Metabolomics in the Developing Human Being

Syggelou, Angeliki; Iacovidou, Nicoletta; Atzori, Luigi; Xanthos, Theodoros; Fanos, Vassilios

doi:10.1016/j.pcl.2012.07.002

Cited by 33 publications

(18 citation statements)

References 75 publications

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“…Specifically, metabolomics is the "systematic study of the unique chemical fingerprints that specific cellular processes leave behind," that is, the study of their small-molecule metabolite profiles (Syggelou et al, 2012). The metabolome represents the collection of all metabolites, which are the end products of cellular processes in a biological cell, tissue, organ, or organism (Jordan et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Abnormal metabolites related to bone marrow failure in aplastic anemia patients

Zhong¹,

Zhang²,

Cui³

2015

Genet. Mol. Res.

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ABSTRACT. Metabolomics is the identification and quantitation of small molecules that are involved in metabolic reactions. Liquid chromatographymass spectrometry (LC-MS) has enjoyed growing popularity as the platform for metabolomic studies due to high throughput, soft ionization, and good coverage of metabolites. Serum metabolites of aplastic anemia (AA) patients and healthy controls were investigated using LC-MS. A wavelet-based method was utilized to find and align LC-MS peaks. Principal component analysis, partial least squares discriminant analysis, and optimized potential for liquid simulations were used to identify differences in metabolite levels, and to reveal useful biomarkers. Thirtytwo metabolites that were significantly altered were detected. Of these metabolites, 23 were successfully identified. In AA patients, metabolites involved amino acid biosynthesis, aminoacyl-tRNA biosynthesis, and ATPbinding cassette transporters were higher than normal, while the levels of metabolites involved in TCA cycles were lower than normal. These 13709-13718 (2015) changes may be the primary cause or result of bone marrow failure in patients with AA.

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Section: Introductionmentioning

confidence: 99%

Abnormal metabolites related to bone marrow failure in aplastic anemia patients

Zhong¹,

Zhang²,

Cui³

2015

Genet. Mol. Res.

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“…Hence, metabolic profiling can illustrate the instantaneous pathological or physiological changes as a supplement to proteomic and transcriptomic profiling for the systematic study of living organisms’ functional status [6–8]. Many studies reveal that the advent of some kinds of cancer is accompanied by metabolic changes, and specific small molecule metabolites profiling can distinguish different clinical and pathological characteristics of cancer such as breast cancer, colon cancer, oral cancer, and prostate cancer [9–15].…”

Section: Introductionmentioning

confidence: 99%

Identification of Plasma Metabolomic Profiling for Diagnosis of Esophageal Squamous-Cell Carcinoma Using an UPLC/TOF/MS Platform

Liu

Yuan

et al. 2013

IJMS

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Epidemiological studies indicated that esophageal squamous-cell carcinoma (ESCC) is still one of the most common causes of cancer incidence in the world. Searching for valuable markers including circulating endogenous metabolites associated with the risk of esophageal cancer, is extremely important A comparative metabolomics study was performed by using ultraperformance liquid chromatography-electrospray ionization-accurate mass time-of-flight mass spectrometry to analyze 53 pairs of plasma samples from ESCC patients and healthy controls recruited in Huaian, China. The result identified a metabolomic profiling of plasma including 25 upregulated metabolites and five downregulated metabolites, for early diagnosis of ESCC. With a database-based verification protocol, 11 molecules were identified, and six upregulated molecules of interest in ESCC were found to belong to phospholipids as follows: phosphatidylserine, phosphatidic acid, phosphatidyl choline, phosphatidylinositol, phosphatidyl ethanolamine, and sphinganine 1-phosphate. Clinical estimation of metabolic biomarkers through hierarchical cluster analysis in plasma samples from 17 ESCC patients and 29 healthy volunteers indicated that the present metabolite profile could distinguish ESCC patients from healthy individuals. The cluster of aberrant expression of these metabolites in ESCC indicates the critical role of phospholipid metabolism in the oncogenesis of ESCC and suggests its potential ability to assess the risk of ESCC development in addition to currently used risk factors.

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“…Accordingly, it can be considered that the metabolome delivers a dynamic snapshot of the functional level of a biological system more appropriate than those provided either by genomics or proteomics [12], [13]. The increasing use of metabolomics to gain further insight into the medical conditions affecting the neonate and the developing human being [14] as well as in different areas of neonatology has been recently reviewed [15]. In a previous targeted metabolomic study it was shown that plasma ratios of alanine to branched-chained amino acids (BCAA) and of glycine to BCAA more accurately reflected duration and intensity of hypoxia in newborn piglets than the traditionally employed plasma lactate concentration [1].…”

Section: Introductionmentioning

confidence: 99%

Metabolomic Analysis of the Effect of Postnatal Hypoxia on the Retina in a Newly Born Piglet Model

et al. 2013

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The availability of reliable biomarkers of brain injury secondary to birth asphyxia could substantially improve clinical grading, therapeutic intervention strategies, and prognosis. In this study, changes in the metabolome of retinal tissue caused by profound hypoxia in an established neonatal piglet model were investigated using an ultra performance liquid chromatography – quadrupole time of flight mass spectrometry (UPLC-QTOFMS) untargeted metabolomic approach, which included Partial Least Squares – Discriminant Analysis (PLSDA) multivariate data analysis. The initial identification of a set of discriminant metabolites from UPLC-QTOFMS data was confirmed by target UPLC-MS/MS and allowed the selection of endogenous CDP-choline as a promising candidate biomarker for hypoxia-derived brain damage assessing intensity of retinal hypoxia. Results from this study will foster further research on CDP-choline changes occurring during resuscitation.

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Metabolomics in the Developing Human Being

Cited by 33 publications

References 75 publications

Abnormal metabolites related to bone marrow failure in aplastic anemia patients

Abnormal metabolites related to bone marrow failure in aplastic anemia patients

Identification of Plasma Metabolomic Profiling for Diagnosis of Esophageal Squamous-Cell Carcinoma Using an UPLC/TOF/MS Platform

Metabolomic Analysis of the Effect of Postnatal Hypoxia on the Retina in a Newly Born Piglet Model

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