Metabolomic analysis of rat plasma following chronic low-dose exposure to dichlorvos

Yang, Jiaying; Wang, H; Wang, Xu; Ding, Hao; Du, Longfei; Zhao, Xiujuan; Sun, Chongde

doi:10.1177/0960327112459533

Cited by 19 publications

(12 citation statements)

References 38 publications

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“…Metabonomics as a new technical platform has been widely used in toxicology. [21][22][23] In our study, PCA score plot and PLS-DA score plot showed clear separation between ASB-treated and control groups, which indicated that urine metabolic pattern significantly changed after the treatment of ASB.…”

Section: Discussionsupporting

confidence: 57%

Metabolic changes in the urine of andrographolide sodium bisulfite-treated rats

Xing

Yuan

et al. 2015

Hum Exp Toxicol

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In recent years, andrographolide sodium bisulfite (ASB) has been reported to cause acute renal failure frequently in clinical practice. We hypothesized that changes in metabolic profile could have occurred after administration of ASB. To investigate the metabolic changes caused by ASB-induced nephrotoxicity, metabonomics method was utilized to depict the urine metabolic characteristics and find the specific urine biomarkers associated with ASB-induced nephrotoxicity. Sprague-Dawley rats were randomly assigned into three experimental groups. They received a single daily injection of vehicle (0.9% sodium chloride solution) or ASB at a dose of 100 or 600 mg kg−1 day−1 for 7 days. Twelve-hour urine was collected after the last administration. The routine urinalysis was measured by a urine automatic analyzer while urinary metabolites were evaluated using gas chromatography/mass spectrometry. The acquired data were processed by multivariate principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA), and orthogonal PLS-DA. After 7-day administration of ASB, the positive urine samples in protein, occult blood, and ketones were increased, presenting dose dependence. The PCA and PLS-DA models were capable of distinguishing the difference between ASB-treated group and control. Biomarkers such as 1,5-anhydroglucitol, d-erythro-sphingosine, and 2-ketoadipate were identified as the most influential factors in ASB-induced nephrotoxicity.

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Section: Discussionsupporting

confidence: 57%

Metabolic changes in the urine of andrographolide sodium bisulfite-treated rats

Xing

Yuan

et al. 2015

Hum Exp Toxicol

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“…Our findings may also be in line with previous observations of higher plasma levels of type II PLA2 in sepsis patients, which significantly correlated with TNF-α, IL-6 and IL-8 levels [ 47 ]. On the other hand, elevated plasma levels of LysoPE (16:0/0:0) have been observed in rats with induced-liver injuries as a result of massive destruction of cell membranes [ 48 , 49 ]. Therefore, it is also possible that the high LysoPE levels may be the consequence of severe organ damage during systemic melioidosis.…”

Section: Discussionmentioning

confidence: 99%

Metabolomic Profiling of Plasma from Melioidosis Patients Using UHPLC-QTOF MS Reveals Novel Biomarkers for Diagnosis

Lau

Lee

et al. 2016

IJMS

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To identify potential biomarkers for improving diagnosis of melioidosis, we compared plasma metabolome profiles of melioidosis patients compared to patients with other bacteremia and controls without active infection, using ultra-high-performance liquid chromatography-electrospray ionization-quadruple time-of-flight mass spectrometry. Principal component analysis (PCA) showed that the metabolomic profiles of melioidosis patients are distinguishable from bacteremia patients and controls. Using multivariate and univariate analysis, 12 significant metabolites from four lipid classes, acylcarnitine (n = 6), lysophosphatidylethanolamine (LysoPE) (n = 3), sphingomyelins (SM) (n = 2) and phosphatidylcholine (PC) (n = 1), with significantly higher levels in melioidosis patients than bacteremia patients and controls, were identified. Ten of the 12 metabolites showed area-under-receiver operating characteristic curve (AUC) >0.80 when compared both between melioidosis and bacteremia patients, and between melioidosis patients and controls. SM(d18:2/16:0) possessed the largest AUC when compared, both between melioidosis and bacteremia patients (AUC 0.998, sensitivity 100% and specificity 91.7%), and between melioidosis patients and controls (AUC 1.000, sensitivity 96.7% and specificity 100%). Our results indicate that metabolome profiling might serve as a promising approach for diagnosis of melioidosis using patient plasma, with SM(d18:2/16:0) representing a potential biomarker. Since the 12 metabolites were related to various pathways for energy and lipid metabolism, further studies may reveal their possible role in the pathogenesis and host response in melioidosis.

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“…Therefore, metabonomics has been widely applied in many research areas, including the chemical safety of food, drug toxicology, biomarker discovery, functional genomics, and molecular pathology. 20,21 In our previous study, Yang et al 22 analyzed the toxic effects of exposure to low-level DDVP on rat plasma using ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). The results showed that lysoPC (15:0/0:0), lysoPC (16:0/0:0), lysoPC (17:0/0:0), lysoPC (0:0/18:0), sphingosine, sphinganine, C16 sphinganine, C17 sphinganine, and arachidonic acid (AA) significantly decreased while lysoPE (16:0/0:0) significantly increased in the DDVP-treated group compared with the control group.…”

Section: Introductionmentioning

confidence: 99%

A metabonomic analysis of the effect of quercetin on toxicity induced by chronic exposure to low-level dichlorvos in rat plasma

Zeng

Qı

et al. 2014

Mol. BioSyst.

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A previous study of ours has reported that chronic exposure to low-level dichlorvos (DDVP, 7.2 mg per kg bw) damages the liver, interferes with fatty acid metabolism, and disturbs the antioxidant defense system in rats. This study aims to investigate whether or not quercetin can protect against DDVP-induced toxicity through metabonomics and to elucidate the mechanism underlying this protective effect. Rats were randomly assigned into the control group, DDVP-treated group, quercetin-treated group, and quercetin plus DDVP-treated group. DDVP and quercetin were administered to the rats daily via drinking water and gavage, respectively, continuously for 90 d. The metabonomic profiles of rat plasma were analyzed using ultra-performance liquid chromatography-mass spectrometry. Finally, 11 metabolites were identified, including those of quercetin, isorhamnetin, and quercetin-3-glucuronide. The 11 metabolites showed significant changes in some treatment groups compared with the control group. Arachidonic acid, phytosphingosine, and C16 sphinganine significantly decreased while p-cresol, lysoPE (16:0/0:0), lysoPC (15:0/0:0), lysoPC (16:0/0:0), lysoPC (0:0/18:0), and tryptophan significantly increased in the DDVP-treated group compared with the control group. The tendency of the aforementioned metabolites to change was significantly ameliorated in the high-dose quercetin (50 mg per kg bw per day) plus DDVP-treated group compared with the DDVP-treated group. However, the levels of these metabolites in the high-dose quercetin plus DDVP-treated group were still significantly different from those in the control group. The results indicate that high-dose quercetin (50 mg per kg bw per day) elicits a partial protective effect on DDVP-induced toxicity. The histopathology of the liver tissues was consistent with the above results. Quercetin demonstrated regulatory effects on the metabolism of lipids and amino acids, the antioxidant defense system, etc. Therefore, increasing the daily intake of quercetin can ameliorate the toxicity induced by chronic exposure to low-level DDVP residue in food and/or water.

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Metabolomic analysis of rat plasma following chronic low-dose exposure to dichlorvos

Cited by 19 publications

References 38 publications

Metabolic changes in the urine of andrographolide sodium bisulfite-treated rats

Metabolic changes in the urine of andrographolide sodium bisulfite-treated rats

Metabolomic Profiling of Plasma from Melioidosis Patients Using UHPLC-QTOF MS Reveals Novel Biomarkers for Diagnosis

A metabonomic analysis of the effect of quercetin on toxicity induced by chronic exposure to low-level dichlorvos in rat plasma

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