Metabolomics in pesticide research and development: review and future perspectives

Aliferis, Konstantinos A.; Chrysayi-Tokousbalides, Maria

doi:10.1007/s11306-010-0231-x

Cited by 114 publications

(74 citation statements)

References 194 publications

(166 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…To date, a small number of metabolomics studies on plant-pathogen interactions have been published [8], [9], [10], [11], [12] and the research on the topic has been recently reviewed [13], [14], [15].…”

Section: Introductionmentioning

confidence: 99%

FT-ICR/MS and GC-EI/MS Metabolomics Networking Unravels Global Potato Sprout's Responses to Rhizoctonia solani Infection

Jabaji

2012

PLoS ONE

Self Cite

View full text Add to dashboard Cite

The complexity of plant-pathogen interactions makes their dissection a challenging task for metabolomics studies. Here we are reporting on an integrated metabolomics networking approach combining gas chromatography/mass spectrometry (GC/MS) with Fourier transform ion cyclotron resonance/mass spectrometry (FT-ICR/MS) and bioinformatics analyses for the study of interactions in the potato sprout-Rhizoctonia solani pathosystem and the fluctuations in the global metabolome of sprouts. The developed bioanalytical and bioinformatics protocols provided a snapshot of the sprout's global metabolic network and its perturbations as a result of pathogen invasion. Mevalonic acid and deoxy-xylulose pathways were substantially up-regulated leading to the biosynthesis of sesquiterpene alkaloids such as the phytoalexins phytuberin, rishitin, and solavetivone, and steroidal alkaloids having solasodine and solanidine as their common aglycons. Additionally, the perturbation of the sprout's metabolism was depicted in fluctuations of the content of their amino acids pool and that of carboxylic and fatty acids. Components of the systemic acquired resistance (SAR) and hypersensitive reaction (HR) such as azelaic and oxalic acids were detected in increased levels in infected sprouts and strategies of the pathogen to overcome plant defense were proposed. Our metabolic approach has not only greatly expanded the multitude of metabolites previously reported in potato in response to pathogen invasion, but also enabled the identification of bioactive plant-derived metabolites providing valuable information that could be exploited in biotechnology, biomarker-assisted plant breeding, and crop protection for the development of new crop protection agents.

show abstract

Section: Introductionmentioning

confidence: 99%

FT-ICR/MS and GC-EI/MS Metabolomics Networking Unravels Global Potato Sprout's Responses to Rhizoctonia solani Infection

Jabaji

2012

PLoS ONE

Self Cite

View full text Add to dashboard Cite

show abstract

“…These details can be found in more extensive reviews dealing only with metabolomics (e.g., Aliferis and Chysayi-Tokousbalides, 2011). One approach to metabolomics is to generate one-dimensional (1D) proton NMR ( 1 H NMR) spectral fingerprints of crude extracts, hoping that the resulting information can provide clues to the mode of action, by comparing with fingerprints of phytotoxins with known modes of action (e.g., Ott et al, 2003; Aliferis and Chysayi-Tokousbalides, 2006).…”

Section: Metabolomicsmentioning

confidence: 99%

Omics Methods for Probing the Mode of Action of Natural and Synthetic Phytotoxins

2013

View full text Add to dashboard Cite

For a little over a decade, omics methods (transcriptomics, proteomics, metabolomics, and physionomics) have been used to discover and probe the mode of action of both synthetic and natural phytotoxins. For mode of action discovery, the strategy for each of these approaches is to generate an omics profile for phytotoxins with known molecular targets and to compare this library of responses to the responses of compounds with unknown modes of action. Using more than one omics approach enhances the probability of success. Generally, compounds with the same mode of action generate similar responses with a particular omics method. Stress and detoxification responses to phytotoxins can be much clearer than effects directly related to the target site. Clues to new modes of action must be validated with in vitro enzyme effects or genetic approaches. Thus far, the only new phytotoxin target site discovered with omics approaches (metabolomics and physionomics) is that of cinmethylin and structurally related 5-benzyloxymethyl-1,2-isoxazolines. These omics approaches pointed to tyrosine amino-transferase as the target, which was verified by enzyme assays and genetic methods. In addition to being a useful tool of mode of action discovery, omics methods provide detailed information on genetic and biochemical impacts of phytotoxins. Such information can be useful in understanding the full impact of natural phytotoxins in both agricultural and natural ecosystems.

show abstract

“…Focusing on crop protection chemicals, a great effort has been given towards the development of crop protection agents safer for consumers and the environment and more efficient than the existing ones. Metabolomics has been so far a valuable tool for highthroughput screening of bioactive substances in order to discover those with high selectivity, unique modes of action and acceptable ecotoxicological/toxicological profiles [115].…”

Section: Herbicidesmentioning

confidence: 99%

Metabolomics - Useful Tool for Study of Plant Responses to Abiotic Stresses

Kráľová¹,

Jampílek²,

Ostrovský³

2012

Ecological Chemistry and Engineering S

View full text Add to dashboard Cite

Abiotic stresses are produced by inappropriate levels of physical components of the environment and cause plant injury through unique mechanisms that result in specific responses. Metabolomics is a relatively new approach aimed at improved understanding of metabolic networks and the subsequent biochemical composition of plants and other biological organisms. The paper is focused on the use of metabolomics, metabolic profiling and metabolic fingerprinting to study plant responses to some environmental stresses (eg elevated temperature, chilling and freezing, drought, high salinity, UV radiation, high ozone levels, nutrient deficiency, oxidative stress, herbicides and heavy metals). Attention is also devoted to the effects of some environmental factors on plants such as high or low levels of CO2 or different levels of irradiance. Alterations of plants metabolites due to multiple abiotic stresses (drought-heat, drought-salinity, elevated CO2-salinity) are analysed as well. In addition, metabolomic approach to study plant responses to some artificial abiotic stresses, mechanical stress or pulsed electric field-induced stress is discussed. The most important analytical methods applied in metabolomics are presented and perspectives of metabolomics exploitation in the future are outlined, too.

show abstract

Metabolomics in pesticide research and development: review and future perspectives

Cited by 114 publications

References 194 publications

FT-ICR/MS and GC-EI/MS Metabolomics Networking Unravels Global Potato Sprout's Responses to Rhizoctonia solani Infection

FT-ICR/MS and GC-EI/MS Metabolomics Networking Unravels Global Potato Sprout's Responses to Rhizoctonia solani Infection

Omics Methods for Probing the Mode of Action of Natural and Synthetic Phytotoxins

Metabolomics - Useful Tool for Study of Plant Responses to Abiotic Stresses

Contact Info

Product

Resources

About