Methods for the detection and isolation of Escherichia coli including pathogenic strains

Hofstra, H.; Veld, J.H.J. Huisin't

doi:10.1111/j.1365-2672.1988.tb04471.x

Cited by 9 publications

(8 citation statements)

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“…Also, not only is E. coli of interest as a pathogen in clinical specimens, but there is a growing interest in the direct detection of pathogenic E. coli in food and in water samples. The development of rapid methods which are highly specific for identifying E. coli have been reviewed (Hofstra and Huis in 't Veld 1988;Wolcott 1991). In this review, stress has been placed on the usefulness of a variety of methods used in E. colz assays based on detecting B-glucuronidase (GUD) activity.…”

Section: Introductionmentioning

confidence: 99%

Methods for Escherichia coli identification in food, water and clinical samples based on beta‐glucuronidase detection

Frampton

Restaino

1993

Journal of Applied Bacteriology

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Section: Introductionmentioning

confidence: 99%

Methods for Escherichia coli identification in food, water and clinical samples based on beta‐glucuronidase detection

Frampton

Restaino

1993

Journal of Applied Bacteriology

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“…These false positives were not tested further because they were killed at the elevated temperature (44°C) in the indole test. They could either have been Bglucuronidase positive strains of Shigeila, Salmonella or Yersinia (Hofstra & Huis in't Veld 1988) or indole negative strains of E. coli. Less than 4% of non-fluorescent or non-chromogenic isolates proved to be E. coli after identification, and in the preferred medium (BCIG at a concentration of 125mg/l) this value dropped to < 2%.…”

Section: Resultsmentioning

confidence: 99%

“…A characteristic of 9 6 9 7 % of E. coli (Damare et al 1985) is the production of p-D-glucuronidase @-glut). Only a few other enterics (some strains of Shigella, Salmonella and Yersinia) have this enzyme (Hofstra & Huis in't Veld 1988) and as these are human pathogens, their detection by this method is acceptable. The activity of 8-gluc can be determined by the addition of suitable chromogenic or fluorogenic substrates to the medium.…”

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confidence: 99%

An evaluation of fluorogenic and chromogenic assays for the direct enumeration of Escherichia coli

Ogden¹,

Watt²

2008

Letters in Applied Microbiology

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Three direct plating methods to enumerate Escherichia coli from food in 24 h are described. Unlike the majority of enterics, 96% of E. coli are able to cleave β‐glucuronic acid. This reaction can be observed by incorporating (1) 4‐methylumbelliferyl‐β‐D‐glucuronate, (2) para‐nitrophenyl‐β‐D‐glucuronate or (3) 5‐bromo‐4‐chloro‐3‐indolyl‐β‐D‐glucuronate (BCIG) into a peptone tergitol agar base. Escherichia coli produce fluorescent, yellow or deep blue colonies from these three compounds respectively. BCIG agar proved the easiest to read and produced the least number of false positives and false negatives. An attempt is made to explain the poor correlation achieved between this method and a conventional most probable number technique.

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“…Os coliformes termotolerantes estão associados a um elevado número de patologias cujos agentes etiológicos são isolados em laboratórios de microbiologia clínica e diretamente considerados o motivo da maioria das infecções intestinais humanas conhecidas. O indicador patogênico de origem fecal mais importante é a Escherichia coli, micro-organismo designado como termotolerante, desprovido de vida livre no ambiente, indicando que quando presente na água, a mesma está contaminada por fezes 26 . Além de infecções intestinais, os coliformes podem estar envolvidos ou ter participação em diversas outras patologias, como meningites, intoxicações alimentares, infecções urinárias e pneumonias, inclusive as nosocomiais.…”

Section: Quadro 1 Organismos Patogênicos De Veiculação Hídrica (Contunclassified

Qualidade microbiológica da água para consumo humano em instituição de ensino de Maringá-PR

Yamaguchi¹,

Cortez²,

Ottoni³

et al. 2013

Mundo Saúde

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ResumoA água contaminada associada à falta de saneamento básico mata anualmente cerca de 1,6 milhões de pessoas no mundo.A falta da potabilidade da água nem sempre é perceptível à visão ou olfato, sendo necessária uma análise laboratorial para detectá-la. Torna-se importante o controle microbiológico da água devido sua característica de veículo de transmissão de bactérias, dentre estas, coliformes totais e termotolerantes, protozoários, vírus e fungos causadores de inúmeras doenças ao homem. Esses micro-organismos são responsáveis pela ocorrência de diarreias, disenterias, hepatites, cólera, entre outras enfermidades graves. Esta pesquisa teve por finalidade analisar a presença de coliformes totais e termotolerantes em amostras de água mineral engarrafada e água tratada. No presente estudo foram selecionados 50 locais para coleta de água para consumo humano, todos localizados em uma instituição de ensino na cidade de Maringá-PR. Das amostras coletadas, 13 foram provenientes de bebedouros de água mineral natural engarrafada e 37 amostras de bebedouros de pressão de água tratada na própria instituição de ensino. A metodologia empregada para pesquisa de coliformes foi o método de fermentação em tubos múltiplos. O resultado deste trabalho revelou, por meio de análise microbiológica, que as amostras de água tratada dos bebedouros de pressão apresentaram-se próprias para consumo humano. Entretanto, 15,38% das amostras de água mineral apresentaram-se contaminadas por coliformes totais. Esses resultados demonstraram que a água tratada com cloro apresentou melhor qualidade microbiológica quando comparada à água mineral natural engarrafada.Palavras-chave: Aqua Petra. Água Potável. Análise de Água. Cloro. Coliformes. AbstractContaminated water associated with poor sanitation kills about 1.6 million people worldwide annually. Lack of potability in water is not always perceptible to the sight or smell, requiring laboratory analysis to detect it. Because of that it is important to control microbiologically water since it is a vehicle of transmission of bacteria such as total and fecal coliforms, protozoa, viruses and fungi that cause numerous humans diseases. These microorganisms cause diarrhea, dysentery, hepatitis, cholera, among other serious illnesses. This research aimed at examining the presence of total and fecal coliforms in samples from bottled mineral water and treated water. In the present study 50 sites of drinking water were selected, all located in an educational institution in Maringá-PR. 13 of the collected samples were from water drinker dispensers with bottled natural mineral water and 37 samples were from water dispensers with water treated in their own institution. The methodology used for detecting coliforms was the method of Multiple Tube Fermentation. The result of this study revealed -through microbiological analysis -that the samples of treated water were fit for human consumption. However, 15.38% of the analyzed mineral water samples were contaminated with total coliforms. These results showed that w...

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Methods for the detection and isolation of Escherichia coli including pathogenic strains

Cited by 9 publications

References 54 publications

Methods for Escherichia coli identification in food, water and clinical samples based on beta‐glucuronidase detection

Methods for Escherichia coli identification in food, water and clinical samples based on beta‐glucuronidase detection

An evaluation of fluorogenic and chromogenic assays for the direct enumeration of Escherichia coli

Qualidade microbiológica da água para consumo humano em instituição de ensino de Maringá-PR

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