Metagenomic Analysis of Intestinal Microbiota in Florated Rats

Abstract

Changes in gut microbiota have shown that it plays an important role in animal health and metabolic diseases. The intestinal microbiota is a complex structure that functions as an organ system with the presence of trillions of microorganisms. In this study, changes in the intestinal microbiota of Wistar rats with high fluorine were evaluated. Water containing 100 ppm NaF was given to 14 male Wistar albino rats as drinking water for 12 weeks. Fluorine is known to be an inducer of protein oxidation, lipid peroxidation, modulation of intracellular redox homeostasis, and oxidative stress. In this study, it was determined that the level of MDA (molandialdehyde), one of the oxidative stress parameters, increased significantly in the intestinal tissue after fluorine intoxication. The decrease in CAT (catalase) and SOD (superoxide dismutase) enzyme activities was found to be statistically significant. Intestinal tissues were taken under aseptic conditions and microorganisms found in flora were replicated by V3-V4 16S rRNA gene-specific primers. As a result of the sequence analysis, a statistical comparison of the control group and the fluorine applied group was made. The study we have done showed that there was a significant difference in species diversity in the intestinal microbiota of mice treated with fluorine. As a result, the composition of the intestinal microflora, especially Lactobacillus species, was significantly changed in rats with high fluorine.

The Effect of Chitosan and Chitosan Oligosaccharide on Serum Mineral and Vitamin Levels in Rats with Experimental Fluorosis

“…However, it has harmful effects on the liver and kidneys as well as other tissues and organs (Tkachenko et al 2021;Çetin et al 2019). Studies by many researchers have shown that oxygen radical formation and lipid peroxidation are among the harmful effects of chronic fluorosis (Chlubek and Poland 2003;Yur et al 2013;Komuroglu et al 2022). It has been reported that the use of antioxidants and antioxidantrich foods can act as an antidote in the treatment of fluorosis in the prevention of oxidative stress-induced lipid peroxidation (Samal et al 2016;Mesram et al 2017).…”

ANOM Approach for Statistical Evaluation of Some Antioxidant Enzyme Activities

“…Superoxide dismutase (SOD) is present in all cells that use oxygen and is aerobic. It plays an important role in defense against the superoxide radical formed as a result of the reactions (Komuroglu et al, 2021). SOD enzyme accelerates the reaction four times in biological systems (Michiels et al, 1994).…”