Medical applications of dust-free rooms. I. Elimination of airborne bacteria in a research laboratory.

Coriell, Lewis L.; McGarrity, Gerard J.; Horneff, J

doi:10.2105/ajph.57.10.1824

Cited by 21 publications

(4 citation statements)

References 10 publications

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“…The vertical air velocity used in this hood (19 cm/s) is substantially lower than that conventionally employed in laminar air flow systems (50 cm/s), but we have shown that lowered air velocities are effective in elimination of airborne contamination in tissue culture laboratories and operating rooms and elimination of airborne infection in animal laboratories in controlled studies (5,7,11,12).…”

Section: Resultsmentioning

confidence: 88%

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Mass Airflow Cabinet for Control of Airborne Infection of Laboratory Rodents

McGarrity¹,

Coriell²

1973

Applied Microbiology

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A mass airflow cabinet for handling and housing of laboratory rodents has been developed and tested. The unit consists of a high-efficiency particulate air filter and uniform distribution of air at a vertical velocity of 19 cm per s. Animals are maintained without bedding in mesh-bottomed cages that rest on rollers for rotation inside the cabinet. There is an air barrier of 90 cm per s separating the cabinet air from room air. Sampling for airborne bacteria yielded an average of 0.03 colony-forming units (CFU) per ft3 of air inside the cabinet, whereas 28.8 CFU per ft8 was simultaneously detected outside the cabinet during housekeeping, a reduction of almost three logs. The efficiency of the air barrier was tested by aerosolization of T3 phage. When phage was aerosolized 5 cm outside the cabinet, no phage could be detected 5 cm inside when the fans were operating; with the fans off an average of 1.6 x 101 plaque-forming units (PFU) per ft3 was

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Section: Resultsmentioning

confidence: 88%

“…Laminar air flow (LAF) and modifications of it have been applied to a variety of medical environments, including operating rooms (5, 9), tissue culture laboratories (7,10) and safety cabinets for handling hazardous biologicals (6,17).…”

mentioning

confidence: 99%

Mass Airflow Cabinet for Control of Airborne Infection of Laboratory Rodents

McGarrity¹,

Coriell²

1973

Applied Microbiology

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“…Dust-free room. The dust-free room (CRS Industries, Dresher, Pa.) was the same as previously reported (2,3). Air is passed through a high-efficiency particulate air (HEPA) filter that is 99.97% efficient for removal of dioctylphthalate particles having an average diameter of 0.3 Mm.…”

Section: Methodsmentioning

confidence: 99%

Medical Applications of Dust-free Rooms. III. Use in an Animal Care Laboratory

McGarrity¹,

Coriell²,

Schaedler³

et al. 1969

Applied Microbiology

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Bacterial air sampling in an animal care laboratory showed that dense aerosols are generated during cage changing and cage cleaning. Reyniers and Andersen sampling showed that the airborne bacteria numbered 50 to 200 colony-forming units (CFU)/ft3 of air. Of the viable particles collected by Andersen samplers, 78.5% were larger than 5.5 ,m. A low velocity laminar air flow system composed of high-efficiency particulate air (HEPA) filters and a ceiling distribution system maintained the number of airborne viable particles at low levels, generally less than 2 CFU/ft3. Vertical air flow of 15 ft/min significantly reduced the rate of airborne infection by a strain of Proteus mirabilis. Other factors shown to influence airborne infection included type of cage utilized, the use of bedding, the distance between cages, and the number of animals per cage. Accidental airborne infection in an animal care facility can influence the validity of experimental results and the economics of laboratory animal care (6). It is not an uncommon practice to maintain laboratory animals under observation for their normal life span, especially in toxicity

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“…The heart was then flushed with heparinized saline (3,000 units of heparin/L) till the effluent was clear. The surgery and cannulation were done in laminarflow clean air room 6. …”

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confidence: 99%