Methyl methane sulphonate induced desynaptic mutants in Capsicum annuum L.

Gulfishan, Mohd; Jafri, Iram Fatima; Khan, Arfaat; Bhat, Tariq Ahmad

doi:10.3199/iscb.8.59

Cited by 11 publications

(5 citation statements)

References 18 publications

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“…A better alternative term for desynapsis is proposed as synaptic mutant by Riley and Law (1965). Male meiotic mutant like desynapsis is reported both from natural (vide Koduru and Rao 1981, Poddar et al 1998, Armstrong and Jones 2003, Jackson et al 2002, Sosnokhina et al 2002, Maity and Datta 2009, Mandal and Datta 2011 and mutagen induced (Bozzini and Martini 1971, Gottschalk and Baquar 1971, Palmer 1974, Gottschalk and Kaul 1980, Datta and Biswas 1985, Bhattacharya and Datta 2012, Gulfishan et al 2013, Naseem and Kumar 2013, Bhat and Wani 2015 population of different plant species. Apart from cytogenetical significance, desynaptic mutants can potentially induce aneuploids (Soost 1951, Burnham 1962, Jackson et al 2002.…”

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confidence: 99%

Copper Oxide Nanoparticles Induced Fertile Desynaptic Mutant Line in Coriandrum sativum L. (Apiaceae)

et al. 2018

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A desynaptic plant (2n=22) is screened from copper oxide nanoparticles (CuO-NPs) treated (dry seeds exposed to 0.25 µg mL 1 , 4 h) M 1 population (one from 47 plants) of Coriandrum sativum L. (moisture content: 13.60%). Selfed seeds of the marked plant also yield a desynaptic plant (one plant out of five raised; 50 seeds sown) at M 2 . The mutant plants show enhanced univalent frequency (diplotene-diakinesis: 0-14/cell, metaphase I (MI): 0-20/cell) and reduced chiasma/cell (diplotene-diakinesis: 12.58 0.95 to 12.20 1.34; MI: 12.11 1.13 to 11.64 0.97) than dry and bulk controls (diplotene-diakinesis: univalent frequency 0-4/cell, chiasma/cell 14.77 1.20 to 13.00 0.92; MI 0-4/cell, chiasma/cell 13.85 0.93 to 12.91 0.49). The marked plants document unequal chromosome segregation at anaphase I (AI) (10/12, 9/13, 8/14 and 6/16) and anaphase II (AII) (unequal groups) compared to cytologically balanced separation in controls. Pollen grains fertility is also reduced in the mutant plants (83.36 to 77.76%) than controls (96.91 to 89.88%). Both the M 1 and M 2 desynaptic plants yield seeds. Results suggest that CuO-NPs can alter meiotic synchrony of genes controlling retention of chiasma formation and maintenance, leading to the development of desynaptic mutant line.

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Copper Oxide Nanoparticles Induced Fertile Desynaptic Mutant Line in Coriandrum sativum L. (Apiaceae)

et al. 2018

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“…Previously it has been reported that the presence of univalents at metaphase I might be due to asynapsis (lack of chromosome pairing during the late prophase I), so that the homologous chromosomes failed to pair (Bione et al 2002) or desynapsis (inability to retain chiasmata in synapsed homologous chromosomes) resulting in premature separation of bivalents (Kumar et al 2006, Gulfishan et al 2013. The multivalent formation was due to the breakage in chromosomes caused by mutagens and their reunion through reciprocal translocations (Khan and Verma 2017).…”

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confidence: 99%

Isolation of mutants from gamma ray-irradiated population of Solanum melongena cultivar ‘Pusa Purple Long’

et al. 2018

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Three mutants isolated according to differed morphological traits were isolated from the M 2 progeny of gamma ray-irradiated Solanum melongena L. var. PPL, viz. PPL-j 3 (5kR), PPL-k 3-1 (15 kR), and PPL-k 3-2 (15 kR). The seeds of selected mutants of M 2 generation were collected individually and grown in M 3 generation, separately and data on various quantitative traits, such as plant height (cm), number of branches per plant, number of fruits per plant, fruit weight (g), fruit size (cm), 1000-seed weight (g), and yield (kg) per plant, were recorded for all three mutants. The mutants were morphologically quite distinct, as compared to the control and to each other. The average performance of different quantitative traits was better in a high yielding mutant (PPL-j 3) as compared to the control, while in other two mutants (PPL-k 3-1 and PPL-k 3-2), poor yield was observed. Cytological analysis revealed almost normal chromosomal set in mutant screened from 5kR irradiated population, except for a few chromosomal aberrations. The higher frequency of meiotic aberrations was recorded in mutants isolated from 15 kR irradiated population. However, the frequency of these chromosomal irregularities decreased at anaphase, exhibiting recovery at later stages. A reduction in pollen fertility was also recorded in mutants isolated from 15 kR, indicating low fruit set.

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“…Desynapsis has been found to reduce male fertility in plants. The sterility encountered in the desynaptics could be associated with the homozygous condition of a single recessive gene (Gulfishan et al 2013). The pleiotropic effects of the mutant gene(s) like breakage, stickiness and spindle abnormalities, will also contribute to pollen sterility (Thomas and Rajhathy 1966).…”

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confidence: 99%

Cytogenetical Study of Induced Desynaptic Variants in Phaseolus vulgaris L.

Chaudhary

Kumar

2017

CYTOLOGIA

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The cytological screening of gamma ray treated plants revealed few desynaptic plants where an enhanced frequency of univalents were found at metaphase I along with few bivalents. The desynaptic plants were obtained at 200 Gy dose of gamma rays and were characterised as weak and medium strong type. The univalents remained unpaired till later meiotic stages and resulted in the unequal separation of chromosomes at anaphase I. The unequal separation of chromosomes as a consequence of desynapsis lead to the formation of micronuclei and gametes with imbalanced chromosomes, thus affecting the post-meiotic stages also. The desynaptic plants possess very few pods and acquire high pollen inviability. The study suggests the ability of gamma rays in the creation of male sterile lines in Phaseolus vulgaris L. The gamma rays either act on the genes responsible for synapsis and chiasma formation or disrupt the synaptonemal complex affecting chiasmate dissociation. The desynaptic mutants could be used as a potential source for gathering information on chiasma maintenance mechanism as well as to study the genetic mechanism and consequences of male sterility in plants. Further, the male sterile lines could also be profitably utilized in hybridization breeding programmes to produce hybrid seeds.

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Methyl methane sulphonate induced desynaptic mutants in Capsicum annuum L.

Cited by 11 publications

References 18 publications

Copper Oxide Nanoparticles Induced Fertile Desynaptic Mutant Line in <i>Coriandrum sativum</i> L. (Apiaceae)

Copper Oxide Nanoparticles Induced Fertile Desynaptic Mutant Line in <i>Coriandrum sativum</i> L. (Apiaceae)

Isolation of mutants from gamma ray-irradiated population of <i>Solanum melongena</i> cultivar ‘Pusa Purple Long’

Cytogenetical Study of Induced Desynaptic Variants in <i>Phaseolus vulgaris</i> L.

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