Meeting report: First International Conference on Crimean-Congo hemorrhagic fever

Papa, Anna; Weber, Friedemann; Hewson, Roger; Weidmann, Manfred; Köksal, İftihar; Korukluoğlu, Gülay; Mirazimi, Alì

doi:10.1016/j.antiviral.2015.05.005

Cited by 46 publications

(33 citation statements)

References 34 publications

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“…Additional factors include the altitude, the land cover type, the transitional woodland/shrub land per person, and the number of livestock per person . Since no other CCHF case was detected in Greece, the high seroprevalence was attributed to the circulation of a strain with low pathogenicity, such as the AP92 strain which was isolated in 1975 from Rhipicephalus bursa ticks in Greece . This strain belongs to a distinct genetic lineage than the other pathogenic strains in Europe.…”

Section: Tick‐borne Viral Diseasesmentioning

confidence: 99%

Emerging arboviral human diseases in Southern Europe

Papa

2017

Journal of Medical Virology

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Southern Europe is characterized by unique landscape and climate which attract tourists, but also arthropod vectors, some of them carrying pathogens. Among several arboviral diseases that emerged in the region during the last decade, West Nile fever accounted for high number of human cases and fatalities, while Crimean-Congo hemorrhagic fever expanded its geographic distribution, and is considered as a real threat for Europe. Viruses evolve rapidly and acquire mutations making themselves stronger and naive populations more vulnerable. In an effort to tackle efficiently the emerging arboviral diseases, preparedness and strategic surveillance are needed for the early detection of the pathogen and containment and mitigation of probable outbreaks. In this review, the main human arboviral diseases that emerged in Southern Europe are described.

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Section: Tick‐borne Viral Diseasesmentioning

confidence: 99%

Emerging arboviral human diseases in Southern Europe

Papa

2017

Journal of Medical Virology

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“…Greece is a Mediterranean country where currently only one CCHF case has been reported (Papa et al, 2015a). The absence of CCHF cases in Greece, together with the high seroprevalence in the human population, and the fact that one of the veterinarians who isolated the AP92 strain demonstrated very high titres of anti-CCHFV antibodies, led to the suggestion that the AP92 strain is not pathogenic for humans (Papa et al, 2015b). Besides the prototype AP92 strain, a novel AP92-like strain (KF146306) has been recently detected in Greece in R. bursa collected from sheep in an area with 6% CCHFV seroprevalence (Papa et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

Combination of RT-PCR and proteomics for the identification of Crimean-Congo hemorrhagic fever virus in ticks

Mera

Chaligiannis

Hernández-Jarguín

et al. 2017

Heliyon

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Crimean-Congo hemorrhagic fever (CCHF) is an emerging tick-borne zoonotic disease caused by the CCHF virus (CCHFV). In this study, an experimental approach combining RT-PCR and proteomics was used for the identification and characterization of CCHFV in 106 ticks from 7 species that were collected from small ruminants in Greece. The methodological approach included an initial screening for CCHFV by RT-PCR followed by proteomics analysis of positive and control negative tick samples. This novel approach allowed the identification of CCHFV-positive ticks and provided additional information to corroborate the RT-PCR findings using a different approach. Two ticks, Dermacentor marginatus and Haemaphysalis parva collected from a goat and a sheep, respectively were positive for CCHFV. The sequences for CCHFV RNA segments S and L were characterized by RT-PCR and proteomics analysis of tick samples, respectively. These results showed the possibility of combining analyses at the RNA and protein levels using RT-PCR and proteomics for the characterization of CCHFV in ticks. The results supported that the CCHFV identified in ticks are genetic variants of the AP92 strain. Although the AP92-like strains probably do not represent a high risk of CCHF to the population, the circulation of genetically diverse CCHFV strains could potentially result in the appearance of novel viral genotypes with increased pathogenicity and fitness.

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“…Many factors, such as climatic changes, geographical conditions, livestock sector, and the number of people who have contacted ticks, affect the prevalence of the CCHF. The genetic variability of CCHFV results in the need for new diagnostic tests and suitable therapeutic agents to fight against the disease . The cause of the clinical difference between the cases despite the similarities between the virus strains detected in Turkey cannot be explained .…”

Section: Discussionmentioning

confidence: 99%

HULC and 7SL RNA expression levels in patients with Crimean‐Congo hemorrhagic fever

Bayyurt

Arslan

Engin

et al. 2018

Journal of Medical Virology

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Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne disease caused by the Crimean-Congo hemorrhagic fever virus. Long non-coding RNAs (lncRNAs) are generally classified as transcripts longer than 200 nucleotides (nt). The various lncRNAs expressed in infected cells are responsible for regulating the expression of viral and host genes. This is the first study to investigate hepatocellular carcinoma upregulated long non-coding RNA (HULC) and 7SL RNA expression levels in patients with CCHF. Blood samples were taken from 100 individuals (60 patients and 40 controls), and total RNA isolation was performed. Quantitative polymerase chain reaction (qPCR) was performed using the SYBR Green method to determine HULC and 7SL RNA expression levels in the study population. Compared the patient and control groups, HULC was upregulated statistically significantly (P = 0.04) and 7SL RNA was downregulated (P = 0.93) in patients. Also, there was a statistically significant difference between fatal cases and surviving patients for HULC and 7SL RNA (P < 0.01 and P = 0.03, respectively). In addition, HULC expression was increased statistically significantly in fatal cases compared with surviving patients in terms of clinical parameters such as aspartate aminotransferase (P < 0.01), alanine aminotransferase (P < 0.01), international normalized ratio (P = 0.05), prothrombin time (P = 0.01), active partial thromboplastin time (P < 0.01), and lactate dehydrogenase (P < 0.01). These findings highlighted that HULC and 7SL RNA could be important mediators for studying the pathogenesis of CCHF and significant therapeutic targets of the disease.

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Meeting report: First International Conference on Crimean-Congo hemorrhagic fever

Cited by 46 publications

References 34 publications

Emerging arboviral human diseases in Southern Europe

Emerging arboviral human diseases in Southern Europe

Combination of RT-PCR and proteomics for the identification of Crimean-Congo hemorrhagic fever virus in ticks

HULC and 7SL RNA expression levels in patients with Crimean‐Congo hemorrhagic fever

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