Mediated Enzyme Electrode for the Determination of L-Glutamate

Vahjen, W.; Bradley, J.; Bilitewski, Ursula; Schmid, Rolf D.

doi:10.1080/00032719108052983

Cited by 30 publications

(13 citation statements)

References 5 publications

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“…A lot of foodstuff contains small amounts of glutamate [3, 4], which gives food “beef” taste. Therefore, glutamate is often used as a flavor enhancer.…”

Section: Introductionmentioning

confidence: 99%

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A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite

et al. 2017

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In this work, we developed a new amperometric biosensor for glutamate detection using a typical method of glutamate oxidase (GlOx) immobilization via adsorption on silicalite particles. The disc platinum electrode (d = 0.4 mm) was used as the amperometric sensor. The procedure of biosensor preparation was optimized. The main parameters of modifying amperometric transducers with a silicalite layer were determined along with the procedure of GlOx adsorption on this layer. The biosensors based on GlOx adsorbed on silicalite demonstrated high sensitivity to glutamate. The linear range of detection was from 2.5 to 450 μM, and the limit of glutamate detection was 1 μM. It was shown that the proposed biosensors were characterized by good response reproducibility during hours of continuous work and operational stability for several days. The developed biosensors could be applied for determination of glutamate in real samples.

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“…A lot of foodstuff contains small amounts of glutamate [3, 4], which gives food “beef” taste. Therefore, glutamate is often used as a flavor enhancer.…”

Section: Introductionmentioning

confidence: 99%

“…Among electrochemical biosensors, the amperometric ones are considered to be the most promising, and they are most often used to determine glutamate [1, 4, 5, 7, 12]. Besides, the potentiometric electrodes (NH 4+ detection) can be an alternative; however, they are less sensitive.…”

Section: Introductionmentioning

confidence: 99%

A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite

et al. 2017

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“…This technique requires the immobilization of glutamate oxidase or dehydrogenase on an electrode surface. Glutamate biosensors that are based on glutamate dehydrogenase require the coimmobilization of a coenzyme, NADP + or NADH, and an electron mediator (Jeffries 1997, Lobo 1997, Doaga 2009, Vahjen 1991). Stable immobilization of the coenzyme and the low stability of this enzyme (Amine 1992) are the main problems associated with the use of dehydrogenase based detection schemes.…”

Section: Electrochemical Detection Of Monoamine Neurotransmittersmentioning

confidence: 99%

Recent Developments in Electrochemical Sensors for the Detection of Neurotransmitters for Applications in Biomedicine

2015

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Neurotransmitters are important biological molecules that are essential to many neurophysiological processes including memory, cognition, and behavioral states. The development of analytical methodologies to accurately detect neurotransmitters is of great importance in neurological and biological research. Specifically designed microelectrodes or microbiosensors have demonstrated potential for rapid, real-time measurements with high spatial resolution. Such devices can facilitate study of the role and mechanism of action of neurotransmitters and can find potential uses in biomedicine. This paper reviews the current status and recent advances in the development and application of electrochemical sensors for the detection of small-molecule neurotransmitters. Measurement challenges and opportunities of electroanalytical methods to advance study and understanding of neurotransmitters in various biological models and disease conditions are discussed.

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“…Також в невеликих кількостях він міститься в багатьох продуктах харчування [2,3]. Присутність в їжі глутамату надає їй «м'ясного» смаку, тому глу-тамат часто застосовують в якості підсилюва-ча смаку, додаючи його до багатьох харчових продуктів.…”

Section: Yu Kucherenko I S Kucherenko O O Soldatkin т O unclassified