Metagenome-wide association study of the alterations in the intestinal microbiome composition of ankylosing spondylitis patients and the effect of traditional and herbal treatment

Huang, Run‐Yue; Li, Fang; Zhou, Yingyan; Zeng, Zhenhua; He, Xinrong; Fang, Lihua; Pan, Feng; Chen, Yile; Lin, Jiehua; Li, Jie; Qiu, Dongni; Tian, Yinping; Tan, Xi; Song, Yanni; Xu, Yong-Yue; Lai, Yonghui; Hao, Yi; Gao, Qiang; Fang, Xiaodong; Shi, Mingming; Zhou, Chu; Huang, Jinqun; He, Ying

doi:10.1099/jmm.0.001107

Cited by 27 publications

(17 citation statements)

References 36 publications

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“…For example, F. prausnitzii, a major butyrate producer that is depleted in many chronic diseases 9 , shows significant co-abundance with the majority of Bacteroidetes and Bifidobacterium species (Supplementary Table 1e). However, we also identified potential keystone species with low prevalence (≤10%), including Ruminococcus gnavus and multiple species from the genus Clostridium, that are positively associated with multiple diseases (Supplementary Table 3b), consistent with previous studies 10,11 .…”

Section: Core and Keynote Speciessupporting

confidence: 87%

Environmental factors shaping the gut microbiome in a Dutch population

Gaćeša

Kurilshikov

Vila

et al. 2022

Nature

391

266

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Section: Core and Keynote Speciessupporting

confidence: 87%

Environmental factors shaping the gut microbiome in a Dutch population

Gaćeša

Kurilshikov

Vila

et al. 2022

Nature

391

266

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“…While we focus our metagenomics conclusions on the tryptophan pathway, due to the consistency with our metabolomics analyses, we do observe similar metagenomics pathways in comparison with previously published results. For example, in agreement with other’s findings of the TCA cycle and biotin synthesis being enriched in AS, and butanoate pathways and pyridoxal 5’-phosphate salvage pathways being enriched in controls ( 47 , 50 , 51 ). These studies focused on untreated AS, but when comparing untreated to TNFi treated AS cases, not only did the microbiome become less significantly different from controls, so did metagenomic findings ( 47 ).…”

Section: Discussionsupporting

confidence: 90%

Multi ‘Omics Analysis of Intestinal Tissue in Ankylosing Spondylitis Identifies Alterations in the Tryptophan Metabolism Pathway

Berlinberg

Regner²,

Stahly

et al. 2021

Front. Immunol.

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Intestinal microbial dysbiosis, intestinal inflammation, and Th17 immunity are all linked to the pathophysiology of spondyloarthritis (SpA); however, the mechanisms linking them remain unknown. One potential hypothesis suggests that the dysbiotic gut microbiome as a whole produces metabolites that influence human immune cells. To identify potential disease-relevant, microbiome-produced metabolites, we performed metabolomics screening and shotgun metagenomics on paired colon biopsies and fecal samples, respectively, from subjects with axial SpA (axSpA, N=21), Crohn’s disease (CD, N=27), and Crohn’s-axSpA overlap (CD-axSpA, N=12), as well as controls (HC, N=24). Using LC-MS based metabolomics of 4 non-inflamed pinch biopsies of the distal colon from subjects, we identified significant alterations in tryptophan pathway metabolites, including an expansion of indole-3-acetate (IAA) in axSpA and CD-axSpA compared to HC and CD and indole-3-acetaldehyde (I3Ald) in axSpA and CD-axSpA but not CD compared to HC, suggesting possible specificity to the development of axSpA. We then performed shotgun metagenomics of fecal samples to characterize gut microbial dysbiosis across these disease states. In spite of no significant differences in alpha-diversity among the 4 groups, our results confirmed differences in gene abundances of numerous enzymes involved in tryptophan metabolism. Specifically, gene abundance of indolepyruvate decarboxylase, which generates IAA and I3Ald, was significantly elevated in individuals with axSpA while gene abundances in HC demonstrated a propensity towards tryptophan synthesis. Such genetic changes were not observed in CD, again suggesting disease specificity for axSpA. Given the emerging role of tryptophan and its metabolites in immune function, altogether these data indicate that tryptophan metabolism into I3Ald and then IAA is one mechanism by which the gut microbiome potentially influences the development of axSpA.

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“…This suggests a possible role for P. distasonis in the development of more diverse autoimmune responses. 112,113 In vitro experiments showed that P. distasonis, along with other AS-enriched species including Bacteroides coprophilus, Eubacterium siraeum, Acidaminococcus fermentans, and Prevotella copri, increased the amount of IFN-γ-producing cells through a bacterial peptide of these species, mimicking type II collagen and likely serving as "triggers of autoimmunity by molecular mimicry". 105,110,114 In psoriatic patients, P. distasonis presence was found to be significantly decreased relative to nonpsoriatic patients.…”

Section: Gut Microbiome and Dichotomous Role On Autoimmune Diseasesmentioning

confidence: 99%

Parabacteroides distasonis: intriguing aerotolerant gut anaerobe with emerging antimicrobial resistance and pathogenic and probiotic roles in human health

et al. 2021

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Parabacteroides distasonis is the type strain for the genus Parabacteroides, a group of gram-negative anaerobic bacteria that commonly colonize the gastrointestinal tract of numerous species. First isolated in the 1930s from a clinical specimen as Bacteroides distasonis, the strain was re-classified to form the new genus Parabacteroides in 2006. Currently, the genus consists of 15 species, 10 of which are listed as 'validly named' (P. acidifaciens, P. chartae, P. chinchillae, P. chongii, P. distasonis, P. faecis, P. goldsteinii, P. gordonii, P. johnsonii, and P. merdae) and 5 'not validly named' (P. bouchesdurhonensis, P. massiliensis, P. pacaensis, P. provencensis, and P. timonensis) by the List of Prokaryotic names with Standing in Nomenclature. The Parabacteroides genus has been associated with reports of both beneficial and pathogenic effects in human health. Herein, we review the literature on the history, ecology, diseases, antimicrobial resistance, and genetics of this bacterium, illustrating the effects of P. distasonis on human and animal health.

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Metagenome-wide association study of the alterations in the intestinal microbiome composition of ankylosing spondylitis patients and the effect of traditional and herbal treatment

Cited by 27 publications

References 36 publications

Environmental factors shaping the gut microbiome in a Dutch population

Environmental factors shaping the gut microbiome in a Dutch population

Multi ‘Omics Analysis of Intestinal Tissue in Ankylosing Spondylitis Identifies Alterations in the Tryptophan Metabolism Pathway

Parabacteroides distasonis: intriguing aerotolerant gut anaerobe with emerging antimicrobial resistance and pathogenic and probiotic roles in human health

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