Metabolism of Different PCB Congeners in Plant Cell Cultures

Wilken, Annelen; Bock, Claudia; Bokern, Maria; Harms, H.

doi:10.1897/1552-8618(1995)14[2017:modpci]2.0.co;2

Cited by 24 publications

(26 citation statements)

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“…The OH-PBDE congeners 3′-OH-2,4-BDE-7, 2′-OH-4-BDE-7, 3′-OH-BDE-17, 3-OH-BDE-47, and 5-OH-BDE-47 were found in plant tissues (Tables S4 and S5) (15) and sewage effluents (16) and marine organisms (18), as well as human blood (19). No report is available on OH-PBDE analysis in plant tissues, but previous studies on metabolism of PCBs in plants have demonstrated that lower chlorinated PCBs are degraded more readily than higher chlorinated ones (27).…”

Section: Resultsmentioning

confidence: 99%

Behavior of Decabromodiphenyl Ether (BDE-209) in the Soil−Plant System: Uptake, Translocation, and Metabolism in Plants and Dissipation in Soil

Huang

Zhang

Christie

et al. 2009

Environ. Sci. Technol.

193

138

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Deca-bromodiphenyl ether (BDE-209) is the major component of the commercial deca-BDE flame retardant. There is increasing concern over BDE-209 due to its increasing occurrence in the environment and in humans. In this study the behavior of BDE-209 in the soil-plant system was investigated. Accumulation of BDE-209 was observed in the roots and shoots of all the six plant species examined, namely ryegrass, alfalfa, pumpkin, summer squash, maize, and radish. Root uptake of BDE-209 was positively correlated with root lipid content (P < 0.001, R 2 ) 0.81). The translocation factor (TF, C shoot /C root ) of BDE-209 was inversely related to its concentration in roots. Nineteen lower brominated (di-to nona-) PBDEs were detected in the soil and plant samples and five hydroxylated congeners were detected in the plant samples, indicating debromination and hydroxylation of BDE-209 in the soil-plant system. Evidence of a relatively higher proportion of penta-through di-BDE congeners in plant tissues than in the soil indicates that there is further debromination of PBDEs within plants or low brominated PBDEs are more readily taken up by plants. A significant negative correlation between the residual BDE-209 concentration in soil and the soil microbial biomass measured as the total phospholipid fatty acids (PLFAs) (P < 0.05, R 2 ) 0.74) suggests that microbial metabolism and degradation contribute to BDE-209 dissipation in soil. These results provide important information about the behavior of BDE-209 in the soilplant system.

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Section: Resultsmentioning

confidence: 99%

Behavior of Decabromodiphenyl Ether (BDE-209) in the Soil−Plant System: Uptake, Translocation, and Metabolism in Plants and Dissipation in Soil

Huang

Zhang

Christie

et al. 2009

Environ. Sci. Technol.

193

138

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“…According to our results, nettles are only able to remediate less chlorinated biphenyls. This is not very surprising, because it was previously shown that the lower chlorination grade is associated with higher metabolism rates [28]. A decrease of up to 33% was determined for trichlorinated biphenyls (congeners 13–39).…”

Section: Resultsmentioning

confidence: 74%

Native Phytoremediation Potential of Urtica dioica for Removal of PCBs and Heavy Metals Can Be Improved by Genetic Manipulations Using Constitutive CaMV 35S Promoter

et al. 2016

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Although stinging nettle (Urtica dioica) has been shown to reduce HM (heavy metal) content in soil, its wider phytoremediation potential has been neglected. Urtica dioica was cultivated in soils contaminated with HMs or polychlorinated biphenyls (PCBs). After four months, up to 33% of the less chlorinated biphenyls and 8% of HMs (Zn, Pb, Cd) had been removed. Bacteria were isolated from the plant tissue, with the endophytic bacteria Bacillus shackletonii and Streptomyces badius shown to have the most significant effect. These bacteria demonstrated not only benefits for plant growth, but also extreme tolerance to As, Zn and Pb. Despite these results, the native phytoremediation potential of nettles could be improved by biotechnologies. Transient expression was used to investigate the functionality of the most common constitutive promoter, CaMV 35S in Urtica dioica. This showed the expression of the CUP and bphC transgenes. Collectively, our findings suggest that remediation by stinging nettle could have a much wider range of applications than previously thought.

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“…An apparent K m value of 8.6 m was determined with microsomes of U. lactuca. Upon GC-MS, the product gave a prominent mass fragment at m/z 205 and a chlorine side-peak at m/z 207, as is typical for monohydroxymonochlorobiphenyls (Wilken et al, 1995).…”

Section: Enzyme Distributionsmentioning

confidence: 89%

Cytochrome P450 Monooxygenases for Fatty Acids and Xenobiotics in Marine Macroalgae1

Pflugmacher

Sandermann

1998

Plant Physiology

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The metabolism of xenobiotics has mainly been investigated in higher plant species. We studied them in various marine macroalgae of the phyla Chlorophyta, Chromophyta, and Rhodophyta. Microsomes contained high oxidative activities for known cytochrome (Cyt) P450 substrates (fatty acids, cinnamic acid, 3-and 4-chlorobiphenyl, 2,3-dichlorobiphenyl, and isoproturon; up to 54 pkat/mg protein). The presence of Cyt P450 (approximately 50 pmol/mg protein) in microsomes of the three algal families was demonstrated by CO-difference absorption spectra. Intact algal tissue converted 3-chlorobiphenyl to the same monohydroxymetabolite formed in vitro. This conversion was 5-fold stimulated upon addition of phenobarbital, and was abolished by the known P450 inhibitor, 1-aminobenzotriazole. It is concluded that marine macroalgae contain active species of Cyt P450 and could act as a metabolic sink for marine pollutants.

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Metabolism of Different PCB Congeners in Plant Cell Cultures

Cited by 24 publications

References 0 publications

Behavior of Decabromodiphenyl Ether (BDE-209) in the Soil−Plant System: Uptake, Translocation, and Metabolism in Plants and Dissipation in Soil

Behavior of Decabromodiphenyl Ether (BDE-209) in the Soil−Plant System: Uptake, Translocation, and Metabolism in Plants and Dissipation in Soil

Native Phytoremediation Potential of Urtica dioica for Removal of PCBs and Heavy Metals Can Be Improved by Genetic Manipulations Using Constitutive CaMV 35S Promoter

Cytochrome P450 Monooxygenases for Fatty Acids and Xenobiotics in Marine Macroalgae1

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