Micellar electrokinetic chromatography with polyelectrolyte complexes as micellar pseudo-stationary phases

Shpak, Alexey V.; Пирогов, А. В.; Шпигун, О. А.

doi:10.1016/j.jchromb.2003.10.072

Cited by 13 publications

(17 citation statements)

References 24 publications

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“…Similar studies used complexes of polyacrylic acid and polymethacrylic acid with the cationic surfactant dodecyltrimethylammonium bromide [27,28]. Contrary to conventional wisdom, no reversal in electroosmotic flow was observed, and the mixed anionic/cationic structures maintained sufficient mobility to provide broad migration windows.…”

Section: Acrylate Copolymersmentioning

confidence: 98%

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Recent progress in the use of soluble ionic polymers as pseudostationary phases for electrokinetic chromatography

Palmer

McCarney

2004

Electrophoresis

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This review concerns the development, characterization, and application of soluble ionic polymeric materials as pseudostationary phases for electrokinetic chromatography since 2002. Cationic polymers, anionic siloxanes, polymerized surfactants (micelle polymers), and chiral polymers are considered. The use of stable suspensions of polymer nanoparticles in electrokinetic chromatography is also reviewed.

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Section: Acrylate Copolymersmentioning

confidence: 98%

“…Separation of dansyl amino acids using poly (acrylic acid) dodecyl trimethyl ammonium bromide complex in 50 mM phosphate buffer, pH 6.0. Reprinted from [28], with permission.…”

Section: Acrylate Copolymersmentioning

confidence: 99%

Recent progress in the use of soluble ionic polymers as pseudostationary phases for electrokinetic chromatography

Palmer

McCarney

2004

Electrophoresis

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“…PSPs composed of complexes of polyacrylic acid and polymethacrylic acid with the cationic surfactant dodecyltrimethylammonium bromide were introduced [45], and the selectivity of the complexes was studied as a function of complex composition [46,47]. The addition of the cationic surfactant did not reverse the EOF, and the complexes had sufficient mobility to provide broad migration windows.…”

Section: Structural Studiesmentioning

confidence: 99%

Electrokinetic chromatography with polymeric pseudostationary phases

Palmer¹

2008

J of Separation Science

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Recent research and development efforts concerning polymeric pseudostationary phases (PSPs) for electrokinetic chromatography are reviewed. The introduction of new materials, characterization of structural effects on performance and selectivity, applications, and the use of polymeric PSPs with mass spectrometric detection are considered. Very interesting results concerning the effects of polymer structure have been reported. Significant developments have also been reported in the development of novel applications of polymeric PSPs, particularly for sample preconcentration using micellar affinity gradient focusing. The use of mass spectrometric detection with electrokinetic chromatography has seen significant development, and recent reports indicate that this is a robust and sensitive approach.

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“…The cause of aggregate formation is usually attributed to the cooperative binding between surfactants and polyelectrolytes [27,29], depending on hydrophobic and electrostatic interactions. During recent years, polyelectrolyte complexes have been used as novel pseudo-stationary phases in MEKC [32][33][34]. Sabate et al have studied the electrophoretic properties of DTAB by using Doppler microelectrophoresis [35].…”

Section: Principle Of Dtab Carrying Powermentioning

confidence: 99%

On-line sample preconcentration by sweeping with dodecyltrimethylammonium bromide in capillary zone electrophoresis

Gong

Wehmeyer

Limbach

et al. 2006

Journal of Chromatography A

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On-line sample preconcentration of oligonucleotides with a new sweeping carrier was developed by using dodecyltrimethylammonium bromide (DTAB) below the critical micelle concentration (CMC). The sweeping results with DTAB below and above the CMC were compared. The use of DTAB below the CMC benefits the preconcentration of the oligonucleotides, while the use of DTAB above the CMC is good for hydrophobic small molecules. The factors affecting the sweeping results were optimized and this method was evaluated by constructing calibration curves for thrombin aptamers. The sweeping scheme produced a 112-fold sensitivity enhancement for the oligonucleotides relative to that run in a running buffer without DTAB. The sweeping method developed here can be a good reinforcement of the preconcentration scheme by sweeping when less-hydrophobic analytes or large negatively-charged molecules need to be preconcentrated.

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Micellar electrokinetic chromatography with polyelectrolyte complexes as micellar pseudo-stationary phases

Cited by 13 publications

References 24 publications

Recent progress in the use of soluble ionic polymers as pseudostationary phases for electrokinetic chromatography

Recent progress in the use of soluble ionic polymers as pseudostationary phases for electrokinetic chromatography

Electrokinetic chromatography with polymeric pseudostationary phases

On-line sample preconcentration by sweeping with dodecyltrimethylammonium bromide in capillary zone electrophoresis

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