Membrane Biogenesis of Subunit II of Cytochrome bo Oxidase: Contrasting Requirements for Insertion of N-terminal and C-terminal Domains

Celebi, Nil; Liang, Yi; Facey, Sandra J.; Kühn, Andreas; Dalbey, Ross E.

doi:10.1016/j.jmb.2006.01.030

Cited by 77 publications

(72 citation statements)

References 40 publications

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“…3D). Like procoat, CyoA-N-P2 inserts across the membrane by the YidC-only pathway (2). As a control, we showed that the membrane insertion of C-Lep (Fig.…”

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confidence: 70%

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Isolation of Cold-Sensitive yidC Mutants Provides Insights into the Substrate Profile of the YidC Insertase and the Importance of Transmembrane 3 in YidC Function

2007

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YidC, a 60-kDa integral membrane protein, plays an important role in membrane protein insertion in bacteria. YidC can function together with the SecYEG machinery or operate independently as a membrane protein insertase. In this paper, we describe two new yidC mutants that lead to a cold-sensitive phenotype in bacterial cell growth. Both alleles impart a cold-sensitive phenotype and result from point mutations localized to the third transmembrane (TM3) segment of YidC, indicating that this region is crucial for YidC function. We found that the yidC(C423R) mutant confers a weak phenotype on membrane protein insertion while a yidC(P431L) mutant leads to a stronger phenotype. In both cases, the affected substrates include the Pf3 coat protein and ATP synthase F 1 F o subunit c (F o C), while CyoA (the quinol binding subunit of the cytochrome bo3 quinol oxidase complex) and wild-type procoat are slightly affected or not affected in either cold-sensitive mutant. To determine if the different substrates require various levels of YidC activity for membrane insertion, we performed studies where YidC was depleted using an arabinose-dependent expression system. We found that ؊3M-PC-Lep

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“…3D). Like procoat, CyoA-N-P2 inserts across the membrane by the YidC-only pathway (2). As a control, we showed that the membrane insertion of C-Lep (Fig.…”

mentioning

confidence: 70%

“…CyoA-N-P2 corresponds to the amino-terminal part of CyoA fused to the Lep soluble P2 domain (2) ( Table 1). Both of these constructs insert into the membrane by the YidC pathway (2,17). Membrane insertion of PC-Lep and CyoA-N-P2 was monitored by signal peptidase processing ( Fig.…”

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confidence: 99%

Isolation of Cold-Sensitive yidC Mutants Provides Insights into the Substrate Profile of the YidC Insertase and the Importance of Transmembrane 3 in YidC Function

2007

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“…Subunit a of the cytochrome oxidase, CyoA, has also been shown to require both YidC and SecYEG for insertion in vitro (12). Based on the individual insertion requirements of constructs containing either the Nor C-terminal parts of CyoA, it has been suggested that SecYEG and YidC work sequentially in the insertion of CyoA with YidC inserting the N-terminal hairpin and SecYEG inserting the C-terminal TMS and long periplasmic C-terminal tail (13,46). This strict sequential insertion is only alleviated when a long linker between TMSs 1 and 2 is introduced, after which the C terminus can insert independently of the N-terminal hairpin (39).…”

Section: Discussionmentioning

confidence: 99%

Conserved Negative Charges in the Transmembrane Segments of Subunit K of the NADH:Ubiquinone Oxidoreductase Determine Its Dependence on YidC for Membrane Insertion

Driessen

2010

Journal of Biological Chemistry

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All members of the Oxa1/Alb3/YidC family have been implicated in the biogenesis of respiratory and energy transducing proteins. In Escherichia coli, YidC functions together with and independently of the Sec system. Although the range of proteins shown to be dependent on YidC continues to increase, the exact role of YidC in insertion remains enigmatic. Here we show that YidC is essential for the insertion of subunit K of the NADH: ubiquinone oxidoreductase and that the dependence is due to the presence of two conserved glutamate residues in the transmembrane segments of subunit K. The results suggest a model in which YidC serves as a membrane chaperone for the insertion of the less hydrophobic, negatively charged transmembrane segments of NuoK.

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“…However, depletion of YidC in vivo only slightly affects the levels of these Secdependent IMPs, indicating that it is not essential for insertion. YidC can also act upstream of the Sec translocon, as demonstrated for the lipoprotein CyoA (subunit II of the cytochrome bo 3 oxidase) (4,13,45). YidC is sufficient to catalyze insertion of the N-terminal domain of CyoA while translocation of the large C-terminal domain requires the Sec translocon.…”

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confidence: 99%

Role for Escherichia coli YidD in Membrane Protein Insertion

Lavèn

Klepsch

et al. 2011

J Bacteriol

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YidC has an essential but poorly defined function in membrane protein insertion and folding in bacteria. The yidC gene is located in a gene cluster that is highly conserved in Gram-negative bacteria, the gene order being rpmH, rnpA, yidD, yidC, and trmE. Here, we show that Escherichia coli yidD, which overlaps with rnpA and is only 2 bp upstream of yidC, is expressed and localizes to the inner membrane, probably through an amphipathic helix. Inactivation of yidD had no discernible effect on cell growth and viability. However, compared to control cells, ⌬yidD cells were affected in the insertion and processing of three YidC-dependent inner membrane proteins. Furthermore, in vitro cross-linking showed that YidD is in proximity of a nascent inner membrane protein during its localization in the Sec-YidC translocon, suggesting that YidD might be involved in the insertion process.

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Membrane Biogenesis of Subunit II of Cytochrome bo Oxidase: Contrasting Requirements for Insertion of N-terminal and C-terminal Domains

Cited by 77 publications

References 40 publications

Isolation of Cold-Sensitive yidC Mutants Provides Insights into the Substrate Profile of the YidC Insertase and the Importance of Transmembrane 3 in YidC Function

Isolation of Cold-Sensitive yidC Mutants Provides Insights into the Substrate Profile of the YidC Insertase and the Importance of Transmembrane 3 in YidC Function

Conserved Negative Charges in the Transmembrane Segments of Subunit K of the NADH:Ubiquinone Oxidoreductase Determine Its Dependence on YidC for Membrane Insertion

Role for Escherichia coli YidD in Membrane Protein Insertion

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