Abstrak Softgellcandy adalah permen bertekstur lunak yang diproses dengan penambahan komponen hidrokoloid seperti agar, gum, pektin, pati, kaegenan, gelatin dan lain-lain yang digunakan untuk modifikasi tekstur sehingga menghasilkan produk yang kenyal. Sayangnya distribusi Softgellcandy di pasaran seringkali terlepas dari pengawasan lembaga berwenang. Banyak ditemukan bermacam merk Softgellcandy yang tidak berBPOM maupun tidak berlabel Halal. Gelatin menjadi titik kritis kehalalan Softgellcandy. Penelitian ini menguji 15 sampel Softgellcandy tak berlabel halal yang dijual bebas di Surabaya dengan primer pengkode fragmen DNA cytochrome b Babi. Metode yang digunakan adalah konvensional PCR pada suhu 98oC-2 menit; 95oC-30 detik; 61oC-30 detik; 72oC-40 detik; dan 72oC-3 menit, selama 30 siklus. Visualisasi hasil PCR menggunakan elektroforesis 2% gel agarosa menunjukkan dari 15 sampel, 8 sampel terindikasi kontaminan DNA babi ditandai dengan pita DNA sebesar ±149bp. Pemerintah perlu melakukan monitoring lebih ketat terkait peredaran produk makanan tak berlabel halal yang dijual bebas di pasaran, mengingat Halal menjadi issue yang sangat sensitif di negara dengan mayoritas penduduk muslim terbesar di dunia ini. Karena halal adalah suatu keharusan. Keywords: Softgellcandy, cyt b Babi, PCR

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Halal food means food that permitted under Islamic law and fulfills about requirements. The absence of information about halal food contained in UIN Sunan Ampel Surabaya (UINSA) campus area causes related research to be carried out. This study aims to determine the porcine DNA contamination on food around UINSA area using molecular technology. Twenty two samples used were foods that contain meat and may contain pork obtained from canteens around UINSA area, analyzed using Polymerase Chain Reaction (PCR) method. The analysis was started with DNA isolation of 22 food samples, electrophoresis, PCR, then visualization gel electrophoresis. Primer gene coding for cytochrome b (cyt b) which produces 149 bp of DNA fragments. The results showed that no porcine contamination in 22 food samples, while the positive control showed a band of 149 bp. End point PCR method potentially to detect porcine DNA contaminants in food products around UINSA. Therefore the food is halal and safe for consumption.

Meat mills in Indonesia are not halal certified. Small traders who do not have a food processor facility will choose the public market. This can be done both by traders who sell halal processed food and processed pork. It is necessary to study the content of meat mills in the public market so that it becomes critical evidence of an institution authorized to issue a policy immediately. This study aims to examine the content of porcine DNA in meat mills in Pasar Surya Kota Surabaya using the Real Time PCR (qPCR) method. QPCR amplification was performed using a porcine probe primer with annealing temperature optimization of 60 o C. The amplification curve shows an increase in the curve in the positive control (EPC) with a value of Cq 34.00, while the negative control (NTC) is not amplified. The results of this study show that 2 out of 10 samples experienced an increase in the amplification curve on the FAM indicator, meaning that in the meat grinding sample there was a content of porcine DNA. The two samples are 8 and 9 with Cq values of 38.02 and 39.04, respectively. Market Halal Certification must be done immediately.

<p><strong>Spodoptera litura is a pest in vegetable plants that attacks the leaves and stems. Damage arising from the attack of S. litura can decrease the productivity of plants. Synthetic pesticides are often used in controlling the population of S. litura, but synthetic pesticides have a high negative impact. Potential entomopathogenic nematode Heterorhabditis spp. believed to be effective for controlling pest populations. This study aims to find out the pathogenic value of entomopathogenic nematode Heterorhabditis spp. to the larvae of S. litura. Based on the results obtained Heterorhabditis spp. has a positive influence in causing mortality in the larvae of S. litura. Heterorhabditis spp. mortality of up to 42%. Symptoms indicated by larvae of S. litura affected by Heterorhabditis spp. among them the behavior becomes passive, the body becomes flaccid, the cuticle turns red and the tissues inside the body are destroyed. Obtained pathogenicity value Lc 50 Heterorhabditis spp. 7,690 IJ/ml, as for the factors affecting Heterorhabditis spp. such as humidity, temperature, pH, and light intensity.</strong></p><p><em><strong>Keywords – </strong>Entomopathogenic Nematodes, Pathogenicity, Spodoptera litura</em></p>