Medpor® Acts on Stem Cells Derived from Peripheral Blood

Abstract

Porous polyethylene (PP or Medpor®) is an alloplastic material used worldwide for craniofacial reconstruction. Although several clinical studies are available, how this material alters osteoblast activity to promote bone formation is poorly understood. To study how PP can induce osteoblast differentiation in mesenchymal stem cells, the expression levels of bone related genes and mesenchymal stem cells marker were analyzed, using real time Reverse Transcription-Polymerase Chain Reaction. PP causes induction of osteoblast transcriptional factor RUNX2 and of the bone related genes osteocalcin (BGLAP) and alkaline phosphatase (ALPL). In contrast the expression of ENG was decreased in stem cells treated with PP respect to untreated cells, indicating the differentiation effect of this biomaterial on stem cells. The obtained results can be relevant to better understand the molecular mechanism of bone regeneration and as a model for comparing other materials with similar clinical effects.

<b>Analysis of indicators of osteogenesis, cytotoxicity and genotoxicity of an experimental β-TCP compared to other bone substitutes

“…The other bone substitutes used in this study correspond to the Bio-Oss ® and β-TCP (Bionnovation). Many studies have evaluated the behavior of Bio-Oss ® in which negative (Beloti et al, 2008;Bernhardt et al, 2011) and positive results were obtained in vivo and in vitro (Chaves et al, 2012;Kim et al, 2010;Liu et al, 2011;Sollazzo et al, 2010). In relation to the β-TCP (Bionnovation), no report in the literature was found.…”

“…The bovine hydroxyapatite (Bio-Oss ® ) has been commonly used as it is considered a biomaterial that has a predictable behavior due to its physicochemical properties. Bio-Oss ® has a porous nature that corresponds to 75 to 80% of its total volume, which greatly increases the surface area of the material and contributes to osteogenesis (Sollazzo et al, 2010) This bone substitute allows bone repair, since it is potentially osteoconductive and characterized for not activating the immune system (Bassi & Carvalho, 2011). It has been used to compare with other materials once many studies of this material have been performed with positive results (Chaves et al, 2012;Kim et al, 2010;Sollazzo et al, 2010).…”

Applications of Biomaterials for Bone Augmentation of Jaws: Clinical Outcomes and in Vitro Studies

“…Among the different type of alloplastic bone graft, PerioGlass ® (PG) (US Biomaterials Corp., Alachua, FL) is used to fill periodontal defects and rehabilitation of PTEJs, thanks to biocompatibility and osteoconductive properties 39 @ C I C E d i z i o n i I n t e r n a z i o n a l i (41)(42)(43). Moreover, promotes mineralized extracellular matrix deposition, enhancing bone formation.…”