Mediators of cellular immune reactions

Hanson, Jennifer M.; Rumjanek, Vivian M.; Morley, John E.

doi:10.1016/0163-7258(82)90010-9

Cited by 13 publications

(6 citation statements)

References 232 publications

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“…Additionally, they demonstrated the applicability of the method in the study of cell adherence on protein coated surfaces, cultivating bone-marrow derived macrophages [138] and fibroblasts [139] on surfaces coated with fibronectin, gelatin and serum proteins. In the case of macrophages, the cells were activated by introducing heat-killed Listeria monocytogenes, lipopolysaccharide and interferon-g, which initiate biochemical processes, such as increased protein synthesis and Ca 2þ uptake, resulting in morphological changes [140]. These effects were immediately registered as an increase in the real component of the measured impedance (resistance).…”

Section: Impedance Measurements On Cells Adhering To a Growth Substratementioning

confidence: 99%

Chip Based Electroanalytical Systems for Cell Analysis

et al. 2008

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This review with 239 references has as its aim to give the reader an introduction to the kinds of methods used for developing microchip based electrode systems as well as to cover the existing literature on electroanalytical systems where microchips play a crucial role for nondestructive measurements of processes related to living cells, i.e., systems without lysing the cells. The focus is on chip based amperometric and impedimetric cell analysis systems where measurements utilizing solely carbon fiber microelectrodes (CFME) and other nonchip electrode formats, such as CFME for exocytosis studies and scanning electrochemical microscopy (SECM) studies of living cells have been omitted. Included is also a discussion about some future and emerging nano tools and considerations that might have an impact on the future of "nondestructive" chip based electroanalysis of living cells.

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Section: Impedance Measurements On Cells Adhering To a Growth Substratementioning

confidence: 99%

Chip Based Electroanalytical Systems for Cell Analysis

et al. 2008

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“…The migration of macro phages from animals exhibiting antibody-mediated hypersensitivity is not influenced by antigen (GEORGE and VAUGHAN 1962), nor is the presence of antigen necessary for MIF to exhibit its biological activity, so making it highly unlikely that this product of lymphocyte activation can be related to classical antibody, or to complement components. This topic has been reviewed at length elsewhere (HANSON et al 1982). histamine, kinins, prostaglandins) than to antibodies, with their dependence upon selective recognition of antigen.…”

Section: B Lymphokinesmentioning

confidence: 99%

“…The term encompasses the mediators derived from activated T -lymphocytes, i.e. Historically, the concept of special mediators of immunological regulation, rather than direct cellular or antibody-mediated mechanisms, evolved from studies of delayed-type hypersensitivity and inflammation, and has been extensively documented (ADELMAN et al 1979;ROCKLIN et al 1980;WATSON 1981;HANSON et al 1982;DUMONDE and HAMBLIN 1983). monokines (MKs) r:vv AKSMAN and NAMBA 1976); and those mediators whose cellular origin is known to be more diverse, e.g.…”

Section: A Introductionmentioning

confidence: 99%

Pharmacology of the Skin I

Greaves¹,

Shuster²

1989

Handbook of Experimental Pharmacology

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“…In searching for new therapeutically effective substances e. g. for cancer treatment, immune deficiency syndromes or problematic multifactorial infectious diseases, many possibilities have been shown to enhance non-specific defence mechanisms (13,14,16). In the course of these investigations it became evident that in the complex system of humoral and cellular defence mechanisms a central transmitter role is played by soluble, noncellular biosubstances (8,26). In principle, these are similar to hormones, are usually called mediators and they coordinate cooperation of different effector cell populations (7,22).…”

Section: Introductionmentioning

confidence: 99%

“…In principle, these are similar to hormones, are usually called mediators and they coordinate cooperation of different effector cell populations (7,22). These cells, e. g. Tand B-lymphocytes, helper-, suppressor-, NK-cells o r macrophages, are at least performing the actual defence mechanisms such as cytotoxicity, phagocytosis or antibody-production (8,18).…”

Section: Introductionmentioning

confidence: 99%

Enhancement of Non‐Specific Antiviral Defence Mechanisms in NMRI‐Mice by Cell Culture Mediators

Reubel

Büttner

Mayr

1986

Journal of Veterinary Medicine, Series B

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Summary Through in vitro stimulation of different cell types with various stimulating substances, production of mediators with antiviral activity in vivo was studied. In two lethal virus mouse challenge models — adult NMRI‐mice challenged with pseudorabies virus (PRV) and baby mice challenged with stomatitis vesicularis virus (VSV) — antiviral efficacy of cell culture supernatants (CCSs) was demonstrated. In a plaque reduction assay, interferon activity in CCSs and in serum of CCS‐treated mice was investigated. Prophylactic treatment with CCSs from murine hybridomas and human lymphomas — stimulated by inactivated parapoxvirus ovis — reduced the mortality rate of challenged mice significantly. None of the cell cultures secreted interferon into the medium, whereas intraperitoneal application of some CCSs induced interferon activity in serum of mice. A possibility for production of non‐antigen‐specific mediators was shown by appropriate stimulation of certain cell types in vitro. Prophylactic treatment with these mediators triggered non‐specific defence mechanisms in mice against two lethal virus infections. Zusammenfassung Steigerung der unspezifischen Virusabwehr bei NMRI‐Mäusen durch Mediatoren aus Zellkulturen Mit verschiedenen Stimulationssubstanzen wurde versucht, in vitro unterschiedliche Zellarten zur Sekretion von Mediatoren mit antiviraler Wirksamkeit in vivo anzuregen. Die Prüfung der antiviralen Wirksamkeit der Zellkulturüberstände (ZKÜ) erfolgte in zwei Virusbelastungsmodellen, wobei adulte NMRI‐Mäuse mit Aujeszky‐Virus und juvenile Mäuse mit Vesicularis Stomatitis‐Virus belastet wurden. Mit einem Plaque‐Reduktions‐Test wurde die Interferon (IFN)‐Aktivität in einigen ZKÜ und im Serum ZKÜ‐behandelter Tiere bestimmt. Vorbeugende Behandlung mit ZKÜ aus murinen Hybridomas bzw. humanen Lymphomas, die mit inaktiviertem Parapoxvirus ovis stimuliert waren, verringerte die Mortalitätsrate von virusbelasteten Mäusen erheblich. Keine der Zellkulturen sezernierte IFN in den Überstand, während die intraperitoneale Verabreichung einiger ZKÜ IFN‐Aktivität im Serum der Mäuse induzierte. Die Versuche zeigen die Möglichkeit auf, bestimmte Zellarten mit geeigneten Stimulationssubstanzen zur Sekretion von Mediatoren anzuregen, mit denen sich bei Mäusen in vivo die unspezifische Infektabwehr gegen bestimmte Virusinfektionen erhöhen läßt.

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Mediators of cellular immune reactions

Cited by 13 publications

References 232 publications

Chip Based Electroanalytical Systems for Cell Analysis

Chip Based Electroanalytical Systems for Cell Analysis

Pharmacology of the Skin I

Enhancement of Non‐Specific Antiviral Defence Mechanisms in NMRI‐Mice by Cell Culture Mediators

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