Media development for large scale <i>Agrobacterium tumefaciens</i> culture

Leth, Ingrid K.; McDonald, Karen A.

doi:10.1002/btpr.2504

Cited by 8 publications

(6 citation statements)

References 33 publications

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“…The vitality of A. tumefaciens was found to be in uenced by the bacterium culture medium, growth period, and environmental conditions. In this study, we utilized YEP medium for culturing A. tumefaciens, as it is known to be particularly suitable for A. tumefaciens cultivation 47 . After an overnight recovery in the shaker, the EHA105 strain demonstrated robust vitality with rapid multiplication rates.…”

Section: Discussionmentioning

confidence: 99%

A simple and efficient Agrobacterium tumefaciens mediated transgenic system for tetraploid potato cultivar Desiree

Xu,

Qi,

Chang

et al. 2024

Preprint

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As the fourth staple food crop in the world, potatoes can provide enough energy, protein, and nutrients necessary for humans. However, the population growth and negative effects of climate change call for improved potato yields and resilience. Genetic engineering is a convenient way to improve potato varieties and create new germplasm resources. Agrobacterium tumefaciens mediated transformation is a stable and widely used method for genetic improvement. In this study, a simple and efficient transgenic system was built by testing different potato materials, explants type, selection medium, selection agent, A. tumefaciens EHA105 strain carried different vectors. The results shown this simple system produced positive transformed seedlings in about 40 days. The lower ploidy change ratio for tetraploid variety Desiree compared to diploid recipient. Both SEG and SE could function as a single regeneration medium. Leaf discs explants is eligible. The concentration of 20μg/L is suitable for chlorsulfuron selection. The positive transgenic efficiency of tetraploid recipient Desiree is up to 68.79%, This simple and fast transgenic system provides a powerful tool for supporting basic research on potato functional genes and creating new potato materials for genetic engineering breeding.

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Section: Discussionmentioning

confidence: 99%

A simple and efficient Agrobacterium tumefaciens mediated transgenic system for tetraploid potato cultivar Desiree

Xu,

Qi,

Chang

et al. 2024

Preprint

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“…Indeed, the ambiguity of the composition of LB leads to ambiguity in the spectroscopy and the interpretability of the behavior of the various contributors to the IE. This situation prevails in spite of a strong shift in research and applied bacteriology [25][26][27][28][29][30] toward use of so-called ''chemically defined'' media (CD), of which minimal medium (MM) is an example.…”

Section: Problems In Characterizing and Monitoring Bacterial Culturesmentioning

confidence: 99%

“…CD media provide improved control and reproducibility of the cultures. [25][26][27][28][29][30] LB media contain a broad range of materials, such as proteins, carbohydrates, and lipids as relatively unrefined biological sources, e.g., yeast extract or trypsinized milk. They are thus complex mixtures that defy the detailed analysis that is possible with CD media.…”

Section: Problems In Characterizing and Monitoring Bacterial Culturesmentioning

confidence: 99%

“…However, it may prove difficult to assign the source(s) of the changes. For CD media like MM on the other hand [25][26][27][28][29][30] spontaneous Raman spectroscopy provides a simple, direct approach to establish the chemical state of the medium without physical sampling. In this paper we show sufficient Raman spectra to demonstrate that such features can serve as IE for practical purposes.…”

Section: Bsn Algorithmmentioning

confidence: 99%

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Coupled Turbidity and Spectroscopy Problems: A Simple Algorithm for Volumetric Analysis of Optically Thin or Dilute, In Vitro Bacterial Cultures in Various Media

et al. 2020

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An approach binary spectronephelometry (BSN) to perform real-time simultaneous noninvasive in situ physical and chemical analysis of bacterial cultures in fluid media is described. We choose to characterize cultures of Escherichia coli (NC), Pseudomonas aeruginosa (PA), and Shewanella oneidensis (SO) in the specific case of complex media whose Raman spectrum cannot be unambiguously assigned. Nevertheless, organism number density and a measure of the chemical makeup of the fluid medium can be monitored noninvasively, simultaneously, and continuously, despite changing turbidity and medium chemistry. The method involves irradiating a culture in fluid medium in an appropriate vessel (in this case a standard 1 cm cuvette) using a near infrared laser and collecting all the backscattered light from the cuvette, i.e., the Rayleigh–Mie line and the inelastically emitted light which includes unresolved Raman scattered light and fluorescence. Complex “legacy” media contain materials of biological origin whose chemical composition cannot be fully delineated. We independently calibrate this approach to a commonly used reference, optical density at 600 nm (OD600) for characterizing the number density of organisms. We suggest that the total inelastically emitted light could be a measure of the chemical state of a biologically based medium, e.g., lysogeny broth (LB). This approach may be useful in a broad range of basic and applied studies and enterprises that utilize bacterial cultures in any medium or container that permits optical probing in the single scattering limit.

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“…33 In a study in which a chemically defined media was developed for growing Agrobacterium tumefaciens at large scale for commercial production of recombinant proteins by transient expression in plants, PBD was employed. 35 On the other hand, in recent times, researchers have been modeling the results of their own fermentation studies. The maximum specific growth rate of Agrobacterium tumefaciens in a chemically defined media was 0.33 hr −1 and the final biomass concentration was found as 2.6 g dry cell weight/L.…”

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confidence: 99%

Inulinase production and mathematical modeling from carob extract by using Aspergillus niger

Ilgın

Germeç

Turhan

2019

Biotechnology Progress

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The main objectives of the study were to produce inulinase from carob extract by Aspergillus niger A42 (ATCC 204447) and to model the inulinase fermentation in the optimum carob extract-based medium. In the study, carob extract was used as a novel and renewable carbon source in the production of A. niger inulinase. For medium optimization, eight different variables including initial sugar concentration ( Bx), (NH 4 ) 2 HPO 4 , MgSO 4 .7H 2 O, KH 2 PO 4 , NH 4 NO 3 , yeast extract, peptone, and ZnSO 4 .7H 2 O were employed. After fermentations, optimum medium composition contained 1% yeast extract in 5 Bx carob extract. As a result of the fermentation, the maximum inulinase activity, maximum invertase-type activity, I/S ratio, maximum inulinase-and invertasetype activity rates, maximum sugar consumption rate, and sugar utilization yield were 1507.03 U/ml, 1552.86 U/ml, 0.97, 175.82 and 323.76 U/ml/day, 13.26 g/L/day, and 98.52%, respectively. Regarding mathematical modeling, the actual inulinase production and sugar consumption data were successfully predicted by Baranyi and Cone models based on the model evaluation and validation results and the predicted kinetic values, respectively. Consequently, this was the first report in which carob extract was used in the production of inulinase as a carbon source. Additionally, the best-selected models can serve as universal equations in modeling the inulinase production and sugar consumption in shake flask fermentation with carob extract medium. K E Y W O R D S Aspergillus, carob extract, flexible models, fungal inulinase, optimization 1 | INTRODUCTION Developments in the field of biotechnology play an important role in the effective use of all kinds of agricultural products and waste in the world. The agricultural products which are rich in food additive and sugar but limited in evaluation potential are used in the production of high added-value products by biotechnological processes. 1,2 The enzyme industry is the result of a rapid development seen primarily over the past four decades thanks to the evolution of modern biotechnology. This development allowed the introduction of enzymes into true industrial products and processes, for example, within the detergent, textile, and starch industries. 3 Inulinase (2,1-β-D-fructanohydrolase EC 3.2.1.7) targets on the β-2,1 linkage of inulin and hydrolyzes it into fructose. Inulin and inulinase can be used for production of either ultra-high fructose syrups, with D-fructose content over 95% by exo-enzymatic hydrolysis, or for production of oligofructose syrups by endo-enzymatic hydrolysis. 4 Inulinases are classified into endo-and exoinulinases, depending on their mode of action. Endo-inulinases (2,1-β-D-fructan fructanohydrolase; EC 3.2.1.7) are specific for inulin and hydrolyze it by breaking bonds between fructose units that are located away from the ends of the polymer network, to produce oligosaccharides. Exoinulinases (β-D-fructohydrolase; EC 3.2.1.8) split terminal fructose units from the nonreducing end of the inulin mo...

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Media development for large scale Agrobacterium tumefaciens culture

Cited by 8 publications

References 33 publications

A simple and efficient Agrobacterium tumefaciens mediated transgenic system for tetraploid potato cultivar Desiree

A simple and efficient Agrobacterium tumefaciens mediated transgenic system for tetraploid potato cultivar Desiree

Coupled Turbidity and Spectroscopy Problems: A Simple Algorithm for Volumetric Analysis of Optically Thin or Dilute, In Vitro Bacterial Cultures in Various Media

Inulinase production and mathematical modeling from carob extract by using Aspergillus niger

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