Metallo-β-lactamase and genetic diversity of Pseudomonas aeruginosa in intensive care units in Campo Grande, MS, Brazil

Rodrigues, Ana Cláudia Souza; Chang, Marilene Rodrigues; Nóbrega, Gabriela Dorn; Rodrigues, Mayara Souza; Carvalho, Nádia Cristina Pereira; Gomes, Beatriz Garcia; Almeida, Dênio Lopes de; Carvalho, Karyne Rangel; Asensi, Marise Dutra

doi:10.1016/s1413-8670(11)70174-x

Cited by 5 publications

(5 citation statements)

References 24 publications

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“…Hospitalized patients, particularly those who were admitted to the ICU, are mostly at risk for P. aeruginosa life-threatening infections [ 23 ]. Owing to the fact that imipenem resistance genes are usually located in the transposable genetic elements, along with other antibiotic resistance genes [ 24 ], the emergence of imipenem-resistant isolates of P. aeruginosa in healthcare settings is a serious problem.…”

Section: Discussionmentioning

confidence: 99%

Clonal Relatedness among Imipenem-ResistantPseudomonas aeruginosaIsolated from ICU-Hospitalized Patients

Vaez

Moghim

Esfahani

et al. 2015

Critical Care Research and Practice

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Imipenem-resistant Pseudomonas aeruginosa (P. aeruginosa) has become an increasingly important problem in healthcare settings worldwide. The aim of the present study was to evaluate clonal spread among imipenem-resistant P. aeruginosa isolated from ICU-hospitalized patients. Totally, 150 wound specimens were analyzed. Antibiotic resistance profiles and clonal diversity were evaluated using Kirby-Bauer's disk diffusion method and Random Amplified Polymorphic DNA- (RAPD-) PCR, respectively. The isolates showed a high frequency of antibiotic resistance against meropenem, and imipenem (100%) followed by ciprofloxacin, and ceftazidime (90%); meanwhile resistance to polymyxin B was not observed. Eighteen (40%) of P. aeruginosa isolates were MBL-positive via ethylenediaminetetraacetic acid (EDTA) combined disk test. Our findings showed high genetic diversity, with 37 different RAPD types detected. RAPD typing results showed cross-acquisition of P. aeruginosa in investigated hospital, suggesting failure in infection control practices. Incidence of MBL-positive isolates is high and should be regarded as a threat to hospitalized patients.

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Section: Discussionmentioning

confidence: 99%

Clonal Relatedness among Imipenem-ResistantPseudomonas aeruginosaIsolated from ICU-Hospitalized Patients

Vaez

Moghim

Esfahani

et al. 2015

Critical Care Research and Practice

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“…Pseudomonas aeruginosa is a Gram-negative, glucose non-fermentative bacillus that is often involved in nosocomial infection outbreaks (1). This opportunistic pathogen can cause a variety of infections including pneumonia, septicemia, meningitis, urinary tract infections, and burn wound infections (2,3).…”

Section: Introductionmentioning

confidence: 99%

Detection of Metallo-Beta-Lactamase-Encoding Genes Among Clinical Isolates of Pseudomonas aeruginosa in Qom Province

Meimani

Nazari

Rostami

2018

Zahedan J Res Med Sci

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Background: Carbapenem-resistant Pseudomonas aeruginosa has emerged as a serious problem in many hospitals and intensive care units. The purpose of this study was to determine the antibiotic susceptibility profile and the distribution of metallo-betalactamase genes among P. aeruginosa isolates from Qom province of Iran. Methods: In this study, 200 P. aeruginosa isolates were collected from several clinical samples in hospitals affiliated to Qom University of Medical Sciences, Qom province, Iran. The antibiotic susceptibility of the isolates was tested by the Kirby-Bauer disc diffusion test and the distribution of MBL genes among carbapenem-resistant isolates was determined by polymerase chain reaction (PCR) method. Results: Among carbapenem non-susceptible isolates of P. aeruginosa, 38% (76 isolates) were multidrug resistant, 26% (52 isolates) were pan-drug-resistant and 5% (10 isolates) were extensive drug resistant. 52% of the isolates were resistant to carbapenems. Among carbapenem non-susceptible isolates of P. aeruginosa, 40 isolates (38.4%) exhibited MBL production. Of 40 MBL-producing isolates, 38 isolates (36.5%) contained the blaIMP gene and two (1.9%) isolates contained the blaVIM gene. Conclusions: Outbreaks of MBL-producing P. aeruginosa isolates will be a serious problem in hospitals in the future and rapid identification of these isolates is necessary to control further dissemination of MBL resistance genes. This is the first report of the rates of MDR, XDR, PDR, and MBL-producing P. aeruginosa isolated from hospitals affiliated to Qom University of Medical Sciences in Qom province of Iran.

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“…Pseudomonas aeruginosa is a gram-negative, motile, aerobic and opportunistic hospital pathogen, which is sometimes resulting in deadly infections in their own host. [ 1 ] In addition, it is one of the infectious factors in patients with cystic fibrosis and it can also cause cancer and other diseases. [ 2 3 4 ]…”

Section: Introductionmentioning

confidence: 99%

“…[ 5 ] Metallo-beta-lactamase hydrolyses wide range of beta-lactamase (antibiotics, anti-cell wall) including cephalosporins, carbapenems and penicillins. [ 1 6 ] It also causes resistance to aminoglycosides. However, it is unable to hydrolyse monobactem like Aztreonam.…”

Section: Introductionmentioning

confidence: 99%

“…However, it is unable to hydrolyse monobactem like Aztreonam. [ 1 ] Metallo-beta-lactamase requires the Zn metal cofactor for its catalytic activity that is inhibited by EDTA and thiol compounds. Carbapenamase class B are designed of metallo-beta-lactamase and are divided in 6 groups based on the molecular structure including AIM, APM, GIM, SIM, IMP and VIM ; which the most common ones are IMP and VIM .…”

Section: Introductionmentioning

confidence: 99%

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Antibiotic resistance pattern and evaluation of metallo-beta lactamase genes (VIM and IMP) in Pseudomonas aeruginosa strains producing MBL enzyme, isolated from patients with secondary immunodeficiency

2016

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Background:One of the most common causes of hospital-acquired secondary infections in hospitalized patients is Pseudomonas aeruginosa. The aim of this study is to evaluate the expression of IMP and VIM in Pseudomonas aeruginosa strains (carbapenem resistant and producer MBL enzyme) in patients with secondary immunodeficiency.Materials and Methods:In a cross sectional study, 96 patients with secondary immunodeficiency hospitalized in the Al-Zahra hospital were selected. Carbapenem resistant strains isolated and modified Hodge test was performed in order to confirm the presence of the metallo carbapenemase enzyme. Under the standard conditions they were sent to the central laboratory for investigating nosocomial infection Multiplex PCR.Results:Of 96 samples 28.1% were IMP positive, 5.2% VIM positive and 3.1% both VIM and IMP positive. The prevalence of multidrug resistance in the IMP and/or VIM negative samples was 29%, while all 5 VIM positive samples have had multidrug resistance. Also the prevalence of multi-drug resistance in IMP positive samples were 96.3% and in IMP and VIM positive samples were 100%. According to Fisher’s test, the prevalence of multi-drug resistance based on gene expression has significant difference (P < 0.001).Conclusion:Based on the results of this study it can be concluded that, a significant percentage of patients with secondary immunodeficiency that suffer nosocomial infections with multidrug resistance, especially Pseudomonas aeruginosa, are probably MBL-producing gene positive. Therefore the cause of infection should be considered in the hospital care system to identify their features, the presence of genes involved in the development of multi-drug resistance and antibiotic therapy.

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Metallo-β-lactamase and genetic diversity of Pseudomonas aeruginosa in intensive care units in Campo Grande, MS, Brazil

Cited by 5 publications

References 24 publications

Clonal Relatedness among Imipenem-ResistantPseudomonas aeruginosaIsolated from ICU-Hospitalized Patients

Clonal Relatedness among Imipenem-ResistantPseudomonas aeruginosaIsolated from ICU-Hospitalized Patients

Detection of Metallo-Beta-Lactamase-Encoding Genes Among Clinical Isolates of Pseudomonas aeruginosa in Qom Province

Antibiotic resistance pattern and evaluation of metallo-beta lactamase genes (VIM and IMP) in Pseudomonas aeruginosa strains producing MBL enzyme, isolated from patients with secondary immunodeficiency

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