Mechanisms of natural brassinosteroid-induced apoptosis of prostate cancer cells

Steigerova, Jana; Rárová, Lucie; Oklešťková, Jana; Křížová, Kateřina; Levková, Monika; Šváchová, Michaela; Kolář, Zdeněk; Strnad, Miroslav

doi:10.1016/j.fct.2012.08.031

Cited by 45 publications

(46 citation statements)

References 34 publications

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“…BS treatment resulted in arrest of cell cycle in the G1 phase and an induction of apoptosis [104,110]. A range of techniques including flow cytometry, Western blotting, TUNEL, DNA ladder assays and immunofluorescence analyses was used for study of BS-induced apoptosis of human prostate cancer cell lines LNCaP and DU-145 [111]. Cell growth inhibition and G 1 cell cycle arrest were accompanied by reductions in cyclin D1, CDK4/6 and pRb expression.…”

Section: Anticancer Effectsmentioning

confidence: 99%

Steroid plant hormones: Effects outside plant kingdom

2015

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Section: Anticancer Effectsmentioning

confidence: 99%

Steroid plant hormones: Effects outside plant kingdom

2015

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“…On the other hand, a growing body of data suggests that brassinosteroids might be the putative inducers of the activity of the glucocorticoid receptor. These considerations are based on the effects of brassinosteroids in human systems Steigerová et al 2012), and the resemblance of plant steroid signalling processes to those of ecdysteroids in Drosophila (Thummel and Chory 2002), both sharing a range of common developmental and physiological responses to stress. To support this idea, there is a large body of evidence for the anti-stress behaviour of brassinosteroids (Dhaubhadel et al 2002;Choudhary et al 2012;Kanwar et al 2012;Li et al 2012).…”

Section: Discussionmentioning

confidence: 99%

“…Rárová et al (2012) showed that brassinosteroids inhibit angiogenesis in human endothelial cells and interact with human steroid receptors such as the oestrogen receptors a and b and the androgen receptor. Moreover, Steigerová et al (2012) found that brassinosteroids mediated the apoptosis of human cancer cells by inducing cellular block and stopping cells at the G1 stage. The glucocorticoid receptor (GR) is a mammalian nuclear hormone-receptor transcription factor (Giguere et al 1986) that is activated by glucocorticoids, a class of steroid hormones, synthesized in the adrenal cortex, and popular as anti-inflammatory and immunosuppressive therapeutic agents (Mangelsdorf et al 1995).…”

Section: Introductionmentioning

confidence: 99%

The rat glucocorticoid receptor integration in Nicotiana langsdorffii genome affects plant responses to abiotic stresses and to arbuscular mycorrhizal symbiosis

Bogani

Calistri

Biricolti

et al. 2015

Plant Biotechnol Rep

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The present study reports evidence of the pleiotropic effects caused by the insertion of the rat glucocorticoid receptor (GR) into the genome of Nicotiana langsdorffii. Transgenic N. langsdorffii-GR plants and the wild-type genotypes were analysed for their phenotypic and physiological characteristics. The integration of the GR gene affected flowering, growth habit, leaf morphology and stomatal pattern. Furthermore, GR plants showed an increased tolerance to heavy metal, drought and heat stress as evidenced by electrolyte leakage and by cell dedifferentiation and differentiation capability after recovery from stress treatments. We also monitored the establishment of the beneficial symbiosis between transgenic plants and the mycorrhizal fungus Funneliformis mosseae whose presymbiotic growth was significantly reduced by root exudates of N. langsdorffii-GR plants. The observed pleiotropic responses of transgenic plants may be a consequence of the hormonal imbalance, putatively due to the interaction of the GR receptor with the host genetic background. Our findings suggest that N. langsdorffii-GR plants can be used as a functional model system for the study of plant responses to a series of environmental stimuli.

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“…The mechanism of antiproliferative activity of 28-homoCS and 24-EpiBL was studied by Steigerova et al [51,65] in prostate cancer cell lines, DU-145 and LNCaP (hormone insensitive and sensitive). Various techniques like flow cytometry, TUNEL DNA ladder Test, Western blotting, and immunofluorescence were used to study the effect of these BRs on prostate cancer cells.…”

Section: Anticancerous/antiproliferative Activitiesmentioning

confidence: 99%

Therapeutic Potential of Brassinosteroids in Biomedical and Clinical Research

Kohli

Bhardwaj

et al. 2020

Biomolecules

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Steroids are a pivotal class of hormones with a key role in growth modulation and signal transduction in multicellular organisms. Synthetic steroids are widely used to cure large array of viral, fungal, bacterial, and cancerous infections. Brassinosteroids (BRs) are a natural collection of phytosterols, which have structural similarity with animal steroids. BRs are dispersed universally throughout the plant kingdom. These plant steroids are well known to modulate a plethora of physiological responses in plants leading to improvement in quality as well as yield of food crops. Moreover, they have been found to play imperative role in stress-fortification against various stresses in plants. Over a decade, BRs have conquered worldwide interest due to their diverse biological activities in animal systems. Recent studies have indicated anticancerous, antiangiogenic, antiviral, antigenotoxic, antifungal, and antibacterial bioactivities of BRs in the animal test systems. BRs inhibit replication of viruses and induce cytotoxic effects on cancerous cell lines. Keeping in view the biological activities of BRs, this review is an attempt to update the information about prospects of BRs in biomedical and clinical application.

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Mechanisms of natural brassinosteroid-induced apoptosis of prostate cancer cells

Cited by 45 publications

References 34 publications

Steroid plant hormones: Effects outside plant kingdom

Steroid plant hormones: Effects outside plant kingdom

The rat glucocorticoid receptor integration in Nicotiana langsdorffii genome affects plant responses to abiotic stresses and to arbuscular mycorrhizal symbiosis

Therapeutic Potential of Brassinosteroids in Biomedical and Clinical Research

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