Metabolomics analysis of the serum from children with urolithiasis using UPLC‐MS

Wen, Junxiang; Cao, Yingying; Li, Yang; Zhu, Fenhua; Yuan, Meifen; Xu, Jin; Li, Jian

doi:10.1111/cts.12984

Cited by 12 publications

(8 citation statements)

References 42 publications

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“…These results indicated that the metabolic phenotype of serum in patients with kidney stones was significantly different from that found in normal controls. The study provided a new understanding into the potential pathogenesis of urolithiasis, which may help to develop novel therapeutic strategies and preventive interventions [53]. Metabolomics can also be used to identify and quantify metabolites from plants.…”

Section: Application Of Uhplc In Pharmacokinetics and Bioavailability...mentioning

confidence: 99%

Principles and Applications of Ultra-High-Performance Liquid Chromatography

Ahmed¹,

Eticha²,

Hymete³

et al. 2023

High Performance Liquid Chromatography - Recent Advances and Applications

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The science of separation had advanced significantly with the development of ultra-high-performance liquid chromatography (UHPLC), a brand-new type of liquid chromatography. The need for the evolution of HPLC into UHPLC has been driven by the continuously evolving of packing material modifications that affect the separation of mixtures. The separation process of analytes is completed in a substantially decreased amount of time due to the lower particle sizes, which increases surface area of interaction allowing reduction of column length to one-third; thus, shorter columns are employed in UHPLC, which consequently causes the flow rate to be three times higher and subsequently reducing analysis time. Although UHPLC shares the same fundamental idea and instrument layout as HPLC, it differs from HPLC in that it produces narrow peaks and has high spectral quality, allowing for simple compound identification in a variety of analytical applications such as impurity profiling, product formulation, and improved analytical technique and method development. However, high back pressure in UHPLC might lead to decreased column life, and the instrument’s higher price compared to HPLC are the disadvantages.

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Section: Application Of Uhplc In Pharmacokinetics and Bioavailability...mentioning

confidence: 99%

Principles and Applications of Ultra-High-Performance Liquid Chromatography

Ahmed¹,

Eticha²,

Hymete³

et al. 2023

High Performance Liquid Chromatography - Recent Advances and Applications

View full text Add to dashboard Cite

show abstract

“…Wen et al [33] reported serum metabolomics assayed with LC-MS in 30 SF (10 CaOx, 10 cysteine, 10 calcium carbonate, mean age 5 AE 4) compared with 20 non-SF of similar age. Metabolites differed between SF and non-SF, and also between the three SF groups.…”

Section: Linking the Microbiome And Metabolome In Stone Formersmentioning

confidence: 99%

Metabolomic profiles and pathogenesis of nephrolithiasis

Kowalczyk,

Prochaska,

Worcester

2023

Current Opinion in Nephrology &Amp; Hypertension

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Purpose of review Kidney stone disease is caused by supersaturation of urine with certain metabolites and minerals. The urine composition of stone formers has been measured to prevent stone recurrence, specifically calcium, uric acid, oxalate, ammonia, citrate. However, these minerals and metabolites have proven to be unreliable in predicting stone recurrence. Metabolomics using high throughput technologies in well defined patient cohorts can identify metabolites that may provide insight into the pathogenesis of stones as well as offer possibilities in therapeutics. Recent findings Techniques including 1H-NMR, and liquid chromatography paired with tandem mass spectroscopy have identified multiple possible metabolites involved in stone formation. Compared to formers of calcium oxalate stones, healthy controls had higher levels of hippuric acid as well as metabolites involved in caffeine metabolism. Both the gut and urine microbiome may contribute to the altered metabolome of stone formers. Summary Although metabolomics has offered several potential metabolites that may be protective against or promote stone formation, the mechanisms behind these metabolomic profiles and their clinical significance requires further investigation.

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“…In the recent years, advances in the knowledge of the role of metabolism in the development and progression of this condition have been reported [ 87 ]. In particular, branches of omics such as metabolomics have investigated the change in metabolites linked to lithiasis [ 88 ].…”

Section: Metabolomics In Kidney Stone Diseasementioning

confidence: 99%

“…Wen et al [ 88 ] analyzed the change in metabolites in patients with urolithiasis through serum metabolomics based on ultra-performance liquid chromatography mass spectrometry. The authors investigated a total of 50 patients (30 children with kidney stones and 20 controls) enrolled between January 2018 and July 2019.…”

Section: Metabolomics In Kidney Stone Diseasementioning

confidence: 99%

“…The authors investigated a total of 50 patients (30 children with kidney stones and 20 controls) enrolled between January 2018 and July 2019. A statistically significant change in 40 serum metabolites was found in patients with kidney stones involving bilirubin, which was decreased, and molecules involved in retinol metabolism, such as steroid hormone biosynthesis, porphyrin, and chlorophyll metabolism, which were greatly increased [ 88 ].…”

Section: Metabolomics In Kidney Stone Diseasementioning

confidence: 99%

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New Insights from Metabolomics in Pediatric Renal Diseases

Riccio¹,

Valentino²,

Passaro³

et al. 2022

Children

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Renal diseases in childhood form a spectrum of different conditions with potential long-term consequences. Given that, a great effort has been made by researchers to identify candidate biomarkers that are able to influence diagnosis and prognosis, in particular by using omics techniques (e.g., metabolomics, lipidomics, genomics, and transcriptomics). Over the past decades, metabolomics has added a promising number of ‘new’ biomarkers to the ‘old’ group through better physiopathological knowledge, paving the way for insightful perspectives on the management of different renal diseases. We aimed to summarize the most recent omics evidence in the main renal pediatric diseases (including acute renal injury, kidney transplantation, chronic kidney disease, renal dysplasia, vesicoureteral reflux, and lithiasis) in this narrative review.

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Metabolomics analysis of the serum from children with urolithiasis using UPLC‐MS

Abstract: This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

Cited by 12 publications

References 42 publications

Principles and Applications of Ultra-High-Performance Liquid Chromatography

Principles and Applications of Ultra-High-Performance Liquid Chromatography

Metabolomic profiles and pathogenesis of nephrolithiasis

New Insights from Metabolomics in Pediatric Renal Diseases

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