Metabolomic analysis of the effects of omeprazole and famotidine on aspirin-induced gastric injury

Takeuchi, Kenichiro; Ohishi, Maki; Endo, Keiko; Suzumura, Ken-ichi; Naraoka, Hitoshi; Ohata, Takeji; Seki, Jiro; Miyamae, Yoichi; Honma, Masashi; Soga, Tomoyoshi

doi:10.1007/s11306-014-0627-0

Cited by 13 publications

(14 citation statements)

References 29 publications

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“…Because this compound is one of the intermediates in the TCA cycle, this alteration might suggest the disturbance of energy metabolism in GU [31]. In a previous study on gastric ulcer, alteration of cis-aconitate was observed which confirms this result [32]. Glycine was decreased in treated group tissues which could be due to elevated energy consuming in order to protect against gastric damage.…”

Section: Discussionsupporting

confidence: 62%

Therapeutic effects of hydro-alcoholic extract of Achillea wilhelmsii C. Koch on indomethacin-induced gastric ulcer in rats: a proteomic and metabolomic approach

Koushki

Yekta

Amiri-Dashatan

et al. 2019

BMC Complement Altern Med

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Background: Gastric ulcer is one of the most prevalent diseases worldwide. In Iranian folk medicine, Achillea wilhelmsii (AW) is used as a treatment for gastric ulcer. Previous reports also mentioned Antiulcerogenic properties for this herbal plant. This study investigated the therapeutic effects of Achillea wilhelmsii C. Koch extract on indomethacin-induced gastric lesion in rats, from both proteomic and metabolomic perspectives. Methods: The rats were divided into 4 groups. Gastric ulceration was induced by a single dose of indomethacin (45 mg/kg) by oral gavage. An amount of 800 mg/kg of AW extract was administered orally. Serum and tissue samples were collected for further investigations. The metabolomic study was performed by 1 H-NMR CPMG spectrometry. Proteomic analysis was also executed by using two dimensional gel electrophoresis (2DE) followed by liquid chromatography coupled to tandem mass spectrometry (LC-ESI/MS/MS). Real time PCR was used to confirm some of the genes. Results: The macroscopic and microscopic investigations confirmed the effectiveness of the AW extract. There was a panel of metabolites which showed alteration during gastric lesion development. The levels of some of these metabolite reversed nearly to their control values after the administration of AW extract. There were also changes in the levels of some proteins including Alb, Fabp5, Hspb1, Tagln, Lgals7, Csta and Myl9 which were reversed after AW administration. Conclusions: Our findings suggested that Achillea wilhelmsii C. Koch extract could be a potential therapy to be used for indomethacin-induced gastric lesion treatment in the future. However, further investigations are needed to confirm the results.

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Section: Discussionsupporting

confidence: 62%

Therapeutic effects of hydro-alcoholic extract of Achillea wilhelmsii C. Koch on indomethacin-induced gastric ulcer in rats: a proteomic and metabolomic approach

Koushki

Yekta

Amiri-Dashatan

et al. 2019

BMC Complement Altern Med

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“…Following centrifugal filtration through a Millipore 5-kDa cutoff filter, the filtrate was lyophilized and dissolved in 50 μL of Milli-Q water containing reference compounds (200 μM 3-aminopyrrolidine and 200 μM trimesate). Metabolite standards for identification of metabolites and CE-TOF–MS conditions for metabolite analysis were as used in our previous study 6. All CE-TOF–MS experiments were performed using an Agilent 1600 CE capillary electrophoresis system (Agilent Technologies, Waldbronn, Germany), Agilent G3250AA LC/MSD TOF–MS system (Agilent Technologies, Palo Alto, CA, USA), Agilent 1100 isocratic HPLC pump, Agilent G1603A CE–MS adapter kit, and Agilent G1607A CE-electro spray ionization (ESI)–MS sprayer kit.…”

Section: Methodsmentioning

confidence: 99%

“…System control and data acquisition were done using the Agilent G2201AA ChemStation software for CE and Analyst QS software for TOF–MS. Annotation tables were generated by the same method used in our previous study 5,6…”

Section: Methodsmentioning

confidence: 99%

Hydroxyproline, a Serum Biomarker Candidate for Gastric Ulcer in Rats: A Comparison Study of Metabolic Analysis of Gastric Ulcer Models Induced by Ethanol, Stress, and Aspirin

et al. 2014

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Gastrointestinal symptoms are a common manifestation of adverse drug effects. Non-steroid anti-inflammatory drugs (NSAIDs) are widely prescribed drugs that induce the serious side effect of gastric mucosal ulceration. Biomarkers for these side effects have not been identified and ulcers are now only detectable by endoscopy. We previously identified five metabolites as biomarker candidates for NSAID-induced gastric ulcer using capillary electrophoresis–mass spectrometry (CE–MS)-based metabolomic analysis of serum and stomach from rats. Here, to clarify mechanism of changes and limitations of indications of biomarker candidates, we performed CE–MS-based metabolomic profiling in stomach and serum from rats with gastric ulcers induced by ethanol, stress, and aspirin. The results suggest that a decrease in hydroxyproline reflects the induction of gastric injury and may be useful in identifying gastric ulcer induced by multiple causes. While extrapolation to humans requires further study, hydroxyproline can be a new serum biomarker of gastric injury regardless of cause.

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“…QQQ mass accuracy L3 6 groups: water-occasion A Ao (n = 6), water and paracetamol-occasion A A1 (n = 6), water-occasion B Bo (n = 6), water and paracetamol-occasion B B1 (n = 6), water-occasion C Co (n = 6) and water and paracetamoloccasion C C1 (n = 6) future science group Review García, Godzien, Lopez-Gonzalves & Barbas profiles in plasma showed better stability than those in serum. Soga's group optimized a strategy to purify and extract metabolites from plasma or serum samples based on plasma:methanol:water:chloroform in a (1:9:2:10) ratio, followed by centrifugal filtration through a 5-kDa cut-off, evaporation and resuspension in the same volume [38,[44][45][46][47][48][49][50] or half volume [51] of purified water. Since 2006 [52] they are using this protocol for their global approach with CE-MS as a routine method.…”

Section: Plasma and Serummentioning

confidence: 99%

“…Figure 1, to study metabolites in different biological samples with special protocols focused on extraction of polar and ionizable compounds. As the migration time reproducibility is lower with CE-MS than with other MS-chromatographic couplings, the use of one or several internal standards is a common approach [33][34][35][36][37][38].…”

mentioning

confidence: 99%

Capillary Electrophoresis Mass Spectrometry as A Tool for Untargeted Metabolomics

et al. 2016

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Highly polar and ionic metabolites, such as sugars, most amino acids, organic acids or nucleotides are not retained by conventional reversed-phase LC columns and polar stationary phases and hydrophilic-interaction LC lacks of robustness, which is still limiting their applications for untargeted metabolomics where reproducibility is a must. Biological samples such as blood, urine or even tissues include many hydrophilic compounds secreted from cells, their analysis is essential for biomarker discovery, disease progression or treatment effects. This review focuses on CE coupled to MS as a mature technique for untargeted metabolomics including sample pretreatment, types of matrices, analytical methods, applications and data treatment strategies for polar compound analysis in biological matrices. The main applications and results of CE-MS in untargeted metabolomics are discussed and presented in a tabulated format.

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Metabolomic analysis of the effects of omeprazole and famotidine on aspirin-induced gastric injury

Cited by 13 publications

References 29 publications

Therapeutic effects of hydro-alcoholic extract of Achillea wilhelmsii C. Koch on indomethacin-induced gastric ulcer in rats: a proteomic and metabolomic approach

Therapeutic effects of hydro-alcoholic extract of Achillea wilhelmsii C. Koch on indomethacin-induced gastric ulcer in rats: a proteomic and metabolomic approach

Hydroxyproline, a Serum Biomarker Candidate for Gastric Ulcer in Rats: A Comparison Study of Metabolic Analysis of Gastric Ulcer Models Induced by Ethanol, Stress, and Aspirin

Capillary Electrophoresis Mass Spectrometry as A Tool for Untargeted Metabolomics

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