Metabolic syndrome and ADRB3 gene polymorphism in severely obese patients from South Italy

Abstract

Objective: To evaluate the prevalence of b 3 -adrenergic receptor (ADRB3) Trp64Arg polymorphism and its relationship with the metabolic syndrome in severe obesity. Design: Cross-sectional outpatients study. Patients and methods: In 265 (100 men) severely obese non-diabetic subjects and 78 (25 men) healthy volunteers, genomic DNA was isolated from peripheral leukocytes. In obese patients, plasma concentrations of leptin, lipids, glucose and insulin, the homeostasis model assessment index and blood pressure have been measured. The Trp64Arg mutation was identified with the real-time TaqMan method. Results: Neither genotype distribution nor allele frequency differed between the two groups. The metabolic syndrome prevalence was 59% in obese subjects, and was higher in men than in women (65 vs 55%: P ¼ 0.03). The body mass index (BMI) was related to age tertiles (b ¼ 0.08; Po0.001; multiple linear regression) in Trp64Arg-positive obese subjects. Conclusion: We confirm the high prevalence of the metabolic syndrome among severely obese subjects. ADRB3 polymorphism was significantly related to insulin resistance only in obese male subjects. Moreover, increased BMI was related to age in obese subjects with the ADRB3 polymorphism.

Molecular Analysis of the Adiponectin Gene in Severely Obese Patients from Southern Italy

“…In this study, severely obese patients have been examined for the presence of MS that represents an important summary of cardiovascular risk factors and the obese males show a higher, though statistically not significant, prevalence of MS; these results were in accordance with previous observations from our group [37,38] .…”

Candidate gene association study conditioning on individual ancestry in patients with type 2 diabetes and metabolic syndrome from Mexico City

“…It is interesting to note that the signal remains significant in the model controlling for BMI, indicating that the association of this polymorphism is not mediated exclusively through its effects on overall obesity. We also observed a significant association of ADRB3 (rs4994) and MS, indicating a potential role of this polymorphism in key components of the MS, such as insulin resistance, dyslipidemia or diastolic blood pressure, in accordance to what has been described in previous studies [53,57,58]. There is evidence indicating that the Arg variant alters beta-3 adrenoreceptor function by decreasing agonist sensitivity [59] but further research is necessary to decipher the mechanisms through which this missense polymorphism has an impact on metabolic traits.…”

Metabolic and Nutritional Status Changes After 10% WeightLoss in Severely Obese Patients Treated with Laparoscopic Surgery vs Integrated Medical Treatment

“…A similar figure for nonalcoholic fatty liver disease (NAFLD) has been reported [2,3]. Both reach a very high prevalence in severe obesity: about 60% in men and 55% in women for MS, as reported previously also by our group [4], and 90% in NAFLD [5], respectively.…”