Membrane-immobilized haptoglobin as affinity matrix for a hemoglobin-A1c immunosensor

Stöllner, Daniela; Stöcklein, Walter; Scheller, Frieder W.; Warsinke, Axel

doi:10.1016/s0003-2670(02)00779-1

Cited by 60 publications

(35 citation statements)

References 25 publications

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“…In a subsequent study, Stöllner et al and co-workers modified this biosensor for use as an amperometric immunosensor (Stöllner, Stöcklein, Scheller, & Warsinke, 2002). Their system works in 2 steps: selective enrichment of total hemoglobin on the surface of an affinity matrix followed by specific detection of immobilized HbA1c using a GOx-conjugated antiHbA1c antibody.…”

Section: Biosensors Based On Hba1cmentioning

confidence: 99%

“…They introduced more electrochemical techniques in this approach. A zirconium dioxide nanoparticle-modified pyrolytic graphite electrode (PGE) was used in the presence of didodecyldimethylammonium bromide (DDAB) for total hemoglobin immobilization rather than a haptoglobin-modified cellulose membrane on a www.intechopen.com The haptoglobin-modified cellulose membranes were fixed onto a Clark-type electrode (Stöllner, Stöcklein, Scheller, & Warsinke, 2002). platinum electrode.…”

Section: Biosensors Based On Hba1cmentioning

confidence: 99%

See 1 more Smart Citation

Electrochemical Biosensor for Glycated Hemoglobin (HbA1c)

Chen¹,

Pritzker²,

Sheikholeslam³

2011

Biosensors for Health, Environment and Biosecurity

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Section: Biosensors Based On Hba1cmentioning

confidence: 99%

Section: Biosensors Based On Hba1cmentioning

confidence: 99%

Electrochemical Biosensor for Glycated Hemoglobin (HbA1c)

Chen¹,

Pritzker²,

Sheikholeslam³

2011

Biosensors for Health, Environment and Biosecurity

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“…The H 2 O 2 generated in presence of glucose by GOD have also been used by Stçllner et al [68] for the development of an immunoassay for hemoglobyn-A1c, using a cellulose membrane as affinity matrix. In this case, the membrane is modified with an immunoreagent and all the incubation steps are performed in a reaction tube.…”

Section: Other Solid Supportsmentioning

confidence: 99%

Recent Advances in Electrochemical Enzyme Immunoassays

2005

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Enzyme immunoassays (EIAs) are currently the predominant analytical technique for the quantitative determination of a broad variety of analytes in clinical, medical, biotechnological, and environmental significance. Although the most common detection methods for EIAs are based on spectroscopic measurements, electrochemical techniques, due to their high sensitivity, selectivity, simplicity and low cost, have emerged as a very attractive alternative to carry out the detection step in this kind of assays. The intention of this review is to cover the progress and development in integrating electrochemical detection methods with EIAs, over the past five years.

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“…Currently, the quantitative analysis of HbA1c is performed with a variety of analytical techniques, including electrophoresis [4], ion-exchange chromatography [5], boronate affinity chromatography [6], mass spectrometry [7][8][9], immunoassays [10][11][12][13], electrochemical detection [14][15][16][17][18][19], piezoelectric sensors [20][21][22], chemiluminescence [23], surface plasmon resonance [24] and surface-enhanced resonance Raman spectroscopy [25]. Among these available techniques, the major drawbacks are that such methods are rather complicated, take a long time for the analysis [10] and require the use of labelled antibodies [16], expensive equipment [20,22] and/or sample preparation prior to the analysis [26].…”

Section: Introductionmentioning

confidence: 99%

Selective label-free electrochemical impedance measurement of glycated haemoglobin on 3-aminophenylboronic acid-modified eggshell membranes

et al. 2015

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We propose a novel alternative approach to long-term glycaemic monitoring using eggshell membranes (ESMs) as a new immobilising platform for the selective label-free electrochemical sensing of glycated haemoglobin (HbA1c), a vital clinical index of the glycaemic status in diabetic individuals. Due to the unique features of a novel 3-aminophenylboronic acid-modified ESM, selective binding was obtained via cis-diol interactions. This newly developed device provides clinical applicability as an affinity membrane-based biosensor for the identification of HbA1c over a clinically relevant range (2.3 - 14 %) with a detection limit of 0.19%. The proposed membrane-based biosensor also exhibited good reproducibility. When analysing normal and abnormal HbA1c levels, the within-run coefficients of variation were 1.68 and 1.83%, respectively. The run-to-run coefficients of variation were 1.97 and 2.02%, respectively. These results demonstrated that this method achieved the precise and selective measurement of HbA1c. Compared with a commercial HbA1c kit, the results demonstrated excellent agreement between the techniques (n = 15), demonstrating the clinical applicability of this sensor for monitoring glycaemic control. Thus, this low-cost sensing platform using the proposed membrane-based biosensor is ideal for point-of-care diagnostics.

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Membrane-immobilized haptoglobin as affinity matrix for a hemoglobin-A1c immunosensor

Cited by 60 publications

References 25 publications

Electrochemical Biosensor for Glycated Hemoglobin (HbA1c)

Electrochemical Biosensor for Glycated Hemoglobin (HbA1c)

Recent Advances in Electrochemical Enzyme Immunoassays

Selective label-free electrochemical impedance measurement of glycated haemoglobin on 3-aminophenylboronic acid-modified eggshell membranes

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