Metabolites from symbiotic bacteria of entomopathogenic nematodes have antimicrobial effects against Pythium myriotylum

Shan, Shaojie; Ma, Hui; Li, Yang; Huang, Cuiqing; Gu, Xinghui; Jiang, Zhensheng; Sun, Bingjiao; Chen, Chaoying; Wei, Xianqin; Shen, Guangshuang; Shapiro‐Ilan, David I.; Ruan, Weibin

doi:10.1007/s10658-020-02053-2

Cited by 9 publications

(4 citation statements)

References 41 publications

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“…for both F. carpophilum and F. effusum, except for metabolites from Xenorhabdus nematophila, which displayed intermediate results when tested on F. carpophilum spores. In contrast, the inhibitory effect of Xenorhabdus and Photorhabdus metabolites seemed to depend on the isolate tested, not on the genus when tested against Pythium myriotylum spores [51]. Spore germination is a critical step in the development of FHB disease in wheat seedlings.…”

Section: Discussionmentioning

confidence: 86%

“…Shan et al [51] mentioned that the antimicrobial activity of symbiotic bacteria derived from EPNs against the mycelial growth of plant pathogens depends on the species of symbiotic bacteria. Photorhabdus luminescens SGI 170 followed X. khoisanae SGI 197 as the second highest performing isolate, with a 95.79% mean inhibition rate after 7 days' incubation.…”

Section: Discussionmentioning

confidence: 99%

“…are known as key producers of trans-cinnamic acid (TCA) [50]. Trans-cinnamic acid is said to have antifungal properties [39,50,51]. Lalramchuani et al [56] reported raising the inhibition rate using a Photorhabdus sp.…”

Section: Discussionmentioning

confidence: 99%

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Antifungal Effect of Metabolites from Bacterial Symbionts of Entomopathogenic Nematodes on Fusarium Head Blight of Wheat

Kgosiemang,

Ramakuwela,

Figlan

et al. 2024

JoF

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Fungal diseases such as Fusarium head blight (FHB) are significant biotic stressors, negatively affecting wheat production and quality. This study explored the antifungal activity of the metabolites produced by the bacterial symbionts of entomopathogenic nematodes (EPNs) against FHB-causing Fusarium sp. Fusarium graminearum. To achieve this, the symbiotic bacteria of nine EPN isolates from the EPN collection at the Agricultural Research Council-Small Grains (ARC-SG) were isolated from the cadavers of Galleria mellonella (Lepidoptera: Pyralidae) larvae after infection with EPNs. Broth cultures (crude) and their supernatants (filtered and autoclaved) of each bacterial isolate were used as bacterial metabolite treatments to test their inhibitory effect on the mycelial growth and spore germination of F. graminearum. Mycelial growth inhibition rates varied among both bacterial isolates and treatments. Crude metabolite treatments proved to be more effective than filtered and autoclaved metabolite treatments, with an overall inhibition rate of 75.25% compared to 23.93% and 13.32%, respectively. From the crude metabolite treatments, the Xenorhabdus khoisanae SGI 197 bacterial isolate from Steinernema beitlechemi SGI 197 had the highest mean inhibition rate of 96.25%, followed by Photorhabdus luminescens SGI 170 bacteria isolated from Heterorhabditis bacteriophora SGI 170 with a 95.79% mean inhibition rate. The filtered metabolite treatments of all bacterial isolates were tested for their inhibitory activity against Fusarium graminearum spore germination. Mean spore germination inhibition rates from Xenorhabdus spp. bacterial isolates were higher (83.91 to 96.29%) than those from Photorhabdus spp. (6.05 to 14.74%). The results obtained from this study suggest that EPN symbiotic bacterial metabolites have potential use as biological control agents of FHB. Although field efficacy against FHB was not studied, the significant inhibition of mycelial growth and spore germination suggest that the application of these metabolites at the flowering stage may provide protection to plants against infection with or spread of F. graminearum. These metabolites have the potential to be employed as part of integrated pest management (IPM) to inhibit/delay conidia germination until the anthesis (flowering stage) of wheat seedlings has passed.

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Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

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Antifungal Effect of Metabolites from Bacterial Symbionts of Entomopathogenic Nematodes on Fusarium Head Blight of Wheat

Kgosiemang,

Ramakuwela,

Figlan

et al. 2024

JoF

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“…akan berubah warna menjadi merah kehitaman, tekstur tubuh menjadi lunak dan jaringan hancur. [11]. Entomotoksin yang dihasilkan Photorhabdus spp.…”

Section: Tahapan Penelitianunclassified

Patogenitas Nematoda Entomopatogen Heterorhabditis spp. Terhadap Larva Spodoptera litura

Maulida

Rokhim

Zahro’in³

2021

SST

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Spodoptera litura is a pest in vegetable plants that attacks the leaves and stems. Damage arising from the attack of S. litura can decrease the productivity of plants. Synthetic pesticides are often used in controlling the population of S. litura, but synthetic pesticides have a high negative impact. Potential entomopathogenic nematode Heterorhabditis spp. believed to be effective for controlling pest populations. This study aims to find out the pathogenic value of entomopathogenic nematode Heterorhabditis spp. to the larvae of S. litura. Based on the results obtained Heterorhabditis spp. has a positive influence in causing mortality in the larvae of S. litura. Heterorhabditis spp. mortality of up to 42%. Symptoms indicated by larvae of S. litura affected by Heterorhabditis spp. among them the behavior becomes passive, the body becomes flaccid, the cuticle turns red and the tissues inside the body are destroyed. Obtained pathogenicity value Lc 50 Heterorhabditis spp. 7,690 IJ/ml, as for the factors affecting Heterorhabditis spp. such as humidity, temperature, pH, and light intensity.Keywords – Entomopathogenic Nematodes, Pathogenicity, Spodoptera litura

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Potential of entomopathogenic nematode‐infected insect cadavers for the biocontrol of the red imported fire ant Solenopsis invicta

Tang

Zhang

et al. 2023

Pest Management Science

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BackgroundEntomopathogenic nematodes (EPNs) in an aqueous suspension treatment showed potential in the biocontrol of the red imported fire ant Solenopsis invicta, whereas colony relocation following this inundative application had restricted overall effectiveness. As a novel strategy, EPN pre‐infected insect cadavers carrying the next generation of nematode‐infective juveniles (IJs) might be used to efficiently manage insect pests. However, this strategy has not been tested on S. invicta. This study evaluated EPNs emerging from wax moth Galleria mellonella cadavers to facilitate infection of S. invicta compared to those used in aqueous suspensions.ResultsAmong seven EPN species, Steinernema riobrave and Heterorhabditis bacteriophora had the highest insecticidal efficiency in aqueous treatment. Cadavers of G. mellonella colonized by either one of two EPN species were not damaged by worker ants, ensuring that the IJs could develop within and emerge successfully from the cadavers. Likewise, compared to an aqueous suspension treatment with an equivalent number of IJs, treatment with an S. riobrave‐infected cadaver increased the mortality of S. invicta by ≈10%, whereas infection by H. bacteriophora did not differ between treatments. However, the coexistence of S. riobrave‐ and H. bacteriophora‐infected cadavers adversely affected the control of S. invicta, likely as a result of competition resulting from the increased dispersal of each emerging EPN species.ConclusionUsing EPN‐infected G. mellonella cadavers as a strategy increased the mortality of S. invicta in the laboratory. This study provides positive evidence for the future applications of S. riobrave‐infected cadavers in the biocontrol of red imported fire ants. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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Metabolites from symbiotic bacteria of entomopathogenic nematodes have antimicrobial effects against Pythium myriotylum

Cited by 9 publications

References 41 publications

Antifungal Effect of Metabolites from Bacterial Symbionts of Entomopathogenic Nematodes on Fusarium Head Blight of Wheat

Antifungal Effect of Metabolites from Bacterial Symbionts of Entomopathogenic Nematodes on Fusarium Head Blight of Wheat

Patogenitas Nematoda Entomopatogen Heterorhabditis spp. Terhadap Larva Spodoptera litura

Potential of entomopathogenic nematode‐infected insect cadavers for the biocontrol of the red imported fire ant Solenopsis invicta

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