Metabolomics of the Effect of 1-MCP and Ca During Ripening and Storage in Apples

Bekele, Elias Abebe; Beshir, Wasiye Fikremariam; Hertog, Maarten; Nicolaı̈, Bart; Geeraerd, Annemie

doi:10.17660/actahortic.2015.1079.25

Cited by 5 publications

(2 citation statements)

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“…For example, alanine and aspartic acid were transformed into pyruvate and oxaloacetic acid, respectively. Other amino acids, such as valine, leucine, isoleucine, and lysine, were transformed into a-ketoisocaproic acid through the combined transamination (Bekele et al, 2015;Ren et al, 2020). The GS and ADC in toon buds at RT storage exhibited significantly higher expressions than those at LT storage.…”

Section: Changes In the Sugar Metabolism Of Toon Buds During Storage ...mentioning

confidence: 99%

“…This finding indicated that the glyoxylic acid cycle involved in fatty acid catabolism was also strengthened. Intermediate accumulation in the EMP−TCA pathway strengthened gluconeogenesis, transforming non-sugar substances into sugars such as fructose and sucrose (Bekele et al, 2015;Liu et al, 2016).…”

Section: Changes In the Sugar Metabolism Of Toon Buds During Storage ...mentioning

confidence: 99%

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Metabolic profiling and gene expression analysis reveal the quality deterioration of postharvest toon buds between two different storage temperatures

et al. 2023

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Toon buds, a popular woody vegetable, contain large amounts of nutrients. However, toon buds have strong respiratory metabolism after harvest and are highly prone to decay, resulting in quality deterioration. Low temperature can effectively inhibit postharvest senescence of toon buds. GC-TOF-MS combined with quantitative real-time PCR was used to elucidate the toon bud deterioration mechanism after harvest by analyzing the difference in the relative contents of primary metabolites and their derivatives, and the expression of key genes associated with metabolic pathways in toon buds between low temperature and room temperature storages for 72 h. Results showed that the ethylene synthesis in toon buds accelerated under room temperature storage, along with significant changes in the primary metabolic pathway. The catabolism of amino acids, fatty acids, and cell membrane phospholipids was accelerated, and the gluconeogenesis synthesis was strengthened. Moreover, the sucrose synthesis was increased, the glycolysis and TCA cycle were broken down, and the pentose phosphate pathway was vigorous. As metabolic intermediates, organic acids were considerably accumulated. Moreover, varieties of toxic compounds were produced in parallel with the activation of aromatic compounds. This work provided a comprehensive understanding of the metabolic regulation, thereby revealing how low and room temperatures differentially influenced the quality deterioration of postharvest toon buds.

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Section: Changes In the Sugar Metabolism Of Toon Buds During Storage ...mentioning

confidence: 99%

Section: Changes In the Sugar Metabolism Of Toon Buds During Storage ...mentioning

confidence: 99%

Metabolic profiling and gene expression analysis reveal the quality deterioration of postharvest toon buds between two different storage temperatures

et al. 2023

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Impact of pre-harvest application of 1-methylcyclopropene (1-MCP) on fruit quality, physiological disorders and respiration rate of ‘Scilate’ apple

Sidhu,

Bound,

Hunt

et al. 2024

Hortic. Environ. Biotechnol.

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The ethylene inhibitor 1-methylcyclopropene (1-MCP) is widely used in the apple industry as a post-harvest fumigation application to slow down the ripening process and maintain fruit quality during storage. With the development of a sprayable formulation (Harvista™, Agrofresh Inc, USA), it is becoming increasingly common in the apple industry to apply 1-MCP as a pre-harvest spray. This study investigated the impact of pre-harvest 1-MCP applied 1 week prior to harvest as a foliar application at 11.4 L ha−1 in 1000 L water, on the quality and respiration rate of ‘Scilate’ apples grown in the Huon Valley, Southern Tasmania, Australia. Two trials were undertaken, trial 1 examined fruit quality, flesh browning (FB) and fruit softening in 1-MCP treated and untreated fruit while trial 2 compared the respiration rate of 1-MCP treated and untreated fruit. Despite no effect on fresh fruit quality attributes, pre-harvest spray application of 1-MCP influenced post-harvest fruit quality, storability, and respiration rate of apples. Fruit treated with 1-MCP showed higher malic acid concentration (1.2 g L−1 vs 0.9 g L−1) and lower juice pH (4.6 vs 4.9) even after 7.5 months of regular air cold-storage along with a six-fold reduction in fruit softening in comparison to untreated fruit (2% vs 13%). Pre-harvest treatment of 1-MCP reduced respiration rate by 32%, and incidence of CO2 injury by over 40%. The incidence of radial type (senescent related) FB was reduced by 100%. A significant proportion of treated apples still suffered from severe CO2 injury. This study has demonstrated that pre-harvest application of 1-MCP to ‘Scilate’ fruit can be useful in maintaining long-term fruit quality during storage, reducing respiration rate, and lowering the incidence of FB in general. However, the risk of severe CO2 injury remains, especially with larger fruit harvested from trees with lighter crop loads.

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Classification of 1-methylcyclopropene treated apples by fluorescence fingerprint using partial least squares discriminant analysis with stepwise selectivity ratio variable selection method

Trivittayasil

Tsuta

Kasai

et al. 2018

Chemometrics and Intelligent Laboratory Systems

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Metabolomics of the Effect of 1-MCP and Ca During Ripening and Storage in Apples

Cited by 5 publications

References 0 publications

Metabolic profiling and gene expression analysis reveal the quality deterioration of postharvest toon buds between two different storage temperatures

Metabolic profiling and gene expression analysis reveal the quality deterioration of postharvest toon buds between two different storage temperatures

Impact of pre-harvest application of 1-methylcyclopropene (1-MCP) on fruit quality, physiological disorders and respiration rate of ‘Scilate’ apple

Classification of 1-methylcyclopropene treated apples by fluorescence fingerprint using partial least squares discriminant analysis with stepwise selectivity ratio variable selection method

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