Methyl jasmonate-induced biosynthesis of taxol and expression of certain related genes by Hazelnut (Corylus avellana L.) cells

Rezaei, Abdul Rahim; Ghanati, Faezeh; Behmanesh, Maryam

doi:10.1055/s-0031-1282607

Cited by 3 publications

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“…MJ and SA have been considered for inducing terpene biosynthesis instead of other treatments like mechanical wounding, which has been used in several studies to induce secondary metabolism in different plants (Chen and Chen 1999;Ishihara et al 2002;Wang et al 2004;Rezaei et al 2011). MJ and SA do not cause physical damage on terpene storage sites and do not destroy the tissues/organs involved in terpene biosynthesis/accumulation, and hence could be a good tool for parthenolide increase, as it has been shown that parthenolide biosynthesis occurs in glandular trichomes which are distributed on the surface of aerial tissues of T. parthenium including stem, leaves and flowers (Majdi et al 2011).…”

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“…MJ and SA do not cause physical damage on terpene storage sites and do not destroy the tissues/organs involved in terpene biosynthesis/accumulation, and hence could be a good tool for parthenolide increase, as it has been shown that parthenolide biosynthesis occurs in glandular trichomes which are distributed on the surface of aerial tissues of T. parthenium including stem, leaves and flowers (Majdi et al 2011). MJ and SA have been successfully used to enhance secondary metabolite production in intact plants or plant cell cultures of different plant species, e.g, alkaloids, terpenoids, phenolic phytoalexins, coumarins and taxanes (Bulgakov et al 2002;Kim et al 2006;Wang et al 2010;Rezaei et al 2011;Peng et al 2011;Perez et al 1997;Fang et al 1999). Although it has been reported that MJ and SA influence sesquiterpene lactone biosynthesis, e.g., crepidiaside B, 8-deoxylactucin and the germacranolide sonchuside A in hairy roots of chicory (Cichorium intybus) (Malarz et al 2007), their effects on parthenolide content are still unclear.…”

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Parthenolide accumulation and expression of genes related to parthenolide biosynthesis affected by exogenous application of methyl jasmonate and salicylic acid in Tanacetum parthenium

2015

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Up-regulation of germacrene A synthase and down-regulation of parthenolide hydroxylase genes play key role in parthenolide accumulation of feverfew plants treated with methyl jasmonate and salicylic acid. Parthenolide is an important sesquiterpene lactone due to its anti-migraine and anti-cancer properties. Parthenolide amount was quantified by high-performance liquid chromatography after foliar application of methyl jasmonate (100 µM) or salicylic acid (1.0 mM) on feverfew leaves in time course experiment (3-96 h). Results indicate that exogenous application of methyl jasmonate or salicylic acid activated parthenolide biosynthesis. Parthenolide content reached its highest amount at 24 h after methyl jasmonate or salicylic acid treatments, which were 3.1- and 1.96-fold higher than control plants, respectively. Parthenolide transiently increased due to methyl jasmonate or salicylic acid treatments until 24 h, but did not show significant difference compared with control plants at 48 and 96 h time points in both treatments. Also, the transcript levels of early pathway (upstream) genes of terpene biosynthesis including 3-hydroxy-3-methylglutaryl-coenzyme A reductase, 1-deoxy-D-xylulose-5-phosphate reductoisomerase and hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase and the biosynthetic genes of parthenolide including germacrene A synthase, germacrene A oxidase, costunolide synthase and parthenolide synthase were increased by methyl jasmonate and salicylic acid treatments, but with different intensity. The transcriptional levels of these genes were higher in methyl jasmonate-treated plants than salicylic acid-treated plants. Parthenolide content measurements along with expression pattern analysis of the aforementioned genes and parthenolide hydroxylase as side branch gene of parthenolide suggest that the expression patterns of early pathway genes were not directly consistent with parthenolide accumulation pattern; hence, parthenolide accumulation is probably further modulated by the expression of its biosynthetic genes, especially germacrene A synthase and also its side branch gene, parthenolide hydroxylase.

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Parthenolide accumulation and expression of genes related to parthenolide biosynthesis affected by exogenous application of methyl jasmonate and salicylic acid in Tanacetum parthenium

2015

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“…Taxol extraction from powdered dried cells was based on those previously described with some modifications . In brief, the dried cells were pulverized and suspended in 10 mL methanol, ultrasonicated for 40 min and then filtered.…”

Section: Methodsmentioning

confidence: 99%

Paclitaxel production is enhanced in suspension‐cultured hazel (Corylus avellana L.) cells by using a combination of sugar, precursor, and elicitor

Rahpeyma

Moieni

Javaran

2015

Engineering in Life Sciences

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Hazel (Corylus avellana L.) has recently been drawing attention as an alternative source of taxol. In the present study, the effects of sugar type, and different concentrations of phenylalanine (Phe) and vanadyl sulfate (V) on the production of taxol in C. avellana were investigated. A factorial experiment was used to optimize the concentrations of the precursor and elicitor. The cells were treated with Phe and V on the fourth day of culture and were harvested every 2 days until the 10th day. By increasing the Phe and V supply, taxol production increased during the culture period and the maximum level of 4.2 μg/g (dry weight) was obtained at day 10 by combining 3 μM of Phe and 0.05 and 0.1 mM of V in media supplemented with fructose (3%). The time course study on taxol production suggested that the appropriate time for using Phe is day 4 of culture, and day 8 for V. Overall, taxol production in C. avellana cell suspension culture was improved by the use of the combined strategy.

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Changes in Physiological Traits, Gene Expression and Phytochemical Profile of Mentha piperita in Response to Elicitor

Afkar,

Karimzadeh

2024

Biochem Genet

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Methyl jasmonate-induced biosynthesis of taxol and expression of certain related genes by Hazelnut (Corylus avellana L.) cells

Cited by 3 publications

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Parthenolide accumulation and expression of genes related to parthenolide biosynthesis affected by exogenous application of methyl jasmonate and salicylic acid in Tanacetum parthenium

Parthenolide accumulation and expression of genes related to parthenolide biosynthesis affected by exogenous application of methyl jasmonate and salicylic acid in Tanacetum parthenium

Paclitaxel production is enhanced in suspension‐cultured hazel (Corylus avellana L.) cells by using a combination of sugar, precursor, and elicitor

Changes in Physiological Traits, Gene Expression and Phytochemical Profile of Mentha piperita in Response to Elicitor

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