutant (ΔcueO) and three mutants that were both Cu and Zn sensitive (ΔyedYZ, ΔcusA-like, and ΔfixH-like) were selected for further study of the effects of these metal resistance determinants on bioremediation. The results showed that inoculation with the ΔcueO mutant severely inhibited infection establishment and nodulation of M. lupulina under Cu stress, while inoculation with the ΔyedYZ and ΔfixH-like mutants decreased just the early infection frequency and nodulation under Cu and Zn stresses. In contrast, inoculation with the ΔcusA-like mutant almost led to loss of the symbiotic capacity of M. lupulina to even grow in uncontaminated soil. Moreover, the antioxidant enzyme activity and metal accumulation in roots of M. lupulina inoculated with all mutants were lower than those with the wildtype strain. These results suggest that heavy metal resistance determinants may promote bioremediation by directly or indirectly influencing formation of the rhizobiumlegume symbiosis.IMPORTANCE Rhizobium-legume symbiosis has been promoted as an appropriate tool for bioremediation of heavy metal-contaminated soils. Considering the plantgrowth-promoting traits and survival advantage of metal-resistant rhizobia in contaminated environments, more heavy metal-resistant rhizobia and genetically manipulated strains were investigated. In view of the genetic diversity of metal resistance determinants in rhizobia, their effects on phytoremediation by the rhizobium-legume symbiosis must be different and depend on their specific assigned functions. Our work provides a better understanding of the mechanism of heavy metal resistance determinants involved in the rhizobium-legume symbiosis, and in further studies, genetically Citation Lu M, Jiao S, Gao E, Song X, Li Z, Hao X, Rensing C, Wei G. 2017. Transcriptome response to heavy metals in Sinorhizobium meliloti CCNWSX0020 reveals new metal resistance determinants that also promote bioremediation by Medicago lupulina in metalcontaminated soil. Appl Environ Microbiol

show abstract

Mechanism of sulfite cytotoxicity in isolated rat hepatocytes

Cited by 88 publications

References 30 publications

Impairment in Sulfite Reductase Leads to Early Leaf Senescence in Tomato Plants

Impairment in Sulfite Reductase Leads to Early Leaf Senescence in Tomato Plants

Protein Radical Formation Resulting from Eosinophil Peroxidase-catalyzed Oxidation of Sulfite

Transcriptome Response to Heavy Metals in Sinorhizobium meliloti CCNWSX0020 Reveals New Metal Resistance Determinants That Also Promote Bioremediation by Medicago lupulina in Metal-Contaminated Soil

Contact Info

Product

Resources

About