Mesenchymal Stem Cells: Engineering Regeneration

Behfar, Atta; Terzic, André

doi:10.1111/j.1752-8062.2008.00024.x

Cited by 12 publications

(12 citation statements)

References 15 publications

(40 reference statements)

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“…A low density cardiopoietic stem cell culture (1,500 cells/cm 2 ) placed in the cardiogenic cocktail yields a 10, 30, and 65% population of cardiomyocytes by 3, 6, and 10 days, respectively [41]. In this way, cardiopoiesis enables targeted generation of lineage-specified stem cells [54]. …”

Section: Cardiopoiesis Fundamentalsmentioning

confidence: 99%

“…4). A cardiogenic cocktail-rich milieu can guide patient-derived adult stem cells to acquire a repair potential associated with cardiac transcription factor expression [40,54]. Adult stem cells suffering from sequestered plasticity are resuscitated by priming with recombinant factors TGF-β, BMP-4, Activin-A, IGF-1, IL-6, FGF-2, thrombin, and retinoic acid that mimic signals and pathways activated in natural cardiogenesis.…”

Section: Translating Cardiopoiesismentioning

confidence: 99%

“…Of note, however, the biological outcome of cardiopoiesis applied to an adult stem cell population should be distinguished from that of pluripotent counterparts as it intends to achieve a regenerative paracrine function in the heart rather than to recapitulate embryonic cardiomyogenesis [40,48]. The first clinically tested example of such an approach is lineage specification through conditioning of bone marrow derived mesenchymal stem cells from patients with ischemic heart failure to yield the cardiopoietic stem cell phenotype [40,41,54]. In mesenchymal stem cells, simultaneous activation with TGF-β, BMP-4 and Activin-A along with retinoic acid induces cytosolic expression of cardiac transcription factors, while IGF-1 and IL-6 prompt their nuclear translocation (Fig.…”

Section: Translating Cardiopoiesismentioning

confidence: 99%

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Stem cell therapy for heart failure: Ensuring regenerative proficiency

Terzic

Behfar

2016

Trends in Cardiovascular Medicine

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Patient-derived stem cells enable promising regenerative strategies but display heterogenous cardiac reparative proficiency, leading to unpredictable therapeutic outcomes impeding practice adoption. Means to establish and certify the regenerative potency of emerging biotherapies are thus warranted. In this era of clinomics, deconvolution of variant cytoreparative performance in clinical trials offers an unprecedented opportunity to map pathways that segregate regenerative from non-regenerative states informing the evolution of cardioregenerative quality systems. A maiden example of this approach is cardiopoiesis-mediated lineage-specification developed to ensure regenerative performance. Successfully tested in pre-clinical and early clinical studies, the safety and efficacy of the cardiopoietic stem cell phenotype is undergoing validation in pivotal trials for chronic ischemic cardiomyopathy offering the prospect of a next-generation regenerative solution for heart failure.

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Section: Cardiopoiesis Fundamentalsmentioning

confidence: 99%

Section: Translating Cardiopoiesismentioning

confidence: 99%

Section: Translating Cardiopoiesismentioning

confidence: 99%

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Stem cell therapy for heart failure: Ensuring regenerative proficiency

Terzic

Behfar

2016

Trends in Cardiovascular Medicine

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“…Adult stem cells are a leading candidate for clinical application in regenerative medicine based on accessibility, autologous status, and favorable proliferative potential 37. While there is increased understanding of the regenerative potential of stem cells derived from adipose tissue and of resident stem cells linked to the innate repair potential of mature tissues, current experience primarily relies on bone marrow-derived stem cells.…”

Section: Adult Stem Cellsmentioning

confidence: 99%

“…For disorders where mesenchymal stem cells are not needed on an emergency basis, it may be preferable to culture-expand autologous mesenchymal stem cells prior to implantation in an effort to personalize cell-based therapy 42. Expansion offers the opportunity to produce a large pool of naïve stem cells, and derive progeny honed for lineage-specification away from multipotency and into tissue-restricted cytopoiesis 37. Derivation and characterization of specialized mesenchymal stem cell subpopulations, and their selective application to specific disease conditions, is an emerging strategy for enhanced therapeutic outcome.…”

Section: Adult Stem Cellsmentioning

confidence: 99%

Stem Cell Platforms for Regenerative Medicine

Nelson

Behfar

Yamada

et al. 2009

Clinical Translational Sci

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The pandemic of chronic degenerative diseases associated with aging demographics mandates development of effective approaches for tissue repair. As diverse stem cells directly contribute to innate healing, the capacity for de novo tissue reconstruction harbors a promising role for regenerative medicine. Indeed, a spectrum of natural stem cell sources ranging from embryonic to adult progenitors has been recently identifi ed with unique characteristics for regeneration. The accessibility and applicability of the regenerative armamentarium has been further expanded with stem cells engineered by nuclear reprogramming. Through strategies of replacement to implant functional tissues, regeneration to transplant progenitor cells or rejuvenation to activate endogenous self-repair mechanisms, the overarching goal of regenerative medicine is to translate stem cell platforms into practice and achieve cures for diseases limited to palliative interventions. Harnessing the full potential of each platform will optimize matching stem cell-based biologics with the diseasespecifi c niche environment of individual patients to maximize the quality of long-term management, while minimizing the needs for adjunctive therapy. Emerging discovery science with feedback from clinical translation is therefore poised to transform medicine offering safe and effective stem cell biotherapeutics to enable personalized solutions for incurable diseases.

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Tissue scaffolds for skin wound healing and dermal reconstruction

Zhong

Zhang

Lim

2010

WIREs Nanomed Nanobiotechnol

577

409

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One of the major applications of tissue-engineered skin substitutes for wound healing is to promote the healing of cutaneous wounds. In this respect, many important clinical milestones have been reached in the past decades. However, currently available skin substitutes for wound healing often suffer from a range of problems including wound contraction, scar formation, and poor integration with host tissue. Engineering skin substitutes by tissue engineering approach has relied upon the creation of three-dimensional scaffolds as extracellular matrix (ECM) analog to guide cell adhesion, growth, and differentiation to form skin-functional and structural tissue. The three-dimensional scaffolds can not only cover wound and give a physical barrier against external infection as wound dressing, but also can provide support both for dermal fibroblasts and the overlying keratinocytes for skin tissue engineering. A successful tissue scaffold should exhibit appropriate physical and mechanical characteristics and provide an appropriate surface chemistry and nano and microstructures to facilitate cellular attachment, proliferation, and differentiation. A variety of scaffolds have been fabricated based on materials ranging from naturally occurring ones to those manufactured synthetically. This review discusses a variety of commercial or laboratory-engineered skin substitutes for wound healing. Central to the discussion are the scaffolds/materials, fabrication techniques, and their characteristics associated with wound healing. One specifically highlighted emerging fabrication technique is electrospinning that allows the design and fabrication of biomimetic scaffolds that offer tremendous potential applications in wound healing of skin.

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Mesenchymal Stem Cells: Engineering Regeneration

Cited by 12 publications

References 15 publications

Stem cell therapy for heart failure: Ensuring regenerative proficiency

Stem cell therapy for heart failure: Ensuring regenerative proficiency

Stem Cell Platforms for Regenerative Medicine

Tissue scaffolds for skin wound healing and dermal reconstruction

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