Methods for extraction, processing and detection of plant and soil nematodes.

Hooper, D. J.; Hallmann, Johannes; Subbotin, Sergei A.

doi:10.1079/9780851997278.0053

Cited by 304 publications

(224 citation statements)

References 0 publications

Supporting

Mentioning

205

Contrasting

Unclassified

Order By: Relevance

“…In order to observe RKN penetration, localization and subsequent development within the roots, fine root tips were stained with acid fuchsin, according to Byrd et al (1983) and Hooper et al (2005), then observed by stereomicroscopy (Leica MZ75) and bright-field light microscopy (Zeiss Axiophoto). Root segments were also embedded in resin for sectioning (4 lm).…”

Section: Histopathological Analysismentioning

confidence: 99%

Gene expression analysis inMusa acuminataduring compatible interactions withMeloidogyne incognita

Castañeda

Alves

Almeida

et al. 2017

Ann Bot

View full text Add to dashboard Cite

Background and Aims Endoparasitic root-knot nematodes (RKNs) (Meloidogyne spp.) cause considerable losses in banana (Musa spp.), with Meloidogyne incognita a predominant species in Cavendish sub-group bananas. This study investigates the root transcriptome in Musa acuminata genotypes 4297-06 (AA) and Cavendish Grande Naine (CAV; AAA) during early compatible interactions with M. incognita.Methods Roots were analysed by brightfield light microscopy over a 35 d period to examine nematode penetration and morphological cell transformation. RNA samples were extracted 3, 7 and 10 days after inoculation (DAI) with nematode J2 juveniles, and cDNA libraries were sequenced using lllumina HiSeq technology. Sequences were mapped to the M. acuminata ssp. malaccensis var. Pahang genome sequence, differentially expressed genes (DEGs) identified and transcript representation determined by gene set enrichment and pathway mapping.Key Results Microscopic analysis revealed a life cycle of M. incognita completing in 24 d in CAV and 27 d in 4279-06. Comparable numbers of DEGs were up-and downregulated in each genotype, with potential involvement of many in early host defence responses involving reactive oxygen species and jasmonate/ethylene signalling. DEGs revealed concomitant auxin metabolism and cell wall modification processes likely to be involved in giant cell formation. Notable transcripts related to host defence included those coding for leucine-rich repeat receptorlike serine/threonine-protein kinases, peroxidases, thaumatin-like pathogenesis-related proteins, and DREB, ERF, MYB, NAC and WRKY transcription factors. Transcripts related to giant cell development included indole acetic acid-amido synthetase GH3.8 genes, involved in auxin metabolism, as well as genes encoding expansins and hydrolases, involved in cell wall modification.Conclusions Expression analysis in M. acuminata during compatible interactions with RKNs provides insights into genes modulated during infection and giant cell formation. Increased understanding of both defence responses to limit parasitism during compatible interactions and effector-targeted host genes in this complex interaction will facilitate the development of genetic improvement measures for RKNs.

show abstract

Section: Histopathological Analysismentioning

confidence: 99%

Gene expression analysis inMusa acuminataduring compatible interactions withMeloidogyne incognita

Castañeda

Alves

Almeida

et al. 2017

Ann Bot

View full text Add to dashboard Cite

show abstract

“…Extracción de nematodos de suelo y raíces: la extracción de nematodos de suelo se realizó mediante el método de Baermann modificado (Hooper et al 2005). Se pesaron 100 g de suelo y se colocaron en un filtro de papel toalla que estaba extendido sobre un tamiz dentro de un recipiente.…”

Section: Análisis De Suelo Nematodos Y Datos Pluviométricosunclassified

“…La extracción de nematodos de raíces se realizó mediante el método de macerado y filtrado (Hooper et al 2005). Se pesaron 25 g de raíces y se colocaron en una licuadora con 200 ml de agua, seguidamente se licuó por 30 segundos.…”

Section: Análisis De Suelo Nematodos Y Datos Pluviométricosunclassified

Nematodos fitoparásitos asociados al tomate en la zona occidental de Nicaragua.

Antón

Guzmán-Hernández

2013

Agron. Mesoam.

View full text Add to dashboard Cite

“…To reach the planned Meloidogyne infestation, 20 g infested soil with pieces of galled roots were placed under the roots of tomato plants during planting. (For the Meloidogyne infestation not J2 nematodes (Hooper et al 2005) were used, because our opinion was that although the number of nematodes are known in that way, but the juveniles can get easily injured during the extraction process. Therefore they may lose their virulence.…”

Section: Interaction Experimentsmentioning

confidence: 99%

Effect of leaf litter mulching on the pests of tomato

Petrikovszki¹,

Körösi²,

Nagy³

et al. 2016

Columella

View full text Add to dashboard Cite

Agricultural techniques essentially change the weed flora, pests and diseases of cultivated plants. Our aim was to investigate the effect of leaf litter mulch on the pests of tomato, especially on the weed flora and on three important plant protection problems: late blight (Phytophthora infestans), cotton bollworm (Helicoverpa armigera) and root-knot nematodes (Meloidogyne spp.). Besides mulching, the experiment consisted of irrigation, mycorrhiza-inoculation and artificial Meloidogyne-infestation. We recorded the natural P. infestans infection, Helicoverpa armigera damage and the species composition and cover of weeds as well. We examined whether leaf litter mulching had any effect on pests, and on the generative and vegetative production of tomato. Our results were that only mulching had a significant positive effect on almost all the measured generative and vegetative production parameters of tomato, while irrigation, mycorrhiza application and artificial nematode infestation had no significant effects on these parameters. Negative correlation was found between P. infestans infection and H. armigera-damage on tomato fruits. Mulching also reduced significantly the number of Meloidogyne-induced galls on the roots of tomato. Application of leaf litter mulch did not affect soil organic matter and soil pH within the growing season. In the beginning of the growing season mulching suppressed weed cover. Later, there was no significant difference between weed cover in the treatments. It can be concluded that leaf litter mulch had a more pronounced influence on the quantity of yield than on the damage and presence of pests and weeds.

show abstract

Methods for extraction, processing and detection of plant and soil nematodes.

Cited by 304 publications

References 0 publications

Gene expression analysis inMusa acuminataduring compatible interactions withMeloidogyne incognita

Gene expression analysis inMusa acuminataduring compatible interactions withMeloidogyne incognita

Nematodos fitoparásitos asociados al tomate en la zona occidental de Nicaragua.

Effect of leaf litter mulching on the pests of tomato

Contact Info

Product

Resources

About