Mercury (II) removal by resistant bacterial isolates and mercuric (II) reductase activity in a new strain of Pseudomonas sp. B50A

Giovanella, Patrícia; Cabral, Lucélia; Bento, Fátima Menezes; Gianello, Clésio; Camargo, Flávio Anastácio de Oliveira

doi:10.1016/j.nbt.2015.05.006

Cited by 73 publications

(34 citation statements)

References 37 publications

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“…The volatilization rate then stabilized over 96% after 16 h, demonstrating the excellent Hg volatilization efficiency of the strain DC‐B2. The Hg(II) volatilization capacity was relatively higher than that of other Hg‐resistant bacteria reported previously …”

Section: Resultsmentioning

confidence: 53%

“…Some microorganisms may gradually evolve resistance towards Hg‐contaminated environments for their long‐term survival. The mechanisms promoting this resistance typically involve positive regulation of the mer operon, which allows for detoxification of Hg through the transformation of Hg(II) to the less toxic and relatively inert volatile Hg 0 …”

Section: Resultsmentioning

confidence: 99%

“…The mechanisms promoting this resistance typically involve positive regulation of the mer operon, which allows for detoxification of Hg through the transformation of Hg(II) to the less toxic and relatively inert volatile Hg 0 . 5,15,33 Furthermore, DC-B2 also displayed considerable resistance to Cr 6+ , Cu 2+ , Zn 2+ , Pb 2+ and As 3+ , with corresponding MICs of 350.0, 159.8, 104.1, 265.2 and 99.9 mg L −1 , and EC 50 values of 299.7, 107.9, 48.5, 205.5 and 63.2 mg L −1 , respectively ( Table 2). These results suggested that this strain has the potential to be used for the remediation of Hg-contaminated sites with complex metal contaminants.…”

Section: Identification Of Dc-b2mentioning

confidence: 99%

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Isolation of the Hg(II)‐volatilizing Bacillus sp. strain DC‐B2 and its potential to remediate Hg(II)‐contaminated soils

Chen

Dong

Shen

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND Mercury (Hg) contamination is a major global environmental problem, and its remediation using environmentally friendly biotechnologies has attracted increasing attention. In this study, an Hg(II)‐resistant bacterial strain, Bacillus sp. DC‐B2, was isolated from a metal‐contaminated site, and its ability to remediate five types of Hg‐contaminated soils was investigated. RESULTS The isolate DC‐B2 showed strong resistance to multiple metals and volatilized 91.6% of the Hg(II) in a culture with an initial Hg(II) concentration of 10 mg L−1 within 8 h. Microcosm experiments showed that the addition of DC‐B2 culture improved Hg(II) removal by 11.7–17.2% from four artificially Hg(II)‐spiked soil samples with initial Hg contents of approximately 100 mg kg−1 after incubating for 30 days under flooded conditions compared to the control treatment. For the field‐contaminated farmland soil containing 2.50 mg Hg kg−1, an Hg(II) elimination rate of 82.1% was achieved within 30 days with the DC‐B2 inoculation, relative to the 34.1% elimination rate observed for the uninoculated control. The amendment of peptone broth slightly inhibited Hg removal (2.2–8.7%). The remediation effectiveness was verified by observations of longer root and shoot lengths of lettuce plants grown in treated soils compared to those grown in the control soil. CONCLUSION The isolate Bacillus sp. DC‐B2 shows great potential to be developed as a bioagent for effective bioremediation of Hg‐contaminated soils. © 2018 Society of Chemical Industry

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Section: Resultsmentioning

confidence: 53%

Section: Resultsmentioning

confidence: 99%

Section: Identification Of Dc-b2mentioning

confidence: 99%

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Isolation of the Hg(II)‐volatilizing Bacillus sp. strain DC‐B2 and its potential to remediate Hg(II)‐contaminated soils

Chen

Dong

Shen

et al. 2019

J of Chemical Tech & Biotech

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“…One of the possible mechanisms may be mercuric reductase activity. The pH optimum for activit y of the enzyme is 8 [27], what corresponds with pH value favourable for bacteria growth, which was observed while incubation of investigated isolates (Tab. 2).…”

Section: Discussionmentioning

confidence: 68%

Isolation, screening and identification of mercury resistant bacteria from mercury contaminated soil

et al. 2016

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Abstract. New bacterial strains resistant to high concentration of mercury were obtained and character iz ed focusing on their potential application in bioremediation. The biological material was isolated from soil contaminated with mercury. The ability to removal of Hg from the liquid medium and the effect of the various pH and mercury concentrations in the environment on bacterial strains growth kinetics were tested. The selected strains were identified by analysis of the 16S ribosome subunit coding sequenc es as Pseudomonas syringae. The analysis of Hg concentration in liquid medium as effect of microb ial metabolism demonstrated that P. syringae is able to remove almost entire metal from mediu m after 120 hours of incubation. Obtained results revealed new ability of the isolated strain P. syrin ga e . Analyzed properties of this soil bacteria species able to reduce concentration of Hg or immob i lize this metal are promising for industrial wastewater treatment and bioremediation of the soils pollu t ed especially by mercury lamps scrapping, measuring instruments, dry batteries, detonators or burning fuels made from crude oil, which may also contain mercury. Selected bacteria strains provide efficient and relatively low-cost bioremediation of the areas and waters contaminated with Hg.

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“…The stationary phase is shown at incubation time of 32 hours to 40 hours. In this phase the number of cell populations remains due to the number of cells growing equal to the number of dead cells (Pelczar, and Chan, 2010); Giovanella, Cabral, Bento, Gianello, & Camargo, 2016;Waluyo, 2008). At incubation time of 44 hours, there is a decrease of OD (Optical Density) value so that at 44 hours incubation time is called death phase.…”

Section: Growth Curve Bacillus Spmentioning

confidence: 99%

Biochemical Properties of Mercuric Reductase from Local Isolate of Bacillus sp for Bioremediation Agent

Purkan

Nuzulla

Hadi

et al. 2017

Molekul

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Mercuric reductase is the important enzyme which catalyzes a reduction of a toxic Hg 2+ to non-toxic Hg 0 . The enzyme which has been potentially used as mercury bioremediation agent is produced by mercury resistant bacteria. These research aims are to determinate the resistance level of a local Bacillus sp to HgCl 2 in media, to determine the mercuric reductase activity from the bacteria, and to determine the biochemical properties of the mercuric reductase. The Bacillus sp was grown in the Nutrient Broth media with various of 0; 20; 40; 60; 120; and 160 µM HgCl 2 to know the response of the bacteria against mercury, The cell growth of Bacillus sp was measured by optical density (OD) method of at λ 600 nm. The mercuric reductase activity was assayed in the solution of MRA (Mercury Reductase Assay), then the oxidized NADPH was observed by the spectrophotometry method at λ340 nm. The result showed that the Bacillus sp has been resistant to media containing mercury at 120 µM, but the microbial growth was decreased by 50% in media containing mercury 80 µM. The Bacillus sp could produce highly the mercuric reductase enzyme at 16 hours of growth time with enzyme activity as 0.574 Unit/µg. The mercuric reductase from the bacteria has an optimum activity at pH 6 and temperature 37 °C

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Mercury (II) removal by resistant bacterial isolates and mercuric (II) reductase activity in a new strain of Pseudomonas sp. B50A

Cited by 73 publications

References 37 publications

Isolation of the Hg(II)‐volatilizing Bacillus sp. strain DC‐B2 and its potential to remediate Hg(II)‐contaminated soils

Isolation of the Hg(II)‐volatilizing Bacillus sp. strain DC‐B2 and its potential to remediate Hg(II)‐contaminated soils

Isolation, screening and identification of mercury resistant bacteria from mercury contaminated soil

Biochemical Properties of Mercuric Reductase from Local Isolate of Bacillus sp for Bioremediation Agent

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