Media for the Aerobic Resuscitation of Campylobacter jejuni

Abstract

The microaerophilic nature of Campylobacter jejuni has complicated its recovery from human and animal sources. In this study, enhancement of the growth and aerotolerance of C. jejuni ATCC 35921 in nutrient broth no. 2 (NB2) was investigated. The efficiency of recovery of C. jejuni in NB2 containing FBP (0.025% [each] ferrous sulfate, sodium metabisulfite, and sodium pyruvate), 5% laked horse blood, hemin, Oxyrase, or activated charcoal in an aerobic atmosphere was compared with that obtained under microaerophilic incubation. The shortest lag time (lamda) for cells grown aerobically was observed with NB2 supplemented with FBP, 5% laked horse blood, 0.01 g/liter of hemin, or 0.15 U/ml of Oxyrase. The efficacy of these media to resuscitate C. jejuni cells in late exponential phase, as well as cells subjected to stress induced by cold, heat, starvation, or acid, was determined in aerobic or microaerobic atmospheres. The h of cells grown aerobically in NB2 containing both FBP and blood was similar to that obtained in the same medium incubated in a microaerobic environment (P > 0.05). However, the X was longer during aerobic growth when low numbers of cells (approximately 1 log CFU/ml) in late exponential phase were used as the initial inoculum. The best recovery of stressed C. jejuni was observed in NB2 supplemented with FBP and blood and incubated aerobically. Enrichment in media incorporating FBP and 5% laked horse blood is a simple, convenient, and time-saving method to replace microaerophilic incubation methods for the resuscitation of C. jejuni.

Análisis molecular de la resistencia a fluoroquinolonas y macrólidos en aislados de Campylobacter jejuni de humanos, bovinos y carne de ave

“…Las cepas fueron recuperadas de cepario en agar Skirrow con el factor de crecimiento FBP 22 e incubadas en microaerofilia a 42°C por 48 h. La confirmación de las cepas de C. jejuni se realizó por las características de la colonia, características a la tinción, hidrólisis de hipurato y por la prueba de reacción de polimerasa en cadena (RPC) que amplifica el gen potencial de la oxido-reductasa 23 . Estudio de susceptibilidad in vitro: se realizó mediante la determinación de la concentración inhibitoria mínima (CIM) por el método de microdilución en caldo acorde a las recomendaciones del CLSI [16][17][18] .…”