Metabolic Engineering of Filamentous Fungi

Meyer, Vera

doi:10.1002/9783527823468.ch20

Cited by 13 publications

(10 citation statements)

References 167 publications

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“…Filamentous fungi have secretion capacities 10–1000 fold higher than that of bacterial, yeast, or mammalian hosts - generally limited to secretion titers lower than 10 g/L [ 211 ]. Despite this clear advantage, the production of heterologous proteins by filamentous fungi has faced many challenges that limit secretion titer.…”

Section: Bottlenecks and Bioengineering Strategiesmentioning

confidence: 99%

Unlocking the magic in mycelium: Using synthetic biology to optimize filamentous fungi for biomanufacturing and sustainability

Jo¹,

Zhang²,

Tam³

et al. 2023

Materials Today Bio

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Section: Bottlenecks and Bioengineering Strategiesmentioning

confidence: 99%

Unlocking the magic in mycelium: Using synthetic biology to optimize filamentous fungi for biomanufacturing and sustainability

Jo¹,

Zhang²,

Tam³

et al. 2023

Materials Today Bio

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“…These niches include extreme temperatures and pH, although fungal growth is confined at temperatures beyond 65 • C [44,45]. From different genome projects, it is obvious that filamentous fungi in general contain a great variety of plant biomass-degrading enzymes in their genomes [8,[46][47][48][49]. Furthermore, proteomic studies show that many of these enzymes are secreted when the fungi grow on lignocellulosic substrates [38,[50][51][52][53].…”

Section: Production Of Enzymes For Plant Biomass Utilizationmentioning

confidence: 99%

“…Among advantages are that titers obtained for secreted proteins, surpassing 10-1000-fold what can be achieved with secretion using bacterial, yeast or mammalian cells. In these cells, protein secretion titers are often less than a few g/L [8]. Unlike expression platforms using bacterial (especially E. coli) or yeast (e.g., Komagataella phaffii (syn.…”

Section: Recombinant Productionmentioning

confidence: 99%

“…Fungi have proven to be effective as industrial cell factories to produce a wide range of different products, due to their rapid growth, efficient utilization, and conversion of complex substrates into fermentable sugars. Among the most well-known ones are complex secondary metabolites such as antibiotics (e.g., penicillin), organic acids (e.g., citric acid) and various extracellular enzymes, including amylases and cellulases [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

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Fungal Cell Factories for Efficient and Sustainable Production of Proteins and Peptides

Lübeck

2022

Microorganisms

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Filamentous fungi are a large and diverse taxonomically group of microorganisms found in all habitats worldwide. They grow as a network of cells called hyphae. Since filamentous fungi live in very diverse habitats, they produce different enzymes to degrade material for their living, for example hydrolytic enzymes to degrade various kinds of biomasses. Moreover, they produce defense proteins (antimicrobial peptides) and proteins for attaching surfaces (hydrophobins). Many of them are easy to cultivate in different known setups (submerged fermentation and solid-state fermentation) and their secretion of proteins and enzymes are often much larger than what is seen from yeast and bacteria. Therefore, filamentous fungi are in many industries the preferred production hosts of different proteins and enzymes. Edible fungi have traditionally been used as food, such as mushrooms or in fermented foods. New trends are to use edible fungi to produce myco-protein enriched foods. This review gives an overview of the different kinds of proteins, enzymes, and peptides produced by the most well-known fungi used as cell factories for different purposes and applications. Moreover, we describe some of the challenges that are important to consider when filamentous fungi are optimized as efficient cell factories.

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“…Additionally, hosts such as A. niger, A. oryzae are classified as GRAS (generally recognized as safe) organisms, and therefore they are suitable for the industrial production of compounds destined to be used in humans. The most commonly employed hosts for the characterization of heterologous BGCs are A. nidulans and A. oryzae (Anyaogu and Mortensen, 2015;Meng et al, 2021;Meyer, 2021), but other species have been successfully developed into platform strains, as showcased in Table 1. In the following section, we will discuss the most relevant examples and highlight their major features.…”

Section: Filamentous Fungi As Platforms For the Heterologous Producti...mentioning

confidence: 99%

Transcriptional Activation of Biosynthetic Gene Clusters in Filamentous Fungi

Mózsik

Iacovelli

Bovenberg

et al. 2022

Front. Bioeng. Biotechnol.

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Filamentous fungi are highly productive cell factories, many of which are industrial producers of enzymes, organic acids, and secondary metabolites. The increasing number of sequenced fungal genomes revealed a vast and unexplored biosynthetic potential in the form of transcriptionally silent secondary metabolite biosynthetic gene clusters (BGCs). Various strategies have been carried out to explore and mine this untapped source of bioactive molecules, and with the advent of synthetic biology, novel applications, and tools have been developed for filamentous fungi. Here we summarize approaches aiming for the expression of endogenous or exogenous natural product BGCs, including synthetic transcription factors, assembly of artificial transcription units, gene cluster refactoring, fungal shuttle vectors, and platform strains.

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Metabolic Engineering of Filamentous Fungi

Cited by 13 publications

References 167 publications

Unlocking the magic in mycelium: Using synthetic biology to optimize filamentous fungi for biomanufacturing and sustainability

Unlocking the magic in mycelium: Using synthetic biology to optimize filamentous fungi for biomanufacturing and sustainability

Fungal Cell Factories for Efficient and Sustainable Production of Proteins and Peptides

Transcriptional Activation of Biosynthetic Gene Clusters in Filamentous Fungi

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