Metabolic acclimation of anammox consortia to decreased temperature

Huo, Tangran; Zhao, Yunpeng; Tang, Xi; Zhao, Huazhang; Ni, Shou‐Qing; Gao, Qiang; Liu, Sitong

doi:10.1016/j.envint.2020.105915

Cited by 69 publications

(29 citation statements)

References 80 publications

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“…When anammox consortia were subjected to starvation stress, upregulated expression of glycolysis/gluconeogenesis, pyruvate oxidation, and TCA cycle pathways could be observed in most of the abundant organisms (except that the pyruvate oxidation pathway was missing in MYX1), illustrating that these microbes tended to generate more intermediates for self-maintenance [ 46 ]. Relatively high degradation activity toward glycogen, a storage compound that could help organisms cope with substrate fluctuations, was observed in CHL1 and MYX2 [ 47 ].…”

Section: Resultsmentioning

confidence: 99%

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Response and resilience of anammox consortia to nutrient starvation

Wang

Liu

et al. 2022

Microbiome

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Background It is of critical importance to understand how anammox consortia respond to disturbance events and fluctuations in the wastewater treatment reactors. Although the responses of anammox consortia to operational parameters (e.g., temperature, dissolved oxygen, nutrient concentrations) have frequently been reported in previous studies, less is known about their responses and resilience when they suffer from nutrient interruption. Results Here, we investigated the anammox community states and transcriptional patterns before and after a short-term nutrient starvation (3 days) to determine how anammox consortia respond to and recover from such stress. The results demonstrated that the remarkable changes in transcriptional patterns, rather than the community compositions were associated with the nutritional stress. The divergent expression of genes involved in anammox reactions, especially the hydrazine synthase complex (HZS), and nutrient transportation might function as part of a starvation response mechanism in anammox bacteria. In addition, effective energy conservation and substrate supply strategies (ATP accumulation, upregulated amino acid biosynthesis, and enhanced protein degradation) and synergistic interactions between anammox bacteria and heterotrophs might benefit their survival during starvation and the ensuing recovery of the anammox process. Compared with abundant heterotrophs in the anammox system, the overall transcription pattern of the core autotrophic producers (i.e., anammox bacteria) was highly resilient and quickly returned to its pre-starvation state, further contributing to the prompt recovery when the feeding was resumed. Conclusions These findings provide important insights into nutritional stress-induced changes in transcriptional activities in the anammox consortia and would be beneficial for the understanding of the capacity of anammox consortia in response to stress and process stability in the engineered ecosystems.

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Section: Resultsmentioning

confidence: 99%

“…S 5 ). Interestingly, all abundant heterotrophs except for PLA2 showed unregulated ATP biosynthesis at S2 to maintain basic cellular reactions [ 46 ].…”

Section: Resultsmentioning

confidence: 99%

Response and resilience of anammox consortia to nutrient starvation

Wang

Liu

et al. 2022

Microbiome

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“…Jettenia caeni”, potentially giving “ Ca . B. fulgida” a metabolic advantage (Huo et al, 2020). This suggests that the upregulation of Csp proteins could be a promising mechanism of anammox adaptation to low temperatures.…”

Section: Resultsmentioning

confidence: 99%

“…B. sinica” and “ Ca . Jettenia caeni” (Huo et al, 2020); however, these proteins were unspecified. Despite these advances, a lot remains to be known about the various mechanisms of cold adaptation.…”

Section: Introductionmentioning

confidence: 99%

Adaptation of anammox bacteria to low temperature via gradual acclimation and cold shocks: distinctions in protein expression, membrane composition and activities

Kouba

Vejmelková

Zwolsman

et al. 2021

Preprint

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Anammox bacteria enable an efficient removal of nitrogen from sewage in processes involving partial nitritation and anammox (PN/A) or nitrification, partial denitrification, and anammox (N-PdN/A). In mild climates, anammox bacteria must be adapted to 15 C, typically by gradual temperature decrease; however, this takes months or years. To reduce the time necessary for the adaptation, an unconventional method of cold shocks is promising, involving hours-long exposure of anammox biomass to extremely low temperatures. We compared the efficacies of gradual temperature decrease and cold shocks to increase the metabolic activity of anammox (fed-batch reactor, planktonic Ca. Kuenenia). We assessed the cold shock mechanism on the level of protein expression (quantitative shot-gun proteomics, LC-HRMS/MS) and structure of membrane lipids (UPLC-HRMS/MS). The shocked culture was more active (0.66+-0.06 vs 0.48+-0.06 kg-N/kg-VSS/d) and maintained the relative content of N-respiration proteins at levels consistent levels with the initial state, whereas the content of these proteins decreased in gradually acclimated culture. Cold shocks also induced a more efficient up-regulation of cold shock proteins (e.g. CspB, TypA, ppiD). Ladderane lipids characteristic for anammox evolved to a similar end-point in both cultures which confirms their role in anammox bacteria adaptation to cold and indicates a three-pronged adaptation mechanism involving ladderane lipids (ladderane alkyl length, introduction of shorter non-ladderane alkyls, polar headgroup). Overall, we show the outstanding potential of cold shocks for low-temperature adaptation of anammox bacteria and provide yet unreported detailed mechanisms of anammox adaptation to low temperatures.

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“…"Brocadia" that dominate in reactors at low temperatures, down to 5-6 • C [reviewed in Tomaszewski et al (2017) and Cho et al (2020)]. They also maintain the activity in many freshwater environments at near 0 • C, which may indicate their cold-acclimation capacity (Huo et al, 2020 and the references therein). There are little data on the effect of low temperature on anammox bacteria of the genus Ca.…”

Section: Discussionmentioning

confidence: 99%

New Insight Into the Interspecies Shift of Anammox Bacteria Ca. “Brocadia” and Ca. “Jettenia” in Reactors Fed With Formate and Folate

et al. 2022

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The sensitivity of anaerobic ammonium-oxidizing (anammox) bacteria to environmental fluctuations is a frequent cause of reactor malfunctions. It was hypothesized that the addition of formate and folate would have a stimulating effect on anammox bacteria, which in turn would lead to the stability of the anammox process under conditions of a sharp increase in ammonium load, i.e., it helps overcome a stress factor. The effect of formate and folate was investigated using a setup consisting of three parallel sequencing batch reactors equipped with a carrier. Two runs of the reactors were performed. The composition of the microbial community was studied by the 16S rRNA gene profiling and metagenomic analysis. Among anammox bacteria, Ca. “Brocadia” spp. dominated during the first run. A stimulatory effect of folate on the daily nitrogen removal rate (dN) was identified. The addition of formate led to progress in dissimilatory nitrate reduction and stimulated the growth of Ca. “Jettenia” spp. The spatial separation of two anammox species was observed in the formate reactor: Ca. “Brocadia” occupied the carrier and Ca. “Jettenia”—the walls of the reactors. Biomass storage at low temperature without feeding led to an interspecies shift in anammox bacteria in favor of Ca. “Jettenia.” During the second run, a domination of Ca. “Jettenia” spp. was recorded along with a stimulating effect of formate, and there was no effect of folate on dN. A comparative genome analysis revealed the patterns suggesting different strategies used by Ca. “Brocadia” and Ca. “Jettenia” spp. to cope with environmental changes.

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Metabolic acclimation of anammox consortia to decreased temperature

Cited by 69 publications

References 80 publications

Response and resilience of anammox consortia to nutrient starvation

Response and resilience of anammox consortia to nutrient starvation

Adaptation of anammox bacteria to low temperature via gradual acclimation and cold shocks: distinctions in protein expression, membrane composition and activities

New Insight Into the Interspecies Shift of Anammox Bacteria Ca. “Brocadia” and Ca. “Jettenia” in Reactors Fed With Formate and Folate

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