Methicillin-resistant Staphylococcus aureus in swine housed indoors in Galicia, Spain

Moreno-Flores, Antonio; Potel, Carmen; Francisco-Tomé, Mónica; Constenla-Caramés, Lucía; Pérez-Roth, Eduardo; López-Cotón, Carolina; Vila, Estefanía Comesaña-Da; Puente, Lourdes Eiroa-de la; Álvarez-Fernández, Maximiliano

doi:10.1016/j.eimce.2019.03.014

Cited by 3 publications

(5 citation statements)

References 27 publications

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Section: Discussionmentioning

confidence: 61%

“…As displayed by the results, a remarkable level of resistance to TET ( Figure 2 ) and the presence of TET resistance-associated genes ( Table 3 ), reported to be virtually present in all LA-MRSA [ 56 , 57 ], was demonstrated. This further corroborated data previously reported by several authors regarding TET resistance among LA-MRSA from pigs [ 18 , 22 , 23 , 51 , 57 ]. The main reason behind such TET-resistance levels is the intensive use of this antibiotic in farming [ 58 , 59 , 60 ] that may favour a cohort selection of TET-resistant bacteria; this is significantly notable in ST398 [ 21 , 53 ].…”

Section: Discussionmentioning

confidence: 61%

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Assessment of the Antibiotic Resistance Profile, Genetic Heterogeneity and Biofilm Production of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from The Italian Swine Production Chain

Rodríguez-López

Filipello

Ciccio

et al. 2020

Foods

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The main aim of the present study was to evaluate the level of antibiotic resistance, prevalence and virulence features of methicillin-resistant Staphylococcus aureus (MRSA) isolated from heavy swine at abattoir level and farming environments in Lombardy (Northern Italy). With this scope, 88 different heavy swine farms were surveyed, obtaining a total of n = 440 animal swabs and n = 150 environmental swabs. A total of n = 87 MRSA isolates were obtained, with an overall MRSA incidence of 17.50% (n = 77) among animal samples and a 6.67% (n = 10) among environmental. Molecular characterisation using multilocus sequence typing (MLST) plus spa-typing showed that sequence type ST398/t899 and ST398/t011 were the most commonly isolated genotypes, although other relevant sequence types such as ST1 or ST97 were also found. A lack of susceptibility to penicillins, tetracycline and ceftiofur was detected in >91.95, 85.05 and 48.28% of the isolates, respectively. Resistance to doxycycline (32.18%), enrofloxacin (27.59%) and gentamicin (25.29%) was also observed. Additionally, a remarkable level of antibiotic multiresistance (AMR) was observed representing a 77.01% (n = 67) of the obtained isolates. Genetic analysis revealed that 97.70% and 77.01% of the isolates harboured at least one antibiotic resistance or enterotoxin gene, respectively, pointing out a high isolate virulence potential. Lastly, 55.17% (n = 48) were able to produce measurable amounts of biofilm after 24 h. In spite of the current programmes for antibiotic reduction in intensively farming, a still on-going high level of AMR and virulence potential in MRSA was demonstrated, making this pathogen a serious risk in swine production chain, highlighting once more the need to develop efficient, pathogen-specific control strategies.

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Section: Discussionmentioning

confidence: 61%

Section: Discussionmentioning

confidence: 61%

Assessment of the Antibiotic Resistance Profile, Genetic Heterogeneity and Biofilm Production of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from The Italian Swine Production Chain

Rodríguez-López

Filipello

Ciccio

et al. 2020

Foods

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“…However, in one study, Moreno-Flores et al [107] observed an increase in LA-MRSA colonization in the noses of pigs fed with a zinc-supplemented diet [160]. In another study, zinc concentration in the feed was found to affect the colonization status of LA-MRSA in pigs [57].…”

Section: Use Of Antimicrobials and Zincmentioning

confidence: 94%

“…However, antibiotic treatment had no significant effect on the LA-MRSA-positive pigs [58,77]. The effect of antibiotic use is supported by many studies on LA-MRSA intervention [154,155] and transmission [37,107].…”

Section: Use Of Antimicrobials and Zincmentioning

confidence: 95%

“…Livestock-associated-methicillin-resistant S. aureus can also be transmitted from sows to piglets [100,[104][105][106]]. An experimental study demonstrated the transmission of LA-MRSA from sows to all newborn piglets [107]. Moreover, other studies also reported that piglets from LA-MRSA-positive sows tended to become LA-MRSA-positive [105,106].…”

Section: Pig-pig Transmissionmentioning

confidence: 99%

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A review of new emerging livestock-associated methicillin-resistant Staphylococcus aureus from pig farms

et al. 2023

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Methicillin-resistant Staphylococcus aureus (MRSA) is a S. aureus strain resistant to β-lactam antibiotics and is often associated with livestock, known as livestock-associated (LA)-MRSA. Using molecular typing with multi-locus sequence typing, MRSA clones have been classified in pigs, including clonal complex 398. Livestock-associated-methicillin-resistant S. aureus was first discovered in pigs in the Netherlands in 2005. Since then, it has been widely detected in pigs in other countries. Livestock-associated-methicillin-resistant S. aureus can be transmitted from pigs to pigs, pigs to humans (zoonosis), and humans to humans. This transmission is enabled by several risk factors involved in the pig trade, including the use of antibiotics and zinc, the size and type of the herd, and the pig pen management system. Although LA-MRSA has little impact on the pigs' health, it can be transmitted from pig to pig or from pig to human. This is a serious concern as people in direct contact with pigs are highly predisposed to acquiring LA-MRSA infection. The measures to control LA-MRSA spread in pig farms include conducting periodic LA-MRSA screening tests on pigs and avoiding certain antibiotics in pigs. This study aimed to review the emerging LA-MRSA strains in pig farms.

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Nasal staphylococci community of healthy pigs and pig-farmers in Aragon (Spain). Predominance and within-host resistome diversity in MRSA-CC398 and MSSA-CC9 lineages

et al. 2023

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Methicillin-resistant Staphylococcus aureus in swine housed indoors in Galicia, Spain

Cited by 3 publications

References 27 publications

Assessment of the Antibiotic Resistance Profile, Genetic Heterogeneity and Biofilm Production of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from The Italian Swine Production Chain

Assessment of the Antibiotic Resistance Profile, Genetic Heterogeneity and Biofilm Production of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from The Italian Swine Production Chain

A review of new emerging livestock-associated methicillin-resistant Staphylococcus aureus from pig farms

Nasal staphylococci community of healthy pigs and pig-farmers in Aragon (Spain). Predominance and within-host resistome diversity in MRSA-CC398 and MSSA-CC9 lineages

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