Metabolism of asymmetric dimethylarginine and its clinical significance

Erbil, M.; Kurt, Yasemin Gülcan; Yaman, Halıl; Çakır, Erdinç; Akgül, Emin Özgür; Çaycı, Tuncer

doi:10.5505/tjb.2012.76486

Cited by 11 publications

(15 citation statements)

References 60 publications

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“…In contrast to ADMA levels, arginine and citrulline levels were sig- indicated that connection of protein degradation degree with the formation of ADMA is not known and ADMA is not the only factor in the pathogenesis of cardiovascular disease, probably the interactions of protein methylation, DDAH, ADMA and NO pathways are contributed to pathogenesis [11].…”

Section: Resultsmentioning

confidence: 96%

“…Increased levels of ADMA accompanied by cardiovascular risk factors such as hypertension, smoking, diabetes mellitus, dyslipidemia, insulin resistance and lead to vascular injury and increase the atherosclerosis have been reported [11,22]. BD is a systemic inflammatory vasculitis with unknown etiology.…”

Section: Resultsmentioning

confidence: 99%

“…ADMA generation occurs after the methylation of arginine residues in proteins. Proteolysis of methylated proteins to free amino acids generates free ADMA [11]. Increased plasma ADMA levels in vascular diseases are accepted as vascular risk factor [12].…”

Section: Introductionmentioning

confidence: 99%

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Asymmetric dimethylarginine levels in Behçet's Disease

Öztürk¹,

Vatansev²,

Taner³

et al. 2014

Turk J Bioch

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Objective: Behçet's disease (BD) is a chronic, inflammatory, multisystem vasculitis and the etiology is not yet fully understood. Nitric Oxide (NO) is an important molecule for the vascular system which synthesised by the Nitric Oxide Synthase (NOS) enzyme. Asymmetric dimethylarginine (ADMA) is an endogenous inhibitor of NOS. Therefore, we aimed to investigate levels of the ADMA and its relation with exacerbations in BD patients. Methods:The subjects enrolled in this study were recruited from 34 patients with BD and 34 healthy controls. Serum levels of ADMA, arginine and citrulline were measured by HPLC with fluorescence detection. The ESR and CRP levels analyzed by routinely used methods. Results: In contrast to information in the literature, ADMA levels were found to be decreased in BD patients when compared to control. Inversely, Arginine and Citrulline levels were significantly increased in BD patients. Increased Arginine/ADMA ratios were found in the patient group. There was an inverse relation between ADMA and CRP levels. Arginine values were also correlated CRP and Citrulline levels. Conclusion: One of the reasons of high number of BD attacks in young age may be low levels of ADMA. Our study suggests that the reduced levels of ADMA in patients group may impose a possible preventive role to ADMA through decrease of NO-mediated inflammation and exacerbations of BD with aging. and myocardial infarction; (c) diabetes mellitus, renal and hepatic diseases, active infectious diseases; (d) other known chronic inflammatory diseases. The body mass index (BMI) and blood pressure were recorded. It was also noted that whether the subjects smoke or not. Each subject was evaluated in active and inactive disease periods. At the time of examination, the presence of two or more of the following Behçet's clinical features was considered as active disease: Oral ulceration, genital ulceration, skin lesions, ocular lesions, active major vessel disease, and active major organ involvement including active gastrointestinal or neurological lesions. Subjects with one lesion or lesion-free period were regarded in inactive disease period.The protocol was approved by the Ethics Committee of the Selcuklu Medical Faculty (24.06.2010Faculty (24.06. -2010) and all subjects volunteered for the trial and written consent was obtained according to the Declaration of Helsinki.Blood samples were centrifuged with 2000 x g for 10 min at +4°C, followed by serum removal for assays. All serum samples were stored at -80°C until analysis.Measurements of ADMA, arginine and citrulline levels were accomplished by high performance liquid chromatography (HPLC), using the method described by Taner et al [17]. In brief, 20 mg 5-sulfosalisilic acid was added to 1 ml serum, and the mixture was left in an ice bath for 10 min. The precipitated protein was removed by centrifugation at 2,000×g for 10 min. Ten µl of the supernatant was filtered using a 0.45-μm pore size filter, which was then mixed with 100 μl derivatization reagent [prepared by dissolving ...

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Section: Resultsmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 99%

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Asymmetric dimethylarginine levels in Behçet's Disease

Öztürk¹,

Vatansev²,

Taner³

et al. 2014

Turk J Bioch

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“…There are some limitations to our study. In addition to the limited number of participants, several publications in the literature suggest that a few metabolic disorders, insulin resistance, glucose tolerance, diabetes mellitus, hyperlipidemia, chronic kidney disease, hypertension, and coronary heart diseases may be associated with increased ADMA levels (61,62). Moreover, in this study, we found that total cholesterol and triglyceride levels in OCD patients were higher compared to those in the control group, but there was no difference between the groups in terms of blood glucose, urea, and creatinine levels.…”

Section: Discussionmentioning

confidence: 99%

Levels of nitric oxide, asymmetric dimethyl arginine, symmetric dimethyl arginine,and L-arginine in patients with obsessive-compulsive disorder

Yilmaz¹,

Üstündağ²,

Gençer³

et al. 2016

Turk J Med Sci

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“…One of the main reasons and consequence of endothelial dysfunction is impaired nitric oxide (NO) bioavailability [6]. NO bioavailability is mostly regulated by the activity of nitric oxide synthase (NOS, EC.1.14.13.39).…”

Section: Introductionmentioning

confidence: 99%

Asymmetric dimethylarginine and arginine levels in patients with rheumatoid arthritis

Vatansev

Öztürk²,

Yılmaz

et al. 2013

Turk J Bioch

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Objective: Rheumatoid arthritis (RA) is a chronic inflammatory disease and it is associated with premature atherosclerosis development. RA patients have multiple factors to develop premature atherosclerosis. Enhanced asymmetric dimethylarginine (ADMA) is considered as a risk factor for the development of atherosclerosis by inducing endothelial dysfunction. The aim of this study was to investigate the role of asymmetric dimethylarginine, arginine and citrulline levels in patients with RA and their relation to the disease activity parameters for possible role on the disease activity. Methods: 92 patients with RA and 34 healthy controls were included the study. Patients and control blood samples collected for ADMA, arginine and citrulline levels. ADMA, arginine and citrulline levels were measured by pre-column derivatization fluorescence HPLC method. CRP and ESR levels examined on the same day were taken from the patient records. Results: While ADMA levels significantly increased, arginine levels decreased in the patient group. There was no difference in citrulline levels between both groups. Only citrulline and arginine showed a weak positive correlation. ADMA, arginine, and citrulline levels showed no correlation with either biochemical parameters such as CRP or ESR, or disease activity or disease severity. Conclusion: Our study has shown that an increase in ADMA levels with the decrease in arginine levels suggests an increase in arginine turnover to ADMA. Increased ADMA levels can be used for an increased risk for premature development of atherosclerosis in patients with RA. Since high ADMA levels prevent the NO-dependent vasodilatation, a possible role of ADMA in development of premature atherosclerosis may be independent from inflammation.

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Metabolism of asymmetric dimethylarginine and its clinical significance

Cited by 11 publications

References 60 publications

Asymmetric dimethylarginine levels in Behçet's Disease

Asymmetric dimethylarginine levels in Behçet's Disease

Levels of nitric oxide, asymmetric dimethyl arginine, symmetric dimethyl arginine,and L-arginine in patients with obsessive-compulsive disorder

Asymmetric dimethylarginine and arginine levels in patients with rheumatoid arthritis

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