Metabolomic Fingerprinting of Salinispora From Atlantic Oceanic Islands

Abstract

Salinispora (Micromonosporaceae) is an obligate marine bacterium genus consisting of three species that share over 99% 16S rRNA identity. The genome and biosynthetic pathways of the members of this genus have been widely investigated due to their production of species-specific metabolites. However, despite the species’ high genetic similarity, site-specific secondary metabolic gene clusters have been found in Salinispora strains collected at different locations. Therefore, exploring the metabolic expression of Salinispora recovered from different sites may furnish insights into their environmental adaptation or their chemical communication and, further, may lead to the discovery of new natural products. We describe the first occurrence of Salinispora strains in sediments from the Saint Peter and Saint Paul Archipelago (a collection of islets in Brazil) in the Atlantic Ocean, and we investigate the metabolic profiles of these strains by employing mass-spectrometry-based metabolomic approaches, including molecular networking from the Global Natural Products Social Molecular Networking platform. Furthermore, we analyze data from Salinispora strains recovered from sediments from the Madeira Archipelago (Portugal, Macaronesia) in order to provide a wider metabolomic investigation of Salinispora strains from the Atlantic Oceanic islands. Overall, our study evidences a broader geographic influence on the secondary metabolism of Salinispora than was previously proposed. Still, some biosynthetic gene clusters, such as those corresponding to typical chemical signatures of S. arenicola, like saliniketals and rifamycins, are highly conserved among the assessed strains.

The Essentials of Marine Biotechnology

“…Between 2007 and 2017, 177 new actinobacterial species belonging to 29 novel genera and three novel families were described (Subramani and Sipkema, 2019). The Gram-positive actinobacteria are a major chemically prolific source of bioactive metabolites with anticancer, antimicrobial, antiparasitic, anti-inflammatory, antibiofilm activities, and antifouling properties, among others (Prieto-Davó et al, 2016;Bauermeister et al, 2018Bauermeister et al, , 2019Cartuche et al, 2019Cartuche et al, , 2020Girão et al, 2019;Pereira et al, 2020). There are several marine-derived actinomycete metabolites described in the literature.…”

Diving Into Reef Ecosystems for Land-Agriculture Solutions: Coral Microbiota Can Alleviate Salt Stress During Germination and Photosynthesis in Terrestrial Plants

“…Salinispora arenicola is considered a strict marine bacterium, whose ecological role and relevance is still largely unknown. However, S. arenicola has recently gathered considerable attention due to its high proportion of specialized metabolite biosynthetic genes with promising biotechnological potential (Amos et al, 2017;Bauermeister et al, 2018).…”

Anticancer Potential of Compounds from the Brazilian Blue Amazon

“…Following this hint, further studies then confirmed the occurrence of Salinispora sp. at the SPSPA [81], which was the first report of this genus in Brazilian waters, and compared the metabolomics profile of strictly marine actinobacteria Salinospora arenicola and S. pacifica among strains occurring in Brazilian and Portuguese islands [152]. By using the spectral library search from GNPS, the authors showed that S. arenicola strains isolated from Brazilian waters are able to produce the molecular families of staurosporine, desferrioxamine, rifamycin, ferroxamine, and saliniketal, typical compounds to the metabolome of S. arenicola.…”

“…By using the spectral library search from GNPS, the authors showed that S. arenicola strains isolated from Brazilian waters are able to produce the molecular families of staurosporine, desferrioxamine, rifamycin, ferroxamine, and saliniketal, typical compounds to the metabolome of S. arenicola. Through inspection of the molecular networking, a new saliniketal analog with a difference of a methyl group was found [152].…”