MEMS piezoresistive cantilever for the direct measurement of cardiomyocyte contractile force

Matsudaira, Kenei; Nguyen, Thanh-Vinh; Shoji, Kayoko Hirayama; Tsukagoshi, Takuya; Takahata, Tomoyuki; Shimoyama, Isao

doi:10.1088/1361-6439/aa8350

Cited by 25 publications

(24 citation statements)

References 15 publications

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“…Two electrodes were placed at the roots of the two hinges and the resistance of the cantilever is defined as the resistance between the two electrodes. The fabrication process of the cantilever can be found in previous studies [24][25][26][27][28][29]. A photograph of the fabricated sensor chip attached and wire-bonded to a flexible substrate and a photograph of the cantilever are shown in Figure 2a.…”

Section: Piezoresistive Cantilevermentioning

confidence: 99%

“…A differential pressure in the range of ±10 Pa was applied to the cantilever using a pressure generator. The fractional resistance change of the cantilever was measured using the setup reported in previous studies [24][25][26][27][28][29][30][31][32], which consists of the Wheatstone bridge circuit and an amplifying circuit whose output is recorded using a ScopeCorder (Yokogawa Test & Measurement Co., Tokyo, Japan, DL850). The calibration result in Figure 2c shows the linear relationship between the differential pressure and the fractional resistance change of the cantilever.…”

Section: Piezoresistive Cantilevermentioning

confidence: 99%

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MEMS-Based Pulse Wave Sensor Utilizing a Piezoresistive Cantilever

Nguyen

Mizuki

Tsukagoshi

et al. 2020

Sensors

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This paper reports on a microelectromechanical systems (MEMS)-based sensor for pulse wave measurement. The sensor consists of an air chamber with a thin membrane and a 300-nm thick piezoresistive cantilever placed inside the chamber. When the membrane of the chamber is in contact with the skin above a vessel of a subject, the pulse wave of the subject causes the membrane to deform, leading to a change in the chamber pressure. This pressure change results in bending of the cantilever and change in the resistance of the cantilever, hence the pulse wave of the subject can be measured by monitoring the resistance of the cantilever. In this paper, we report the sensor design and fabrication, and demonstrate the measurement of the pulse wave using the fabricated sensor. Finally, measurement of the pulse wave velocity (PWV) is demonstrated by simultaneously measuring pulse waves at two points using the two fabricated sensor devices. Furthermore, the effect of breath holding on PWV is investigated. We showed that the proposed sensor can be used to continuously measure the PWV for each pulse, which indicates the possibility of using the sensor for continuous blood pressure measurement.

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Section: Piezoresistive Cantilevermentioning

confidence: 99%

Section: Piezoresistive Cantilevermentioning

confidence: 99%

MEMS-Based Pulse Wave Sensor Utilizing a Piezoresistive Cantilever

Nguyen

Mizuki

Tsukagoshi

et al. 2020

Sensors

Self Cite

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“…EB-mediated cardiac differentiation suffers from variability of EB size, organoid heterogeneity, and poor spatial organization 26 . For example, in the biowire platform 27 , microfluidic-based devices 28 , and cantilever systems 29 , cardiomyocytes were first differentiated from hiPSCs/hESCs in conventional tissue culture plates and then seeded as single cells or tissue clusters into the fabricated devices to form adult-like aligned cardiac muscle 23,30 . The advantages of these engineered cardiac tissue models are not only the structural mimicry of adult heart tissue, but also their capability of electromechanical measurements and stimulations 31,32 .…”

Section: Introductionmentioning

confidence: 99%

Generation of spatial-patterned early-developing cardiac organoids using human pluripotent stem cells

et al. 2018

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The discovery of human induced pluripotent stem cells (hiPSCs) has provided an unprecedented opportunity to study tissue morphogenesis and organ development as “organogenesis-in-a-dish”. Current approaches of cardiac organoid engineering rely on either direct cardiac differentiation from embryoid bodies or generation of aligned cardiac tissues from pre-differentiated cardiomyocytes from monolayer hiPSCs. To experimentally model early cardiac organogenesis in vitro, our protocol combines biomaterials-based cell patterning with stem cell organoid engineering. Three-dimensional cardiac microchambers are created from two-dimensional hiPSC colonies, which approximates an early developing heart with distinct spatial organization and self-assembly. With proper training on photolithography microfabrication, maintenance of human pluripotent stem cells, and cardiac differentiation, a graduate student with guidance will likely be able to carry out this experimental protocol, which requires approximately three weeks. We envisage that this in vitro model of human early heart development could serve as an embryotoxicity screening assay in drug discovery, regulation, and prescription for healthy fetal development. Currently, this protocol has yet to be implemented to assess the cardiac microchamber formation from the hiPSC lines with inherited disease, which can potentially be used to study the disease mechanisms of cardiac malformations at early stage of embryogenesis.

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“…The design flexibility of MEMS translates into the possibility of force measurements along multiple axes, coupled to unprecedented measurable force range (10 −12 ÷ 10 −3 N; Sun and Nelson, 2007 ). Notable examples of MEMS devices for cell biomechanical characterization are represented by the work of Matsudaira et al (2017) who developed a silicon piezoresistive cantilever platform for measuring the beating contraction force of iPS-derived cardiomyocytes, and that of Takahashi et al, 2016 who designed a MEMS force plate to measure single cell horizontal and vertical traction forces. Although the majority of these devices are passive, approaches to single-cell mechanical actuation using MEMS have to be acknowledged ( Scuor et al, 2006 ; Antoniolli et al, 2014 ), also integrating force sensing capabilities besides actuation ones ( Fior et al, 2011 ; Zhang and Dong, 2012 ).…”

Section: Microengineered Platformsmentioning

confidence: 99%

Biomechanical Characterization at the Cell Scale: Present and Prospects

et al. 2018

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The rapidly growing field of mechanobiology demands for robust and reproducible characterization of cell mechanical properties. Recent achievements in understanding the mechanical regulation of cell fate largely rely on technological platforms capable of probing the mechanical response of living cells and their physico–chemical interaction with the microenvironment. Besides the established family of atomic force microscopy (AFM) based methods, other approaches include optical, magnetic, and acoustic tweezers, as well as sensing substrates that take advantage of biomaterials chemistry and microfabrication techniques. In this review, we introduce the available methods with an emphasis on the most recent advances, and we discuss the challenges associated with their implementation.

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MEMS piezoresistive cantilever for the direct measurement of cardiomyocyte contractile force

Cited by 25 publications

References 15 publications

MEMS-Based Pulse Wave Sensor Utilizing a Piezoresistive Cantilever

MEMS-Based Pulse Wave Sensor Utilizing a Piezoresistive Cantilever

Generation of spatial-patterned early-developing cardiac organoids using human pluripotent stem cells

Biomechanical Characterization at the Cell Scale: Present and Prospects

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