Metabolomic changes in grains of well‐watered and drought‐stressed transgenic rice

Nam, Kyong‐Hee; Shin, Hee Jae; Pack, In-Soon; Park, Jung‐Ho; Kim, Ho Bang; Kim, Chang-Gi

doi:10.1002/jsfa.7152

Cited by 39 publications

(24 citation statements)

References 46 publications

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“…Glucose and fructose are two of the main reducing sugars that are responsible for plant signal transduction to modulate drought tolerance [42]. Elevated level of glucose and fructose is involved in turgor maintenance [43] and associated with improved stress tolerance in many plants [44][45][46]. Mannose has been shown to be an important compatible osmolyte and play a role in protein glycosylation in plants [47].…”

Section: Exogenous Cor Promotes the Accumulation Of Carbohydrates Undmentioning

confidence: 99%

Combining Physiological and Metabolomic Analysis to Unravel the Regulations of Coronatine Alleviating Water Stress in Tobacco (Nicotiana tabacum L.)

Zhou

et al. 2020

Biomolecules

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Drought is a major abiotic stress that restricts plants growth, development, and yield. Coronatine (COR), a mimic of JA-Ile, functions in plant tolerance to multiple stresses. In our study, we examined the effects of COR in tobacco under polyethylene glycol (PEG) stress. COR treatment improved plant growth under stress as measured by fresh weight (FW) and dry weight (DW). The enzyme activity assay indicated that, under osmotic stress conditions, the activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR) were enhanced by COR treatment. Histochemical analyses via nitrotetrazolium blue chloride (NBT) and 3,3′-diaminobenzidine (DAB) staining showed that COR reduced reactive oxygen species (ROS) accumulation during osmotic stress. Metabolite profiles revealed that COR triggered significant metabolic changes in tobacco leaves under osmotic stress, and many essential metabolites, such as sugar and sugar derivatives, organic acids, and nitrogen-containing compounds, which might play active roles in osmotic-stressed tobacco plants, were markedly accumulated in the COR-treated tobacco. The work presented here provides a comprehensive understanding of the COR-mediated physiological, biochemical, and metabolic adjustments that minimize the adverse impact of osmotic stress on tobacco.

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Section: Exogenous Cor Promotes the Accumulation Of Carbohydrates Undmentioning

confidence: 99%

Combining Physiological and Metabolomic Analysis to Unravel the Regulations of Coronatine Alleviating Water Stress in Tobacco (Nicotiana tabacum L.)

Zhou

et al. 2020

Biomolecules

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“…2). The growth of root systems depends on the availability of carbon to the roots (Muller et al 1998). The processes of cell division and cell elongation are thought to rely on carbon availability, and carbon depletion can inhibit cell division (Van't Hof 1968;Green and Kehinde 1976).…”

Section: Understanding the Root Metabolite Profiling In Response To Rmentioning

confidence: 99%

Comparative metabolite profiling of two switchgrass ecotypes reveals differences in drought stress responses and rhizosheath weight

Liu

Chen

Zhang

et al. 2019

Planta

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“…Metabolite analysis via GC-MS was conducted as we have described previously [20]. At Weeks 0 (nondrought), 4 (mild-drought), and 9 (moderate-drought), two plants from each of the six replicated plots were collected and the samples were pooled from each plot to analyze metabolites.…”

Section: Metabolite Extraction and Gc-ms Analysismentioning

confidence: 99%

Global metabolite profiling based on GC–MS and LC–MS/MS analyses in ABF3-overexpressing soybean with enhanced drought tolerance

Nam

Kim

et al. 2019

Appl Biol Chem

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Abscisic acid (ABA) is a phytohormone that plays an important role in the adaptive responses to abiotic stresses. We examined the metabolic changes in transgenic soybean that over-expressed Arabidopsis ABA responsive elementbinding factor 3 (ABF3), which participates in drought tolerance. Transgenic and non-transgenic plants were exposed to a water deficit, and their metabolic differences were verified by untargeted GC-MS and LC-MS/MS analyses. A total of 64 and 476 primary and secondary metabolites from leaf extracts were identified based on GC-MS and LC-MS/MS platforms, respectively. Principal component analysis derived from both GC-MS and LC-MS/MS data showed a clearly greater separation in the metabolite profiles among three different degrees of drought stress. However, no discrimination of metabolites between transgenic and non-transgenic plants was apparent. Furthermore, except for some free amino acids, quantitative differences in relative levels of those metabolites were less than 50% between genotypes. These results suggest that, during periods of drought, overexpression of ABF3 in transgenic soybean might result in a negligible variance in primary and secondary metabolism when compared with its non-transgenic counterpart.

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Metabolomic changes in grains of well‐watered and drought‐stressed transgenic rice

Abstract: These changes in amounts of metabolites may assist in improving drought tolerance in transgenic rice by playing crucial roles in stress-responsive pathways including GABA biosynthesis, sucrose metabolism and antioxidant defenses.

Cited by 39 publications

References 46 publications

Combining Physiological and Metabolomic Analysis to Unravel the Regulations of Coronatine Alleviating Water Stress in Tobacco (Nicotiana tabacum L.)

Combining Physiological and Metabolomic Analysis to Unravel the Regulations of Coronatine Alleviating Water Stress in Tobacco (Nicotiana tabacum L.)

Comparative metabolite profiling of two switchgrass ecotypes reveals differences in drought stress responses and rhizosheath weight

Global metabolite profiling based on GC–MS and LC–MS/MS analyses in ABF3-overexpressing soybean with enhanced drought tolerance

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