Metabolism of carbamate insecticides by resting cells and cell-free preparations of a soil bacterium,Arthrobacter sp.

Sethunathan

J. of Env. Sc. & Hlth., Part B

1998

Self Cite

The role of microorganisms in the degradation of carbosulfan (2,3-dihydro-2,2-dimethyl-7-benzofuranyl-(di-n-butyl)-aminosulfenyl++ + methyl carbamate), an analogue of carbofuran (2,3-dihydro-2,2-dimethyl-7-benzofuranyl-N-methyl carbamate) was studied by selective enrichment of microorganisms degrading either or both insecticides by repeated application of the insecticides, individually or in combination to flooded soil. Soil suspension from the pots treated with carbosulfan and carbofuran, individually or in combination collected after two applications, effected distinctly more rapid hydrolysis of carbosulfan than did the suspension from untreated pots or the uninoculated medium. The rate of hydrolysis was further accelerated by soil suspensions collected after six applications of the insecticides in the order carbosulfan treated > carbofuran treated > carbosulfan + carbofuran treated. The ability of treated and untreated suspension to degrade carbofuran was also studied. The rate of degradation of carbofuran by the suspension was in the order carbofuran retreated > carbosulfan + carbofuran retreated > carbosulfan retreated soil. Further involvement of microorganism in the rapid degradation of carbosulfan was confirmed by testing the degradation in sterilized and nonsterilized enrichment culture.

Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 98%

Microbial degradation of carbosulfan by carbosulfan - and carbofuran - retreated rice soil suspension

Sethunathan

J. of Env. Sc. & Hlth., Part B

1998

Self Cite

World J Microbiol Biotechnol

“…Accumulation of carbofuran and its metabolites in the environment could potentially pose health hazards (Chaudhry et al 2002a). There are several reports on the biodegradation of carbofuran, carbofuran phenol and its metabolites by free microbial cells including strains such as Achromonobacter, Flavobacterium, Sphingomonas, Arthrobacter, Pseudomonas (Chaudhry and Ali 1988;Tomasek and Karns 1989;Chapalmandugu and Chaudhry 1992;Feng et al 1997;Ramanand et al 1991;Desaint et al 2000;Chaudhry et al 2002b), Mucor ramanians and Actinomycetes (Jiyoung et al 2007). However the biodegradation of carbofuran phenol by immobilized cells of microorganisms has not yet been investigated.…”

Section: Introductionmentioning

confidence: 96%

Biodegradation of Carbofuran phenol by free and immobilized cells of Klebsiella pneumoniae ATCC13883T

Kadakol

Kamanavalli

Shouche

2010

The Klebsiella sp. strain ATCC13883T capable of degrading carbofuran phenol (2,3-dihydro-2,2-dimethylbenzofuran-7-ol) has been separated from the soil by enrichment culture technique and immobilized in various, namely polyurethane foam (PUF), polyacrylamide, alginate, agar and alginate-bentonite clay-powdered activated charcoal (PAC). The degradation rates of 20 and 30 mM carbofuran phenol by free and immobilized cells in batch and semi-continuous shaken cultures were compared. The PUF-immobilized cells achieved higher degradation rates in a shorter time than freely suspended cells and the cells immobilized in polyacrylamide, alginate and agar. The PUF-and alginate-bentonite clay-PAC-immobilized cells could be reused for more than 36 cycles, polyacrylamideentrapped cells for 20 cycles and alginate-bentonite-PAC 28 cycles, without losing any degradation capacity and showed better tolerance to pH, temperature and concentration changes than free cells. These results showed that cells immobilized in modified alginate-bentonite-PAC immobilizers tolerated and completely degraded carbofuran phenol at initial concentrations of 20 and 30 mM and also higher. Such a bacterial strain could be used for bioremediation of environments contaminated with phenolic compounds.

“…CF06, Pseudomonas sp. 50432, Pseudomonas putida, Sphingomonas paucimobilis, and Streptomyces violaceusniger [9,[11][12][13][14][15][16]. However, no studies have isolated carbofuran-degrading bacteria from river biofilms in agricultural areas.…”

Section: Introductionmentioning

confidence: 99%

Accessing the Carbofuran Degradation Ability of Cultures From Natural River Biofilms in Different Environments

Tien

Huang

Chen

2017

CLEAN Soil Air Water

Acclimatized microbial consortia and bacterial strains were isolated from natural river biofilms to investigate their carbofuran degradation ability. Under 28‐day continuous enrichment culturing of river biofilms, the acclimatized microbial consortia were obtained and showed a high carbofuran removal rate (52.5%). The batch experiments showed that 28‐ and 35‐day loofah‐sponge‐attached acclimatized consortia (i.e., microbial consortia A and B) had low carbofuran removal rates. However, after preservation, the carbofuran degradation abilities of both microbial consortia recovered possibly due to the growth of carbofuran degraders. Denaturing gradient gel electrophoresis (DGGE) analysis revealed that Stenotrophomonas sp., Pseudomonas fulva, Comamonas jiangduensis, and Thermolithobacter sp. dominated in both microbial consortia. The best preservation conditions to have high carbofuran removal for microbial consortia A and B were −20°C for 3 months (55.7% removal) and 25°C for 1 month (73.9% removal), respectively. The isolated Sphingobacterium multivorum showed high carbofuran removal rates with and without sugar (67.4 and 73.1% removal) at pH 7 (25°C), followed by those at pH 7 (30°C) and pH 9 (25°C) (41.2 and 32.1% removal). This strain was able to degrade carbofuran in the simulated carbofuran‐contaminated paddy fields. The study demonstrated that the immobilized microbial consortia and S. multivorum from river biofilms are good biomaterials for bioremediating carbofuran‐contaminated sites.