Metabolic responses of plant cell culture to hydrodynamic stress

Takeda, Takuya; Kitagawa, Takanori; Takeúchi, Yu; Seki, Minoru; Furusaki, Shintaro

doi:10.1002/cjce.5450760215

Cited by 25 publications

(9 citation statements)

References 43 publications

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“…Thus, for simplicity, a single rate was assumed for all cell lysis. While the specific rate of appearance of the TB dead cells was the only death constant during the first week, around 0.2 day −1 , contrary to Takeda et al (1998) reporting a neglectable cell lysis during 550-mL tank cultures of eucalyptus and safflower cells, lysis became an appreciable phenomenon later in the bioreactor batch culture, accounting for more than 20% of the total cell death. In fact, these differences might be mostly due to the characteristics of the cultured cells (cell line, culture age, and cultivation history) (Kieran et al, 1997) or the bioreactor scale.…”

Section: Discussioncontrasting

confidence: 81%

“…Programmed death was observed as a defense mechanism involving DNA cleavage upon pathogens infection (Mittler et al, 1996;Ryerson et al, 1996). Necrosis, on the other hand, is a passive phenomenon resulting from dramatic stresses in the cell environment (Dunlop et al, 1994;Kieran et al, 1995;MacLoughlin et al, 1998;Takeda et al, 1998). The consequence is a disappearance of cells from the tissue or the culture medium.…”

Section: Introductionmentioning

confidence: 99%

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Determination of growth and lysis kinetics in plant cell suspension cultures from the measurement of esterase release

Steward

Martin

Engasser

et al. 1999

Biotechnol. Bioeng.

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The death of Medicago sativa L. cells cultivated in a batch culture was investigated by measuring both the appearance of intact dead cells determined on the basis of the trypan blue (TB) dye exclusion, and the release of the cytoplasmic esterase activity into the culture medium upon cell death. Taking into account the strong instability of this released esterase activity, the total dead cell and lysed cell densities have been estimated. A mechanism for cell death and lysis is proposed and the specific rates of cell growth, death and lysis estimated. The specific rate of appearance of TB dead cells was low and essentially constant (0.25 day−1) during the first 8 days of the batch culture, and then increased above 1.5 day−1 after 2 weeks of cultivation. Whereas no lysis occurred during the first seven days, this phenomenon occurred during the second period and accounted for about 20% of the total cell death by the end of the process. Thus, the viability determined by the trypan blue exclusion method appeared to be invalid after 7 days of culture. When lysis of viable cells is taken into consideration, the specific growth rate was significantly increased and growth was shown to continue for a further 8 days. Increased sensitivity of the cells to shear stresses and consequent cell lysis could be the result of a 35% increase in the cell size © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 66: 114–121, 1999.

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Section: Discussioncontrasting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Determination of growth and lysis kinetics in plant cell suspension cultures from the measurement of esterase release

Steward

Martin

Engasser

et al. 1999

Biotechnol. Bioeng.

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“…However, the clear mechanism of hydrodynamic shear stress induced-damage to suspended plant cells is not well understood due to the diversity of cell lines, aggregate size distribution and cell morphologies, cell wall composition and culture age. Studies have reported that the cellular response of plant cells to hydrodynamic stress, including changes in cell viability (cell growth rate or membrane integrity), release of intracellular components (proteins or metabolites), changes in metabolism (OUR, mitochondrial activity, ATP concentration, cell wall composition, increase of calcium ions in cytoplasm) and changes in cell morphology and aggregation patterns [121,129,[133][134][135], are influenced by the intensity and the exposure duration of the cells to shear force.…”

Section: Shear Sensitivity Of Suspended Plant Cell Culturesmentioning

confidence: 99%

Bioreactor engineering for recombinant protein production in plant cell suspension cultures

Huang

McDonald

2009

Biochemical Engineering Journal

162

105

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“…Various approaches to measure the effect of shear stress on cell growth have been used; notably the viability of cells (Hooker et al, 1989;Zhong et al, 1994), and the production of secondary metabolites (Takeda et al, 1998;Zhong et al, 1994). More recent studies have focused on the cellular and molecular responses of plant cells to shear stress.…”

mentioning

confidence: 99%

Effects from shear stress on morphology and growth of early stages of Norway spruce somatic embryos

Sun

Aidun

Egertsdotter

2009

Biotech & Bioengineering

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The shear stress effect on directional expansion of pro embryogenic masses (PEMs) and suspensor cell development of somatic embryos of Norway spruce (Picea abies) at the proliferation stage was studied by a direct and quantitative image analysis system. The experimental system allowed for detailed observations of the effect of hydrodynamic shear stress in rotating and deforming liquid cultures of proliferating Norway spruce somatic embryos. Briefly, somatic embryos at an early development stage comprised only of clusters of meristematic cells without suspensor cells were fixed on an alginate film. The alginate film was affixed on the bottom of a flow cell and the somatic embryos were subjected to laminar flow through the chamber of the flow cell. Magnified images of the cell clusters were collected every 24 h. The image data was processed based on a normalized cross-correlation method, capable of measuring morphological and size features of individual cell clusters in both temporal and spatial domains. No suspensor cells developed in the cell clusters under shear stress of 140 s(-1) for the duration of the experiments. Cell clusters in the control cultured in stationary liquid conditions developed suspensor cells after 5-9 days in culture. Furthermore, the radial growth of meristematic cell clusters was inhibited by shear rates of 86 and 140 s(-1), corresponding to shear stress of 0.086 and 0.14 N/m(2), compared to growth under stationary conditions. The shear rate showed a significant negative correlation to growth rate. Control group showed no preference for direction during growth under static conditions.

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Metabolic responses of plant cell culture to hydrodynamic stress

Cited by 25 publications

References 43 publications

Determination of growth and lysis kinetics in plant cell suspension cultures from the measurement of esterase release

Determination of growth and lysis kinetics in plant cell suspension cultures from the measurement of esterase release

Bioreactor engineering for recombinant protein production in plant cell suspension cultures

Effects from shear stress on morphology and growth of early stages of Norway spruce somatic embryos

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