Methyltransferase like 13 mediates the translation of Snail in head and neck squamous cell carcinoma

Wang, Xiaochen; Li, Kang; Wan, Yuehan; Chen, Fangfang; Cheng, Maosheng; Xiong, Guanglei; Wang, Ganping; Chen, Shuang; Chen, Zhi; Chen, Jianwen; Xu, Xiuyun; Wang, Cheng; Peng, Liang; Chen, Demeng

doi:10.1038/s41368-021-00130-8

Cited by 14 publications

(18 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…20 Previously, METTL13 expression was markedly raised, and an absence of METTL13 greatly inhibited proliferation at the same time as it potentiated apoptosis in vitro, and hindered tumor growth in vivo in head and neck squamous cell carcinoma. 8 In addition, METTL13 overexpression drove cell growth and migration in gastric cancer. 10 Consistently, we also noted an abnormal upregulation of METTL13 in NPC cells.…”

Section: Discussionmentioning

confidence: 99%

“…METTL13, belonging to the N‐terminal protein methyltransferase family, has been supported to be related to diversified biological events and cellular processes in cancer progression 20 . Previously, METTL13 expression was markedly raised, and an absence of METTL13 greatly inhibited proliferation at the same time as it potentiated apoptosis in vitro, and hindered tumor growth in vivo in head and neck squamous cell carcinoma 8 . In addition, METTL13 overexpression drove cell growth and migration in gastric cancer 10 .…”

Section: Discussionmentioning

confidence: 99%

“…7 Recent studies revealed that METTL13 was dysregulated in several types of cancer and influenced tumorigenesis and progression. An upregulated level of METTL13 was discovered not only to be negatively associated with clinical prognosis, but also to drive cancer cell malignant phenotypes and facilitate cancer development and metastasis, functioning as an oncogenic role [8][9][10] ; on the contrary, METTL13 was reported to be lowly expressed and served as an anti-oncogene in bladder carcinoma and clear cell renal cell cancer. Elevated METTL13 expression was related to the favorable prognosis and was beneficial to inhibit tumor growth and metastasis.…”

mentioning

confidence: 99%

See 2 more Smart Citations

METTL13 promotes nasopharyngeal carcinoma progression through regulating the ZEB1/TPT1 axis

Liang

Zhou

et al. 2023

The Journal of Gene Medicine

View full text Add to dashboard Cite

Background Globally, nasopharyngeal carcinoma (NPC) is a prevalent and deadly malignancy. Despite the role of methyltransferase like 13 (METTL13) having been highlighted in a majority of human cancers, its function and mechanism in NPC is indistinct. Methods The expression level of METTL13 in NPC cell lines and normal cells was detected using a quantitative real‐time polymerase chain reaction. Gain‐ and loss‐of function experiments were conducted. Cell counting kit‐8, 5‐ethynyl‐2′‐deoxyuridine, wound‐healing, Transwell and tube formation assays, respectively, appraised the proliferative, migratory, invasive and angiogenic cellular responses. Corresponding protein expression was measured by western blotting. A chromatin immunoprecipitation assay was applied to verify the association between ZEB1 and the TPT1 promoter. Eventually, to substantiate the critical role of METTL13 in NPC, the establishment of an in vivo tumorigenesis model was accomplished. Results METTL13 possessed fortified expression in NPC cells. METTL13 silencing markedly suppressed NPC cellular phenotypes in vitro, including proliferative, migratory, invasive and angiogenic events, as well as hindered tumorigenesis in vivo. Additionally, METTL13 positively regulated ZEB1, whereas ZEB1 could bind to TPT1 promoter and transcriptionally regulate TPT1. TPT1 was also found to be upregulated in NPC cells. TPT1 silencing suppressed NPC cellular phenotypes in vitro. TPT1 overexpression partly weakened the anti‐tumor effect of METTL13 in NPC. Conclusions In summary, METTL13 up‐regulated ZEB1, which facilitated the transcriptional activation of TPT1, ultimately promoting NPC growth and metastasis, providing a potential therapeutic strategy for NPC treatment.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

METTL13 promotes nasopharyngeal carcinoma progression through regulating the ZEB1/TPT1 axis

Liang

Zhou

et al. 2023

The Journal of Gene Medicine

View full text Add to dashboard Cite

show abstract

“…In contrast, METTL13 levels have been reported as elevated and linked to unfavorable prognosis for other cancer types. In head and neck squamous cell carcinoma, METTL13 expression is increased at both the transcript and protein level and high levels are associated with poor prognosis [62]. In hepatocellular carcinoma, METTL13 has been implied in mediating tumor growth and metastasis [41].…”

Section: Connection To Diseasesmentioning

confidence: 99%

Structure, Activity and Function of the Dual Protein Lysine and Protein N-Terminal Methyltransferase METTL13

Jakobsson

2021

Life

View full text Add to dashboard Cite

METTL13 (also known as eEF1A-KNMT and FEAT) is a dual methyltransferase reported to target the N-terminus and Lys55 in the eukaryotic translation elongation factor 1 alpha (eEF1A). METTL13-mediated methylation of eEF1A has functional consequences related to translation dynamics and include altered rate of global protein synthesis and translation of specific codons. Aberrant regulation of METTL13 has been linked to several types of cancer but the precise mechanisms are not yet fully understood. In this article, the current literature related to the structure, activity, and function of METTL13 is systematically reviewed and put into context. The links between METTL13 and diseases, mainly different types of cancer, are also summarized. Finally, key challenges and opportunities for METTL13 research are pinpointed in a prospective outlook.

show abstract

“…As an illustration, both in vitro and in vivo tumor growth is inhibited by silencing of the m6A demethylase alkB homolog 5 RNA demethylase (ALKBH5) [ 17 , 18 ]. By increasing snail family transcriptional repressor 1 (SNAIL1) mRNA translation, the protein methyltransferase methyltransferase-like 13 (METTL13) promotes OSCC development and maintenance [ 19 ]. Therefore, it is necessary to elucidate the potential mechanism of oncogenesis and progression of OSCC.…”

Section: Introductionmentioning

confidence: 99%

TAB2 Promotes the Biological Functions of Head and Neck Squamous Cell Carcinoma Cells via EMT and PI3K Pathway

et al. 2022

View full text Add to dashboard Cite

Background. Transforming growth factor β1-activated kinase 1 binding protein 2 (TAB2) mediates a variety of biological processes through activated nuclear factor κ-light-chain-enhancer of activated B cell (NF-κB) signaling pathways. TAB2 has been reported to be upregulated in a variety of tumors. However, little is known about its potential role in oral squamous cell carcinoma (OSCC). Material and Methods. Patients’ clinicopathological and transcription data were obtained from The Cancer Genome Atlas (TCGA) database. Immunohistochemistry staining was used to determine TAB2 expression in OSCC tissues (IHC). The expression of TAB2 in OSCC cell lines was detected by western blotting. The CCK-8 test and flow cytometry assay were utilized to evaluate cell proliferation, apoptosis, and cell cycle in OSCC cell lines. Enrichment analysis and identification of predicted signaling pathways were performed by Gene Ontology and KEGG analysis. Finally, the expression of downstream signal molecules was performed using western blotting to validate the mechanism investigations. Results. TAB2 expression level was aberrantly upregulated in OSCC patients. TAB2 expression was shown to be inversely associated to prognosis. The phenotypic of OSCC cells was considerably impacted by TAB2. OSCC cells with deleted TAB2 exhibit decreased proliferation and increased apoptosis. Additionally, OSCC progression is aided by TAB2 overexpression. Further mechanism studies showed that TAB2 could regulate the progression of OSCC by mediating the upregulation of EMT and PI3K-AKT signaling pathways. Conclusion. This study sheds light on the carcinogenic role of TAB2 in OSCC and provides a potential therapeutic strategy.

show abstract

Methyltransferase like 13 mediates the translation of Snail in head and neck squamous cell carcinoma

Cited by 14 publications

References 40 publications

METTL13 promotes nasopharyngeal carcinoma progression through regulating the ZEB1/TPT1 axis

METTL13 promotes nasopharyngeal carcinoma progression through regulating the ZEB1/TPT1 axis

Structure, Activity and Function of the Dual Protein Lysine and Protein N-Terminal Methyltransferase METTL13

TAB2 Promotes the Biological Functions of Head and Neck Squamous Cell Carcinoma Cells via EMT and PI3K Pathway

Contact Info

Product

Resources

About