Metabolic Resistance in Abamectin-Resistant Bemisia tabaci Mediterranean from Northern China

Wang, Ran; Fang, Yong; Che, Wunan; Zhang, Qinghe; Wang, Jinda; Luo, Chen

doi:10.3390/toxins14070424

Cited by 13 publications

(11 citation statements)

References 37 publications

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“…The resistance inheritance of B. tabaci in previous studies mainly referred to autosomal, incompletely dominant or recessive inheritance. For example, inheritance of resistance to acetamiprid and buprofezin was autosomal and incompletely dominant, 54,55 and that of pyriproxyfen, cyantraniliprole, flupyradifurone, abamectin was autosomal and incompletely dominant 56–59 . In the current study, the inheritance of dinotefuran resistance is incompletely recessive and significant resistance to the other neonicotinoids was also observed.…”

Section: Discussionsupporting

confidence: 57%

“…For example, inheritance of resistance to acetamiprid and buprofezin was autosomal and incompletely dominant, 54,55 and that of pyriproxyfen, cyantraniliprole, flupyradifurone, abamectin was autosomal and incompletely dominant. [56][57][58][59] In the current study, the inheritance of dinotefuran resistance is incompletely recessive and significant resistance to the other neonicotinoids was also observed. Therefore, use of neonicotinoids should be avoided in the local area for a long time until resistance decreases to a low level.…”

Section: Discussionsupporting

confidence: 51%

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Resistance to dinotefuran in Bemisia tabaci in China: status and characteristics

Liu

Geng

et al. 2022

Pest Management Science

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BACKGROUND Bemisia tabaci (Gennadius) is a serious agricultural pest worldwide. Neonicotinoids are the most important new class of synthetic insecticides used in the management of B. tabaci. However, B. tabaci populations have developed resistance to various active ingredients in neonicotinoids following long‐term and widespread application. RESULTS Dinotefuran exhibited high toxicity against most B. tabaci field populations. One population (Din‐R) with a high level of resistance to dinotefuran (255.6‐fold) was first identified in the field. The Din‐R population exhibited medium to high levels of resistance to all the tested neonicotinoid insecticides and a high level of resistance to spinetoram. Genetic inheritance analysis revealed that resistance to dinotefuran was incompletely recessive and polygenic. The synergist piperonyl butoxide significantly increased the toxicity of dinotefuran to Din‐R. P450 activity in the Din‐R population was 2.19‐fold higher than in the susceptible population. RNA‐sequencing analysis showed that 12 P450 genes were significantly upregulated in the Din‐R population, of which CYP6DW5, CYP6JM1 and CYP306A1 were found to exhibit more than 3.00‐fold higher expression in Din‐R when using a reverse transcription quantitative real‐time polymerase chain reaction. Expression of eight P450 genes was obviously induced by dinotefuran, and CYP6DW5 showed the highest expression level. After knockdown of CYP6DW5 in Din‐R, the toxicity of dinotefuran increased significantly. CONCLUSION P450 had a crucial role in dinotefuran resistance in B. tabaci, and CYP6DW5 was involved in the resistance. These results provide important information for the management of resistance in B. tabaci and improve our understanding of the resistance mechanism of dinotefuran. © 2022 Society of Chemical Industry.

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Section: Discussionsupporting

confidence: 57%

Section: Discussionsupporting

confidence: 51%

Resistance to dinotefuran in Bemisia tabaci in China: status and characteristics

Liu

Geng

et al. 2022

Pest Management Science

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“…Therefore, B. tabaci has developed high resistance to various insecticides, such as cycloxaprid, abamectin, and pyriproxyfen. − IMI, as the most common neonicotinoid, is applied in whitefly control; however, the corresponding resistance has inevitably developed in whitefly and has been observed in many populations worldwide . In China, whitefly has become a severe and growing threat, as highly resistant populations are reported continuously. , Among these populations, the highest resistance ratio (RR) was observed in a whitefly population from Jiangsu province, with a value of 1900 …”

Section: Introductionmentioning

confidence: 99%

CYP6DW3 Metabolizes Imidacloprid to Imidacloprid-urea in Whitefly (Bemisia tabaci)

Xue

Huang

et al. 2023

J. Agric. Food Chem.

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Bemisia tabaci has developed high resistance to many insecticides and causes substantial agricultural and economic losses annually. The insecticide resistance of whitefly has been widely reported in previous studies; however, the underlying mechanism remains little known. In this study, we cloned two P450 genes: CYP6DW3 and CYP6DW5v1; these genes were markedly overexpressed in imidacloprid-resistant whitefly populations compared with susceptible populations, and knockdown of these genes decreased the imidacloprid resistance of whitefly. Moreover, heterologous expression of whitefly P450 genes in SF9 cells and metabolic studies showed that the CYP6DW3 protein could metabolize 14.11% imidacloprid and produced imidacloprid-urea in vitro. Collectively, the expression levels of CYP6DW3 and CYP6DW5v1 are positively correlated with imidacloprid resistance in B. tabaci. Our study further reveals that cytochrome P450 enzymes affect the physiological activities related to resistance in insects, which helps scholars more deeply understand the resistance mechanism, and contributes to the development of integrated pest management framework.

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“…Four populations of B. tabaci that were previously reported to be insecticide-resistant were tested for cross-resistance; these were an abamectin-resistant strain (XZ), an afidopyropen-resistant strain (HD-Afi), a cyantraniliprole-resistant strain (CYAN-R), and a flupyradifurone-resistant strain (FLU-SEL) (Wang et al, 2020a;Wang et al, 2020b;Wang et al, 2022b;Wang et al, 2022c). Populations of B. tabaci were collected from six Chinese provinces and tested for baseline susceptibility as previously described (Wang et al, 2022b). All field-collected populations were identified as Mediterranean (MED) cryptic species using a previously published method (Luo et al, 2002).…”

Section: Insectsmentioning

confidence: 99%

Toxicity, baseline of susceptibility, detoxifying mechanism and sublethal effects of chlorogenic acid, a potential botanical insecticide, on Bemisia tabaci

Wang

Zhang

et al. 2023

Front. Plant Sci.

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Bemisia tabaci is a threat to agriculture worldwide because of its potential to cause devastating damage to crops. Chlorogenic acid is a bioactive pesticidal phytochemical agent against various insect pests. We here determined the susceptibility of a laboratory strain of B. tabaci to chlorogenic acid and other popular insecticides, and the susceptibility of several field-collected populations to chlorogenic acid. Also, cross-resistance to four common insecticides was measured. Chlorogenic acid had the highest toxicity of all tested insecticides, and all the field-collected populations were susceptible to chlorogenic acid, and little cross-resistance was detected between chlorogenic acid and the other tested insecticides. Furthermore, analysis of enzyme activities and expression of P450 genes in B. tabaci after treatment with LC50 of chlorogenic acid suggested that enhanced P450 activity could be involved in chlorogenic acid detoxification. We subsequently evaluated sublethal effects of chlorogenic acid, and found that treatment with LC25 of chlorogenic acid prolonged duration of two developmental stages, reduced fecundity, and decreased survival rates of treated B. tabaci compared to untreated insects. Overall, these findings demonstrate strong toxicity and significant sublethal effects of chlorogenic acid on B. tabaci, and suggest that overexpression of P450 genes may be associated with chlorogenic acid detoxification.

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Metabolic Resistance in Abamectin-Resistant Bemisia tabaci Mediterranean from Northern China

Cited by 13 publications

References 37 publications

Resistance to dinotefuran in Bemisia tabaci in China: status and characteristics

Resistance to dinotefuran in Bemisia tabaci in China: status and characteristics

CYP6DW3 Metabolizes Imidacloprid to Imidacloprid-urea in Whitefly (Bemisia tabaci)

Toxicity, baseline of susceptibility, detoxifying mechanism and sublethal effects of chlorogenic acid, a potential botanical insecticide, on Bemisia tabaci

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