Micro‐ and nanostructuring of freestanding, biodegradable, thin sheets of chitosan via soft lithography

Fernandez, Javier G.; Mills, Christopher A.; Martínez, Elena; Lopez-Bosque, Maria J.; Sisquella, Xavier; Errachid, Abdelhamid; Samitier, J.

doi:10.1002/jbm.a.31561

Cited by 23 publications

(17 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The current state-of-the-art bioprinted constructs are still relatively thin layers of cells within a biological hydrogel. 23,34,42,64,71 Although still in its infancy, the technology has provided a method to arrange biological materials into planar and 3D shapes. However, these structures are very simple-containing one or two cell types within a few select matrix proteins.…”

Section: Future Directionsmentioning

confidence: 99%

Direct‐write bioprinting three‐dimensional biohybrid systems for future regenerative therapies

et al. 2011

View full text Add to dashboard Cite

Regenerative medicine seeks to repair or replace dysfunctional tissues with engineered biological or biohybrid systems. Current clinical regenerative models utilize simple uniform tissue constructs formed with cells cultured onto biocompatible scaffolds. Future regenerative therapies will require the fabrication of complex three-dimensional constructs containing multiple cell types and extracellular matrices. We believe bioprinting technologies will provide a key role in the design and construction of future engineered tissues for cell-based and regenerative therapies. This review describes the current state-of-the-art bioprinting technologies, focusing on direct-write bioprinting. We describe a number of process and device considerations for successful bioprinting of composite biohybrid constructs. In addition, we have provided baseline direct-write printing parameters for a hydrogel system (Pluronic F127) often used in cardiovascular applications. Direct-write dispensed lines (gels with viscosities ranging from 30 mPa*s to greater than 600×106 mPa*s) were measured following mechanical and pneumatic printing via three commercially available needle sizes (20ga, 25ga, and 30ga). Example patterns containing microvascular cells and isolated microvessel fragments were also bioprinted into composite 3D structures. Cells and vessel fragments remained viable and maintained in vitro behavior after incorporation into biohybrid structures. Direct-write bioprinting of biologicals provides a unique method to design and fabricate complex, multi-component 3D structures for experimental use. We hope our design insights and baseline parameter descriptions of direct-write bioprinting will provide a useful foundation for colleagues to incorporate this 3D fabrication method into future regenerative therapies.

show abstract

Section: Future Directionsmentioning

confidence: 99%

Direct‐write bioprinting three‐dimensional biohybrid systems for future regenerative therapies

et al. 2011

View full text Add to dashboard Cite

show abstract

“…97 Solidscape (Merrimack, NH) devices print an implant shape in wax that can then be replaced with resorbable materials. 98 Soft lithography (Nanoterra, Cambridge, MA) 99 and electrospinning 100 are technologies that can produce very high-resolution surface features, such as roughness on a scale relevant to cells.…”

Section: Computer-aided Rapid Prototypingmentioning

confidence: 99%

Stereolithographic Bone Scaffold Design Parameters: Osteogenic Differentiation and Signal Expression

Kim

Yeatts

Dean

et al. 2010

Tissue Engineering Part B: Reviews

221

142

View full text Add to dashboard Cite

“…320 While MEMS approaches are very effective at forming complex planar systems, it is difficult to build complex patterns in 3 dimensions. 321 The first efforts at bio-printing involved inkjet printers to dispense cells onto substrates. 322 Inkjet cartridges and nozzles, modified to accommodate cells, dispense droplets of polymer suspensions or cell-polymer suspensions onto a surface.…”

Section: Patterning Vascular Cellsmentioning

confidence: 99%

Manipulating the Microvasculature and Its Microenvironment

Krishnan

Chang

Nunes

et al. 2013

Crit Rev Biomed Eng

View full text Add to dashboard Cite

The microvasculature is a dynamic cellular system necessary for tissue health and function. Therapeutic strategies that target the microvasculature are expanding and evolving, including those promoting angiogenesis and microvascular expansion. When considering how to manipulate angiogenesis, either as part of a tissue construction approach or a therapy to improve tissue blood flow, it is important to know the microenvironmental factors that regulate and direct neovessel sprouting and growth. Much is known concerning both diffusible and matrix-bound angiogenic factors, which stimulate and guide angiogenic activity. How the other aspects of the extravascular microenvironment, including tissue biomechanics and structure, influence new vessel formation is less well known. Recent research, however, is providing new insights into these mechanisms and demonstrating that the extent and character of angiogenesis (and the resulting new microcirculation) is significantly affected. These observations and the resulting implications with respect to tissue construction and microvascular therapy are addressed.

show abstract

Micro‐ and nanostructuring of freestanding, biodegradable, thin sheets of chitosan via soft lithography

Cited by 23 publications

References 38 publications

Direct‐write bioprinting three‐dimensional biohybrid systems for future regenerative therapies

Direct‐write bioprinting three‐dimensional biohybrid systems for future regenerative therapies

Stereolithographic Bone Scaffold Design Parameters: Osteogenic Differentiation and Signal Expression

Manipulating the Microvasculature and Its Microenvironment

Contact Info

Product

Resources

About