Mesenchymal stem cells facilitate recovery from chemically induced liver damage and decrease liver fibrosis

Chang, Yao; Liu, Jen Wea; Lin, Po Cheng; Sun, Li; Peng, Chih Wen; Luo, Geng; Chen, Tse Min; Lee, Ru Ping; Lin, Shinn Zong; Harn, Horng Jyh; Chiou, Tzyy Wen

doi:10.1016/j.lfs.2009.08.003

Cited by 94 publications

(79 citation statements)

References 21 publications

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“…18,22,39 Different concentrations of growth factors including human hepatocyte growth factor (rhHGF), human fibroblast growth factor-4 (rhFGF-4) and EGF have been used to differentiate MSC into hepatocytes. 21,40 For assessing the differentiation of MSC to liver cells, hepatocytes marker genes such as ALB and α-fetoproteins analysed during in vitro differentiation. 5,37 ESC and MSC are two more common types of stem cells that are used to differentiate to hepatocytes.…”

Section: Rodents Models Of Human Liver Regenerationmentioning

confidence: 99%

“…A cell count of 1×10 6 suspended in 30µl of PBS or DMEM could be injected through the portal vein. 20,21,26 Homing and efficacy of cell therapy Histopathological methods such as Haematoxylin and Eosin (H&E), 22,28 Masson Trichrome 21,22 and Sirius Red 22 staining were used for detection of tissue structure changes after cell therapy in liver failure. The homing and engraftment of the transplanted cells to the target organ is key to the success of the cell therapy.…”

Section: Intrasplenicmentioning

confidence: 99%

“…15,16 Chemical induced damage Carbon tetrachloride (CCl 4 ) intoxication is a common method for induced liver injury in rodents that was originally described by Proctor and Chatamra. 17 Injection of doses between 0.5-1 mL/kg CCl4 dissolved in olive [18][19][20] or corn oil 5,21 twice a week for 4-8 weeks 18,22 have been used for liver injury in small animal models. 12,20 CCl4 can be injected to rodents intraperitoneally 12,23,24 or subcutaneously.…”

Section: Introductionmentioning

confidence: 99%

“…12,20 CCl4 can be injected to rodents intraperitoneally 12,23,24 or subcutaneously. 5,18,20,21 Paracetamol, 25 ally alcohol (AA) 26 and dimethylnitroseamine (DMN) are other chemical agents for inducing liver damage in rodents. 27 Mixture of Dgalactoseamine (D-gal) and lipopolysaccharide (LPS) 0.5 mg/g and 1 ng/g, respectively, in 1 ml of saline via intraperitoneal have also been used for inducing fulminant hepatic failure (FHF) in mice.…”

Section: Introductionmentioning

confidence: 99%

“…MSC isolated from adult tissues such as bone marrow, adipose tissue, umbilical cord blood and placenta can be differentiated into functional hepatocytes. 21 In recent studies the immunomodulatory effects of MSC have been documented. 34 MSC that play a critical role in the bone marrow niche have the ability to self-renew or differentiate to other lineages.…”

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confidence: 99%

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Methods of Liver Stem Cell Therapy in Rodents as Models of Human Liver Regeneration in Hepatic Failure

Goradel¹,

Darabi²,

Shamsasenjan³

et al. 2015

Adv Pharm Bull

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Section: Rodents Models Of Human Liver Regenerationmentioning

confidence: 99%

Section: Intrasplenicmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Methods of Liver Stem Cell Therapy in Rodents as Models of Human Liver Regeneration in Hepatic Failure

Goradel¹,

Darabi²,

Shamsasenjan³

et al. 2015

Adv Pharm Bull

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Mammalian MSC from selected species: Features and applications

et al. 2017

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Mesenchymal stromal/stem cells (MSC) are promising candidates for cellular therapy of different diseases in humans and in animals. Following the guidelines of the International Society for Cell Therapy, human MSC may be identified by expression of a specific panel of cell surface markers (CD105+, CD73+, CD90+, CD34-, CD14-, or CD11b-, CD79- or CD19-, HLA-DR-). In addition, multiple differentiation potential into at least the osteogenic, adipogenic, and chondrogenic lineage is a main criterion for MSC definition. Human MSC and MSC of a variety of mammals isolated from different tissues meet these criteria. In addition to the abovementioned, they express many more cell surface markers. Yet, these are not uniquely expressed by MSC. The gross phenotypic appearance like marker expression and differentiation potential is similar albeit not identical for MSC from different tissues and species. Similarly, MSC may feature different biological characteristics depending on the tissue source and the isolation and culture procedures. Their versatile biological qualities comprising immunomodulatory, anti-inflammatory, and proregenerative capacities rely largely on the migratory and secretory capabilities of MSC. They are attracted to sites of tissue lesion and secrete factors to promote self-repair of the injured tissue. This is a big perspective for clinical MSC applications in both veterinary and human medicine. Phase I/II clinical trials have been initiated to assess safety and feasibility of MSC therapies in acute and chronic disease settings. Yet, since the mode of MSC action in a specific disease environment is still unknown at large, it is mandatory to unravel the response of MSC from a given source onto a specific disease environment in suitable animal models prior to clinical applications. © 2017 International Society for Advancement of Cytometry.

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Targeting androgen receptor in bone marrow mesenchymal stem cells leads to better transplantation therapy efficacy in liver cirrhosis

et al. 2013

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Transplantation of bone marrow mesenchymal stem cells (BM‐MSCs) has been considered as an alternative therapy, replacing liver transplantation in clinical trials, to treat liver cirrhosis, an irreversible disease that may eventually lead to liver cancer development. However, low survival rate of the BM‐MSCs leading to unsatisfactory efficacy remains a major concern. Gender differences have been suggested in BM‐MSCs therapeutic application, but the effect of the androgen receptor (AR), a key factor in male sexual phenotype, in this application is not clear. Using two liver cirrhosis mouse models induced by CCl4 or thioacetamide, we showed that targeting AR in the BM‐MSCs improved their self‐renewal and migration potentials and increased paracrine effects to exert anti‐inflammatory and anti‐fibrotic actions to enhance liver repair. Mechanism dissection studies suggested that knocking out AR in BM‐MSCs led to improved self‐renewal and migration by alteration of the signaling of epidermal growth factor receptor and matrix metalloproteinase 9 and resulted in suppression of infiltrating macrophages and hepatic stellate cell activation through modulation of interleukin (IL)1R/IL1Ra signaling. Therapeutic approaches using either AR/small interfering RNA or the AR degradation enhancer, ASC‐J9®, to target AR in BM‐MSCs all led to increased efficacy for liver repair. Conclusion: Targeting AR, a key factor in male sexual phenotype, in BM‐MSCs improves transplantation therapeutic efficacy for treating liver fibrosis. (HEPATOLOGY 2013;57:1550–1563)

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Mesenchymal stem cells facilitate recovery from chemically induced liver damage and decrease liver fibrosis

Cited by 94 publications

References 21 publications

Methods of Liver Stem Cell Therapy in Rodents as Models of Human Liver Regeneration in Hepatic Failure

Methods of Liver Stem Cell Therapy in Rodents as Models of Human Liver Regeneration in Hepatic Failure

Mammalian MSC from selected species: Features and applications

Targeting androgen receptor in bone marrow mesenchymal stem cells leads to better transplantation therapy efficacy in liver cirrhosis

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