MgMfs1, a major facilitator superfamily transporter from the fungal wheat pathogen Mycosphaerella graminicola, is a strong protectant against natural toxic compounds and fungicides

Roohparvar, Ramin; Waard, M.A. de; Kema, G.H.J.; Zwiers, L.H.

doi:10.1016/j.fgb.2006.09.007

Cited by 95 publications

(89 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Moreover, transporters may prevent suicidal effects by releasing patulin in the surrounding tissues or in the growth medium. For instance, TOXA gene in Cochliobolus carbonum encodes an efflux pump which contributes to self-protection against its own toxin and/or is involved in toxin secretion (Roohparvar et al 2007). The modification of a self-defence mechanism in P. expansum could be involved in the mode of action of quercetin and umbelliferone since unreleased patulin might interfere with the normal secondary fungal metabolism.…”

Section: Discussionmentioning

confidence: 99%

Effect of quercetin and umbelliferone on the transcript level of Penicillium expansum genes involved in patulin biosynthesis

et al. 2009

View full text Add to dashboard Cite

Penicillium expansum is commonly associated with patulin accumulation in pome fruits. In in vitro studies, two phenolic compounds (quercetin and umbelliferone) proved to be effective in reducing patulin accumulation, particularly when applied in combination, without consistently affecting mycelial growth. To investigate the mode of action of quercetin and umbelliferone, the expression of five genes likely involved in patulin biosynthesis was evaluated using real-time PCR in the presence and absence of the tested phenolic compounds. The relative expression of genes coding isoepoxydon dehydrogenase (IDH), 6-methylsalicylic acid synthase (msas) and an ATP-binding cassette transporter (peab1) proved to be down-regulated when quercetin and umbelliferone were added in combination. Furthermore, the relative expression of two putative cytochrome P450 monooxygenases (p450-1 and p450-2) was reduced by all treatments, although the combination of the two substances was the most effective. These results provide evidence that quercetin and umbelliferone reduce patulin accumulation by acting on the transcription level of the tested genes

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of quercetin and umbelliferone on the transcript level of Penicillium expansum genes involved in patulin biosynthesis

et al. 2009

View full text Add to dashboard Cite

show abstract

“…Hence, it might be that reversal of the activity of one or more of these ABC transporters in M. graminicola by the phenothiazines or other compounds tested is responsible for the synergism with cyproconazole. The MFS transporter MgMfs1 has also been described as a potent transporter of azole fungicides (27). However, the compounds tested are not described in the literature as modulators of MFS transporters, and therefore modulation of MgMfs1 by phenothiazines is probably not responsible for the synergism observed.…”

Section: Discussionmentioning

confidence: 99%

“…However, the plant defense compounds acting as substrates for these transporters have not yet been identified (32,34). MFS transporters can also mediate natural insensitivity to plant defense compounds, as shown for Bcmfs1 from B. cinerea and MgMfs1 from M. graminicola (15,27).…”

mentioning

confidence: 99%

Control of Mycosphaerella graminicola on Wheat Seedlings by Medical Drugs Known To Modulate the Activity of ATP-Binding Cassette Transporters

Roohparvar

Huser

Zwiers

et al. 2007

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

Medical drugs known to modulate the activity of human ATP-binding cassette (ABC) transporter proteins (modulators) were tested for the ability to potentiate the activity of the azole fungicide cyproconazole against in vitro growth of Mycosphaerella graminicola and to control disease development due to this pathogen on wheat seedlings. In vitro modulation of cyproconazole activity could be demonstrated in paper disk bioassays. Some of the active modulators (amitriptyline, flavanone, and phenothiazines) increased the accumulation of cyproconazole in M. graminicola, suggesting that they reversed cyproconazole efflux. However, synergism between cyproconazole and modulators against M. graminicola on wheat seedlings could not be shown. Despite their low in vitro toxicity to M. graminicola, some modulators (amitriptyline, loperamide, and promazine) did show significant intrinsic disease control activity in preventive and curative foliar spray tests with wheat seedlings. The results suggest that these compounds have indirect disease control activity based on modulation of fungal ABC transporters essential for virulence and constitute a new class of disease control agents.

show abstract

“…Further studies showed that this protein leading to the disease of soybean,,which involved in the secretion of cercosporin from C. kikuchii to soybean, nevertheless, mutant △CFP reducing the toxicity to soybean with no cercosporin. MgMfs1, a gene of major facilitator superfamily, was identified from the wheat pathogen fungal Mycosphaerella graminicola, the encoded protein contain 14 transmembrane spannersand which is high homology to the MFS drug: H + transporters [23], It is suggested that this protein particaipated in the secretion of cercosporin and the inhibition of various drugs.A gene named tep1was found by Pieter, which shows high homology to the members of MFS transporters and encodes a transmemberane protein , conferring chlotamphenicol resistance in Sinorhizobium meliloti by expelling the antibiotic outside the bacteria [24]. The identification of tep1 mutant found there is no feedback regulation of nodulation in S. meliloti, but the accumulation of the Nod factor precursor N-acetyl glucosamine in S. meliloti inhibits the expression of nod gene and reduces the nodule formation efficiency, It is suggested that Tep1 could transport the high concentration of N-acetyl glucosamine in S. meliloti and lead to a reduction of nod gene expression in alfalfa plants.…”

Section: A Mfs Multidrug Transporters and Pathogensmentioning

confidence: 99%

Roles of Multidrug Transporters of MFS in Plant Stress Responses

Hua¹,

Su-fen²,

Luo³

et al. 2011

IJBBB

View full text Add to dashboard Cite

Abstract-Multidrug transporters are a class of secondary transporters and important transmembrane transporters which belong to facilitator superfamily (MFS). Multidrug transporters play critical roles in plant defense against various toxin stresses by exporting toxin outside the cell to reduce the accumulation of toxin compounds in body. In this article, structural features and mechanisms of MFS multidrug transporters responsed to plant stresses are described in details , such as defending against the infection of pathogen, exporting toxic compounds and transporting the heavy metal and so on. And then, the molecular modulation mechanism of plants MFS multidrug transporters is preliminarily discussed and the important roles of these transporters are defineds responsed to sresses in plants.

show abstract

MgMfs1, a major facilitator superfamily transporter from the fungal wheat pathogen Mycosphaerella graminicola, is a strong protectant against natural toxic compounds and fungicides

Cited by 95 publications

References 38 publications

Effect of quercetin and umbelliferone on the transcript level of Penicillium expansum genes involved in patulin biosynthesis

Effect of quercetin and umbelliferone on the transcript level of Penicillium expansum genes involved in patulin biosynthesis

Control of Mycosphaerella graminicola on Wheat Seedlings by Medical Drugs Known To Modulate the Activity of ATP-Binding Cassette Transporters

Roles of Multidrug Transporters of MFS in Plant Stress Responses

Contact Info

Product

Resources

About