Medical applications of poly-4-hydroxybutyrate: a strong flexible absorbable biomaterial

Martin, David P.; Williams, Simon F.

doi:10.1016/s1369-703x(03)00040-8

Cited by 439 publications

(261 citation statements)

References 18 publications

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“…It has been found that in environments with fluctuating carbon levels, PHA producers have crucial advantages over rival species (14). In addition to their importance in the microbial world, these polymers have been studied for their potential uses in biodegradable consumer goods (12) and medical products (22) and as chemical precursors (4). Although many PHA monomers have been discovered, the most common are 3-hydroxyalkanoates (32).…”

mentioning

confidence: 99%

Roles of Multiple Acetoacetyl Coenzyme A Reductases in Polyhydroxybutyrate Biosynthesis in Ralstonia eutropha H16

Budde¹,

Mahan²,

Lu³

et al. 2010

J Bacteriol

116

104

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The bacterium Ralstonia eutropha H16 synthesizes polyhydroxybutyrate (PHB) from acetyl coenzyme A (acetyl-CoA) through reactions catalyzed by a ␤-ketothiolase (PhaA), an acetoacetyl-CoA reductase (PhaB), and a polyhydroxyalkanoate synthase (PhaC). An operon of three genes encoding these enzymatic steps was discovered in R. eutropha and has been well studied. Sequencing and analysis of the R. eutropha genome revealed putative isologs for each of the PHB biosynthetic genes, many of which had never been characterized. In addition to the previously identified phaB1 gene, the genome contains the isologs phaB2 and phaB3 as well as 15 other potential acetoacetyl-CoA reductases. We have investigated the roles of the three phaB isologs by deleting them from the genome individually and in combination. It was discovered that the gene products of both phaB1 and phaB3 contribute to PHB biosynthesis in fructose minimal medium but that in plant oil minimal medium and rich medium, phaB3 seems to be unexpressed. This raises interesting questions concerning the regulation of phaB3 expression. Deletion of the gene phaB2 did not result in an observable phenotype under the conditions tested, although this gene does encode an active reductase. Addition of the individual reductase genes to the genome of the ⌬phaB1 ⌬phaB2 ⌬phaB3 strain restored PHB production, and in the course of our complementation experiments, we serendipitously created a PHB-hyperproducing mutant. Measurement of the PhaB and PhaA activities of the mutant strains indicated that the thiolase reaction is the limiting step in PHB biosynthesis in R. eutropha H16 during nitrogen-limited growth on fructose.

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mentioning

confidence: 99%

Roles of Multiple Acetoacetyl Coenzyme A Reductases in Polyhydroxybutyrate Biosynthesis in Ralstonia eutropha H16

Budde¹,

Mahan²,

Lu³

et al. 2010

J Bacteriol

116

104

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“…P4HB is currently evaluated for a number of medical applications. 17 In a first step, a 1.6% (w/v) polymer solution in chloroform was prepared to fabricate polymer minitubes with an inner diameter of 2.8 mm by dip-coating of stainless steel mandrels. The dip-coating procedure was performed in a repetitive, automated dipping process until a nominal wall thickness of 300 lm of the polymer tubes was achieved.…”

Section: Polymer Materials and Stent Prototype Manufacturementioning

confidence: 99%

A Biodegradable Slotted Tube Stent Based on Poly(l-lactide) and Poly(4-hydroxybutyrate) for Rapid Balloon-Expansion

et al. 2007

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Abstract-Safe vascular stent application requires rapid expansion of the stent to minimize the risk of procedural ischemia. While high expansion speeds can be achieved with metallic stents, they are not necessarily feasible with biodegradable polymeric stents due to the viscoelastic material behavior. This study reports on a novel biodegradable polymer blend material based on poly(L-lactide) (PLLA) and poly(4-hydroxybutyrate) (P4HB), and describes the mechanical properties and in vitro degradation behavior of a balloon-expandable slotted tube stent concept. The stent prototypes with nominal dimensions of 6.0 · 25 mm were manufactured by laser machining of solution cast PLLA/ P4HB tubes (I.D. = 2.8 mm, d = 300 lm). The stents were expanded within 1 min by balloon inflation to 8 bar, after 5 min preconditioning in 37°C water. Recoil and collapse pressure were 4.2% and 1.1 bar, respectively. During in vitro degradation collapse pressure initially increased to a maximum at 4 w and then decreased thereafter. After 48 w, molecular weight was decreased by 82%. In summary, the PLLA/P4HB slotted tube stents allowed for rapid balloonexpansion and exhibited adequate mechanical scaffolding properties suitable for a broad range of vascular and nonvascular applications.

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“…[1][2][3] Medical application of PHB and its copolymer, poly(hydroxyalkanoate) (PHA), has been investigated since the 1990s, and a PHA containing 4-hydroxybutyrate units was provisionally concluded to show higher biocompatibility and in vivo biodegradability than other PHAs. [4][5][6][7] In general, however, PHA materials exhibit few of the biological functions required of modern biomaterials. Many types of surface modifications of PHA films and fibrous mats, such as with collagen, C+ ion, chitosan, gelatin or hyaluronic acid, have therefore been used to enhance the biocompatibility and cell proliferation of PHB and PHA materials.…”

Section: Introductionmentioning

confidence: 99%

Dual biosyntheses of poly[(R)-3-hydroxybutyric acid] and silk protein for the fabrication of biofunctional bioplastic

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Doi

2011

Polym J

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This is the first report on dual synthesis of poly[(R)-3-hydroxybutyric acid] (PHB) and protein polymer by recombinant microorganisms for use in the fabrication of a biofunctional PHB-based material hybridized with functional proteins. PHB and recombinant silk protein, a model protein developed in this study, were synthesized by a recombinant Escherichia coli system, and then films of PHB hybridized with the silk protein (PHB/Silk) were prepared. The presence of the silk protein in the amorphous phase of the PHB/Silk film was demonstrated based on wide-angle X-ray diffraction and differential scanning calorimetry measurements, which also revealed the enhanced tensile strength and elongation at break of the PHB/Silk film. The cell adhesion of human mesenchymal stem cells (hMSCs) for the PHB/Silk film increased because of the addition of silk molecules to the film. This might have been because the silk molecules existed in the amorphous phase at the surface of the PHB/Silk films, and were exposed to hMSCs. The overall results illustrate the potential of this dual synthesis system as a versatile and useful platform method for preparation of biofunctional PHB-based materials, especially for biomedical applications.

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Medical applications of poly-4-hydroxybutyrate: a strong flexible absorbable biomaterial

Cited by 439 publications

References 18 publications

Roles of Multiple Acetoacetyl Coenzyme A Reductases in Polyhydroxybutyrate Biosynthesis in Ralstonia eutropha H16

Roles of Multiple Acetoacetyl Coenzyme A Reductases in Polyhydroxybutyrate Biosynthesis in Ralstonia eutropha H16

A Biodegradable Slotted Tube Stent Based on Poly(l-lactide) and Poly(4-hydroxybutyrate) for Rapid Balloon-Expansion

Dual biosyntheses of poly[(R)-3-hydroxybutyric acid] and silk protein for the fabrication of biofunctional bioplastic

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